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Human Interleukin 6,IL-6 ELISA KIT
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* all types of species
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4 core reagents below are included with every order, plus 1 of the 2 detection-specific reagents (matched automatically to your kit type) — 5 components, every time.
+ the 5th component, matched to your kit's detection method:
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All buffers and detection reagents work seamlessly across target proteins, bridging the gap in assay consistency.
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Human Interleukin 6,IL-6 ELISA KIT
Sandwich quantitative immunoassay for interleukin 6 (interferon, beta 2) in human serum, plasma, cell culture supernates, tissue homogenates, urine available in multiple catalog sizes:
Note: Please send inquiries regarding Trial 24T orders to support@diagnocine.com.
| Target name | interleukin 6 (interferon, beta 2) |
| Uniprot No. | P05231 |
| Species | Homo sapiens (Human) |
| Sample types | serum, plasma, cell culture supernates, tissue homogenates, urine |
| Detection range | 7.8 pg/mL-500 pg/mL |
| Sensitivity | 2.453 pg/mL |
| Assay time | 1-5h |
| Sample loading volume | 50-100μL |
| Detection wavelength | 450 nm |
| Assay principle | Sandwich (Quantitative) |
| Data analysis | Standard curve + Curve Expert software |
| Research area | Immunology |
| Storage condition | 2-8°C (see protocol for full details) |
| Shipping condition | 4 °C |
| Shelf life | 6 months |
10 business days
Processing + 3-5 days shipping
In Stock : USA
Worldwide shipping available
Antibody capture
450 nm detection
Standard curve
Quantification
In this sandwich ELISA, interleukin 6 (interferon, beta 2) in the sample is captured between a pre-coated capture antibody and a detection antibody. Signal intensity is proportional to analyte concentration. Quantification uses a standard curve fitted with Curve Expert software, covering 7.8 pg/mL-500 pg/mL with a minimum detectable dose of 2.453 pg/mL.
The human IL-6 ELISA kit is suitable for the quantitative determination of human IL-6 in a wide range of sample types, including serum, plasma, cell culture supernates, tissue homogenates, and urine. This assay employs the bi-antibody sandwich technique and enzyme-substrate chromogenic reaction to quantify antigen levels in the sample. The amount of synthesized colored products are positively related to the analyte of interest in the sample.This human IL-6 ELISA kit has been described in up to 143 publications.
IL-6 is rapidly and transiently generated in response to infections and tissue damage, contributing to host defense via the induction of acute-phase responses, hematopoiesis, and immune reactions. In addition to regulating immune and inflammatory reactions, IL-6 also plays an important role in hematopoiesis, metabolism, and organ development. IL-6 binding to its receptor IL-6R ligates to gp130, initiating a downstream signal cascade, including JAK/STAT3 and JAK-SHP-2-MAPK pathways. Dysregulated IL-6 has been associated with the pathogenesis of autoimmune and inflammatory diseases, metabolic abnormalities, and cancer. Targeting IL-6 has shown to be potential for the treatment of various immune-mediated diseases.
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
To assess the linearity of the assay, samples were spiked with high concentrations of human IL-6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
