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Human Epithelial neutrophil activating peptide 78 (ENA-78/CXCL5) ELISA Kit
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Human Epithelial neutrophil activating peptide 78 (ENA-78/CXCL5) ELISA Kit
Sandwich quantitative immunoassay for Human Epithelial neutrophil activating peptide 78 (ENA-78/CXCL5) in human serum, plasma, tissue homogenates available in multiple catalog sizes:
Note: Please send inquiries regarding Trial 24T orders to support@diagnocine.com.
| Uniprot No. | P42830 |
| Species | Homo sapiens (Human) |
| Sample types | serum, plasma, tissue homogenates |
| Detection range | 47 pg/mL-3000 pg/mL |
| Sensitivity | 23.5 pg/mL |
| Assay time | 1-5h |
| Sample loading volume | 50-100μL |
| Detection wavelength | 450 nm |
| Assay principle | Sandwich (Quantitative) |
| Data analysis | Standard curve + Curve Expert software |
| Research area | Immunology |
| Storage condition | 2-8°C (see protocol for full details) |
| Shipping condition | 4 °C |
| Shelf life | 6 months |
10 business days
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In Stock : USA
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Antibody capture
450 nm detection
Standard curve
Quantification
In this sandwich ELISA, Human Epithelial neutrophil activating peptide 78 (ENA-78/CXCL5) in the sample is captured between a pre-coated capture antibody and a detection antibody. Signal intensity is proportional to analyte concentration. Quantification uses a standard curve fitted with Curve Expert software, covering 47 pg/mL-3000 pg/mL with a minimum detectable dose of 23.5 pg/mL.
This ELISA kit provides quantitative measurement of CXCL5 (ENA-78) in human samples, supporting research into neutrophil recruitment and CXC chemokine signaling in inflammatory and tumor microenvironments. Because CXCL5 levels can vary dramatically between healthy baseline and active inflammatory or tumorigenic states, the kit is designed to capture this biologically meaningful dynamic range without excessive sample manipulation.
Detection Range: The 47–3000 pg/mL range spans from low baseline circulating CXCL5 concentrations in healthy plasma to the elevated levels observed in sepsis, COPD exacerbations, and tumor-associated angiogenesis, minimizing the need for serial dilutions in most inflammatory disease samples.
- our data showed that the CXCR2/CXCL5 axis contributes to EMT of HCC cells through activating PI3K/Akt/GSK-3beta/Snail signaling, and it may serve as a potential therapeutic target. PMID: 25462858
- the increased level of CXCL5 in tissue compartments, including the central nervous system of HIV-1-infected individuals might alter the inflammatory response through the infiltration of neutrophils into tissue compartment PMID: 25536401
- This study aims to evaluate serum levels of ENA78/CXCL5 and SDF-1/CXCL12 along the gastric cancer carcinogenesis, and analyze their clinical significance, and diagnostic potentials through human serum samples. PMID: 25689618
- Analysis of monocultured dermal fibroblasts and keratinocytes revealed that only fibroblasts but not keratinocytes displayed up regulated CXCL5 levels after UV stimulation. PMID: 25690483
- High CXCL5 expression is associated with pediatric ulcerative colitis. PMID: 25738378
- There was an inverse correlation between DACH1 mRNA levels and CXCL5 in both lung cancer cell lines and human NSCLC tissues. PMID: 25788272
- Serum levels of ENA-78 were elevated in autistic children and they were significantly associated with the increased levels of serum antineuronal auto-antibodies PMID: 25871636
- Study shows that CXCL5 expression is elevated in positive correlation to bladder cancer grade and promotes cell migration and invasion via binding to its receptor CXCR2. PMID: 26058729
- CXCL5 expression is enriched in human atherosclerotic coronary artery. The CXCL5 variant might be a genetic risk factor for the susceptibility of CAD and the CXCL5 promoter -156 G/C C allele might be an independent predictor for CAD. PMID: 26287498
- CXCL5 expression in urine is related to bladder cancer TNM stage, lymph node metastasis, tumor size, and tumor grade. PMID: 26503215
- ENA 78 plasma levels were correlated with Expanded Disability Status Scale scores in neuromyelitis optica (NMO) patients; elevated secretion of ENA 78 may be a critical step in neutrophil recruitment during the remission of NMO. PMID: 27401736
- a finely tuned balance between the GAG-bound dimer and free soluble monomer regulates CXCL5-mediated receptor signaling and function. PMID: 27471273
- Serum CXCL5 levels from pemphigus vulgaris patients are significantly higher than those in bullous pemphigoid patients and healthy controls. PMID: 27501402
- Curcumin suppressed CXCL5 expression by direct inhibition of IKKbeta phosphorylation, and inhibition of p38 MAPK via induction of negative regulator MKP-1. PMID: 27538525
- CXCL5 plays a promoting role in glioma in autocrine- and paracrine-dependent manners. PMID: 27748886
- Mechanistically, AR modulated cytokine CXCL5 expression by altering AKT --> NF-kappaB signaling, and interruption of AKT --> NF-kappaB --> CXCL5 signaling using either specific inhibitors or siRNA suppressed AR-enhanced EC recruitment and AR-EC-promoted RCC progression. PMID: 27848972
- CXCL5 levels were decreased in LSCC patient serum. PMID: 27876461
- The expression of CXCL5 is up-regulated in osteosarcoma cells. PMID: 28277189
- our findings support CXCL5 as a promoter of colorectal cancer metastasis and a predictor of poor clinical outcomes in colorectal cancer patients. PMID: 28356111
- findings for the first time provided evidence that ENA78 may play a key role of mediator in pathogenesis of Major Depressive Disorder(MDD) and in the mechanism of vinlafaxine effects on MDD. PMID: 28441588
- The CXCL5 and the overexpression of miR-141 reduced levels of MMP-2 and MMP-9 in tumor necrosis factor-alpha-treated HT29 cells by means of repressing the inhibitory AKT. PMID: 28854064
- PERK-p-eIF2alpha pathway could suppress metastasis in triple-negative breast cancer by inhibiting expression of PDL1 and CXCL5 in tumor cells. PMID: 29053414
- activated CXCL5-CXCR2 axis contributes to the metastatic phenotype of PTC cells by modulating Akt/GSK-3beta/beta-catenin pathway PMID: 29471001
- study elucidates the important role of CXCL5 in the progression and prognosis of NSCLC. These findings suggested that CXCL5 might be a potential biomarker and novel therapeutic target for lung cancer PMID: 29526026
- CXCL5 may promote mitomycin resistance by activating EMT and NF-kappaB pathway. Thus, this study identifies CXCL5 as a novel chemoresistance-related marker in non-muscle invasive bladder cancer PMID: 29545183
- These data demonstrated that CXCL5 expression was upregulated in prostate cancer tissues and that exogenous CXCL5 protein exposure or CXCL5 overexpression promoted malignant phenotypes of prostate cancer cells in vitro and in vivo. PMID: 29749439
Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
Inter-assay Precision (Precision between assays): CV%<10% | ||||||
Three samples of known concentration were tested in twenty assays to assess. | ||||||
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| |||||||
pg/ml | OD1 | OD2 | Average | Corrected | |||
3000 | 3.093 | 2.934 | 3.013 | 2.831 | |||
1500 | 2.440 | 2.567 | 2.504 | 2.322 | |||
750 | 1.797 | 1.828 | 1.813 | 1.630 | |||
375 | 1.248 | 1.312 | 1.280 | 1.098 | |||
187.5 | 0.604 | 0.621 | 0.613 | 0.430 | |||
94 | 0.386 | 0.373 | 0.380 | 0.198 | |||
47 | 0.292 | 0.284 | 0.288 | 0.106 | |||
0 | 0.179 | 0.185 | 0.182 |
| |||
To assess the linearity of the assay, samples were spiked with high concentrations of human ENA-78/CXCL5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | ||||
1:1 | Average % | 99 | ||||
Range % | 92-106 | |||||
1:2 | Average % | 95 | ||||
Range % | 88-102 | |||||
1:4 | Average % | 89 | ||||
Range % | 84-95 | |||||
1:8 | Average % | 92 | ||||
Range % | 85-99 | |||||
The recovery of human ENA-78/CXCL5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 90 | 84-96 | ||||
EDTA plasma (n=4) | 94 | 90-99 | ||||