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Human Osteopontin,OPN ELISA Kit
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Human Osteopontin,OPN ELISA Kit
Sandwich quantitative immunoassay for secreted phosphoprotein 1 in human serum, plasma, urine, tissue homogenates available in multiple catalog sizes:
Note: Please send inquiries regarding Trial 24T orders to support@diagnocine.com.
| Target name | secreted phosphoprotein 1 |
| Uniprot No. | P10451 |
| Species | Homo sapiens (Human) |
| Sample types | serum, plasma, urine, tissue homogenates |
| Detection range | 0.78 ng/mL-50 ng/mL |
| Sensitivity | 0.195 ng/mL |
| Assay time | 1-5h |
| Sample loading volume | 50-100μL |
| Detection wavelength | 450 nm |
| Assay principle | Sandwich (Quantitative) |
| Data analysis | Standard curve + Curve Expert software |
| Research area | Cancer |
| Storage condition | 2-8°C (see protocol for full details) |
| Shipping condition | 4 °C |
| Shelf life | 6 months |
10 business days
Processing + 3-5 days shipping
In Stock : USA
Worldwide shipping available
Antibody capture
450 nm detection
Standard curve
Quantification
In this sandwich ELISA, secreted phosphoprotein 1 in the sample is captured between a pre-coated capture antibody and a detection antibody. Signal intensity is proportional to analyte concentration. Quantification uses a standard curve fitted with Curve Expert software, covering 0.78 ng/mL-50 ng/mL with a minimum detectable dose of 0.195 ng/mL.
The Human Osteopontin (OPN) ELISA Kit is a powerful tool for researchers in the field of cancer. This quantitative assay measures levels of OPN, a protein involved in the regulation of bone remodeling and implicated in various diseases including cancer（tumor）,bone metabolism, immunity, growth and development.
With a detection range of 0.78 ng/mL-50 ng/mL and a sensitivity of 0.195 ng/mL, this ELISA kit can detect low levels of OPN with high accuracy. It can be used with a variety of sample types, including serum, plasma, urine, and tissue homogenates, making it a versatile tool for research.
- OPN is not only the pivotal protein controlling fibrosis, but may also serve as a biomarker associated with prognosis. PMID: 29120858
- miR-129-5p level was decreased in fibrotic liver of human, and reduced by rOPN treatment. In contrast, miR-129-5p was induced in HSCs transfected by OPN siRNA. These data suggested that OPN induces Col 1 expression via suppression of miR-129-5p in hepatic stellate cells. PMID: 29196165
- plasma level increases in pregnancy independently of asthma PMID: 29200898
- The results suggest that OPN, MMP9 and S100A8 all play a significant role in bladder cancer progression and are potential prognostic markers and therapeutic targets in bladder cancer PMID: 29209142
- these results showed that expression of tear OPN protein by perennial allergic conjunctivitis patients increased significantly compared with controls or seasonal allergic conjunctivitis patients out of the pollen season PMID: 29279263
- Results show that osteopontin is positively associated with hepatocellular carcinoma (HCC) metastasis, and that OPN can activate CCR1 expression through the PI3K/AKT/HIF-1a signaling pathway to promote HCC progression and metastasis. PMID: 29285854
- The study revealed an enhanced sensitivity of aortic valve interstitial cells to osteogenic inductors in aortic stenosis patients, which indicates probable implication of OPN, OPG, and BMP2 genes in pathogenesis of aortic valve calcification. PMID: 29308559
- High circulating osteopontin levels predict major adverse cardiovascular events in patients with severe carotid artery stenosis. PMID: 29317141
- result of this study confirms that increased levels of OPN exist in CSF and peripheral blood of MS patients and strengthens the evidence regarding the clinical utility of OPN as a promising and validated biomarker for MS PMID: 29346446
- First report of OPN cleavage in THP-1 macrophages after PMA (phorbol 12-myristate 13-acetate) stimulation, and of enhanced cleavage induced by BCG infection (bovine tuberculosis). PMID: 29385060
- The expression of OPN was high in endometrium in secretory phase and in vitro decidualized endometrial stromal cells. Further analysis confirmed that OPN expression was upregulated by cAMP and C/EBPbeta signal pathway, while downregulated by miR181b. Increased OPN expression could promote the expression of decidualization-related and angiogenesis-related genes PMID: 29420252
- Serum osteopontin concentrations were increased in acute pancreatitis (AP) compared with follow-up 3 months after discharge. PMID: 29424811
- Plasma OPN levels were associated with the presence and severity of diabetic retinopathy in Asians with type 2 diabetes. PMID: 29463954
- Osteopontin and CD44 play important roles in the development and progression of meningioma and can be used as prognostic markers for tumor recurrence and progression as well as therapeutic targets for the development of new drugs. PMID: 29504367
- Methylglyoxal-bis-guanylhydrazone may have potential therapeutic utility in reducing or normalizing OPN levels and regulating monocyte activation in diseases that involve chronic inflammation. PMID: 29538412
- novel 9175th- (exon 7) position polymorphism of OPN and rs17524488 were related to susceptibility to ankylosing spondylitis in a Chinese population PMID: 29581970
- Respiratory syncytial virus infection leads to increased OPN expression.IL-1beta is involved in the regulation of OPN levels during respiratory syncytial virus infection. PMID: 29677209
- High SPP1 Expression is associated with Hepatocellular Carcinomas. PMID: 29693976
- Serum OPN and DKK1 levels of hepatocellular carcinoma patients could be considered as novel biomarkers showing prognostic significance after hepatectomy based on long-term survival data PMID: 29753515
- Upregulation of ADIPOR1 and SPP1, among the adipokine gene family, in cancer tissue is associated with poor survival in CRC, suggesting a potential mechanism linking obesity and colorectal cancer. PMID: 29761507
- Our data provide evidence that leptin-mediated OPN upregulation promote TH2 inflammation in AR and this process is achieved through the alpha4 integrin and PI3K/AKT signaling pathways. PMID: 29885866
- The serum levels of cystatin C and urine NGAL, urine OPN can be used as a good marker for assessing the renal effect of obesity which can lead end stage renal disease in pediatric population. PMID: 30035656
- Results of the study showed that low urinary OPN levels were correlated with increased kidney stone risk, and dietary habits can affect urinary OPN level PMID: 30114399
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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| To assess the linearity of the assay, samples were spiked with high concentrations of human OPN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:5 | Average % | 94 | ||||
| Range % | 88-98 | |||||
| 1:10 | Average % | 100 | ||||
| Range % | 94-104 | |||||
| 1:20 | Average % | 93 | ||||
| Range % | 88-97 | |||||
| 1:40 | Average % | 99 | ||||
| Range % | 93-104 | |||||
| The recovery of human OPN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 81-97 | ||||
| EDTA plasma (n=4) | 102 | 97-106 | ||||
- A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-human OPN antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
- Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
- One vial Biotin-labeled OPN antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
- One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
- One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
- One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
- A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
- An incubator can provide stable incubation conditions up to 37°C±5°C.
- Centrifuge
- Vortex
- Squirt bottle, manifold dispenser, or automated microplate washer
- Absorbent paper for blotting the microtiter plate
