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Human Interleukin 16,IL-16 ELISA KIT
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Human Interleukin 16,IL-16 ELISA KIT
Sandwich quantitative immunoassay for interleukin 16 (lymphocyte chemoattractant factor) in human serum, plasma, cell culture supernates, tissue homogenates available in multiple catalog sizes:
Note: Please send inquiries regarding Trial 24T orders to support@diagnocine.com.
| Target name | interleukin 16 (lymphocyte chemoattractant factor) |
| Uniprot No. | Q14005 |
| Species | Homo sapiens (Human) |
| Sample types | serum, plasma, cell culture supernates, tissue homogenates |
| Detection range | 0.156 ng/mL-10 ng/mL |
| Sensitivity | 0.039 ng/mL |
| Assay time | 1-5h |
| Sample loading volume | 50-100μL |
| Detection wavelength | 450 nm |
| Assay principle | Sandwich (Quantitative) |
| Data analysis | Standard curve + Curve Expert software |
| Research area | Immunology |
| Storage condition | 2-8°C (see protocol for full details) |
| Shipping condition | 4 °C |
| Shelf life | 6 months |
10 business days
Processing + 3-5 days shipping
In Stock : USA
Worldwide shipping available
Antibody capture
450 nm detection
Standard curve
Quantification
In this sandwich ELISA, interleukin 16 (lymphocyte chemoattractant factor) in the sample is captured between a pre-coated capture antibody and a detection antibody. Signal intensity is proportional to analyte concentration. Quantification uses a standard curve fitted with Curve Expert software, covering 0.156 ng/mL-10 ng/mL with a minimum detectable dose of 0.039 ng/mL.
The ELISA Kit is designed for quantitatively measuring human IL-16 levels in samples, including serum, plasma, cell culture supernates, or tissue homogenates. It uses the sandwich enzyme immunoassay technique in combination with the enzyme-substrate chromogenic reaction to quantify the analyte in the sample. The color develops positively to the amount of IL-16 in samples. The color intensity is measured at 450 nm via a microplate reader.
IL-16 is known to induce the IL-2Rα on T cells, suppress HIV replication and inhibit T-cell antigen receptor/CD3 mediated T-cell stimulation in mixed lymphocyte reactions. In addition, IL-16 acts in concert with IL-2 and/or IL-15 to promote CD4 + T-cell proliferation, and is a chemoattractant factor for CD4 + cells. IL-16 plays a key role in airway hyper-responsiveness and up-regulation of IgE. IL-16 has been associated with diseases characterized by CD4 + T-cell involvement, such as allergic asthma, rheumatoid arthritis, multiple sclerosis, and acquired immunodeficiency syndrome.
- IL16 polymorphisms may play a role in the pathophysiology of alopecia areata or in the expression of phenotypes in Koreans. PMID: 24320753
- The rs11556218 T/G polymorphism of the IL-16 gene was significantly associated with elevated cancer risk in Asian populations. [meta-analysis] PMID: 24328527
- This study showed that tissue expression and serum levels of IL-16 increase in association with malignant ovarian tumor development and progression. PMID: 24380743
- IL-16 rs4778889 T/C polymorphism is not associated with cancer. PMID: 24568499
- The IL-16 SNP rs8028364 was found to be associated with Graves' disease(GD) when compared with the control subjects. PMID: 24694201
- The production of IL-16 contributed to lung damage as neutralization of IL-16 enhanced S. aureus clearance and resulted in diminished lung pathology in S. aureus pneumonia. PMID: 24736233
- Data suggest that thymic stromal lymphopoietin (TSLP) and interleukin-16 (IL-16), expressed at early stages of disease, function to recruit malignant T cells to the skin. PMID: 24794807
- IL-16 rs11556218 T/G was associated with a significant increased risk of cancer. PMID: 24969906
- The findings suggest that the IL-16 rs 11556218 polymorphism may be used as a susceptibility marker for glioma. PMID: 25166752
- intrathyroidal production of IL-14 and IL-16 associated with the pathogenesis of autoimmune thyroid disease PMID: 25940130
- The results suggested that the variants in IL-16 gene rs11556218 site were associated with a decreased knee osteoarthritis risk after adjusting for age, sex, BMI, and smoking and drinking status. PMID: 25954818
- the lack of a relationship between genotype and serum levels of IL-16 seems to suggest a more complex regulation of this cytokine at the post-translational level and/or the interplay with other cytokines. PMID: 26154181
- We found that the rs1131445 T/C and rs4072111 T/C variants of IL-16 were significantly associated with increased risk of gastric cancer in Iranian population. PMID: 26346169
- The IL16 rs4072111 polymorphism may be associated with susceptibility to AD and the T allele may have a protective role in the progression of AD in an Iranian population. PMID: 26386715
- We observed highly significant reductions in the concentration of circulating interleukin (IL)-16, IL-7, and Vascular Endothelial Growth Factor A (VEGF-A) in encephalomyelitis/chronic fatigue syndrome patients. PMID: 26615570
- the results of our study suggested an association between the IL-16 rs4778889 polymorphism and an elevated risk of renal cell carcinoma PMID: 26823871
- IL-16 rs1131445 C/T polymorphism is related to the susceptibility to cancer in Asians (meta-analysis) PMID: 26842762
- Structure of a Potential Therapeutic Antibody Bound to Interleukin-16 PMID: 27231345
- No significant differences were observed in rs11556218 genotype frequencies between the study groups. In conclusion, the results of our study reveal an association between the IL-16 rs4778889 polymorphism and heightened risk of renal cell cancer . PMID: 27323152
- Data indicate an association between interleukin-16 (IL-16) rs4072111 and rs1131445 with progression to the severe stages (III-IV) of endometriosis. PMID: 27484651
- Long-term exposure to tobacco smoke does not markedly alter extracellular concentrations of IL-16 protein in blood. However, it does decrease the intracellular IL-16 concentrations in blood NK cells. PMID: 27695312
- Association of interleukin 16 gene polymorphisms and plasma IL16 level with osteosarcoma risk. PMID: 27703190
- data revealed an association between circulating IL-16 and severity of psoriasis which indicates that this cytokine could serve as a potential marker of disease activity. However, further investigations are required PMID: 27788245
- rs11556218 T/G genotype associated with increased risk for development of osteoporosis in Chinese postmenopausal women PMID: 27984748
- There was a decreased frequency of IL16 rs4778889 CC genotype carriers among women with gestational diabetes mellitus, however, there was no significant difference in the distribution of alleles. PMID: 28580570
- Results suggest that IL16 genotype predict higher risk of death and progression, respectively, in NSCLC patients PMID: 28807247
- serum IL-16 level may not only serve as a useful diagnostic biomarker for patients with GC, but also may predict cancer recurrence and patient prognosis. PMID: 28990054
- rs11556218 is associated with endometriosis in Greek women, probably by resulting in the aberrant expression of IL16 PMID: 29328375
- Serum IL-6, serum YKL-40, and plasma VEGF were significantly correlated with disease activity at baseline in early rheumatoid arthritis. PMID: 29336711
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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| To assess the linearity of the assay, samples were spiked with high concentrations of human IL-16 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 88 | |||||
| Range % | 83-97 | ||||||
| 1:2 | Average % | 97 | |||||
| Range % | 91-104 | ||||||
| 1:4 | Average % | 84 | |||||
| Range % | 81-88 | ||||||
| 1:8 | Average % | 89 | |||||
| Range % | 81-96 | ||||||
| The recovery of human IL-16 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 101 | 94-101 | |||||
| EDTA plasma (n=4) | 94 | 90-98 | |||||
