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Human Endostatin,ES ELISA Kit
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Human Endostatin,ES ELISA Kit
Quantitative immunoassay for Endostatin,ES in human serum, plasma, tissue homogenates available in multiple catalog sizes:
Note: Please send inquiries regarding Trial 24T orders to support@diagnocine.com.
| Target name | Endostatin,ES |
| Species | Homo sapiens (Human) |
| Sample types | serum, plasma, tissue homogenates |
| Detection range | 15.6 pg/ml -1000 pg/ml |
| Sensitivity | 3.9 pg/ml |
| Assay time | 1-5h |
| Sample loading volume | 50-100μL |
| Detection wavelength | 450 nm |
| Assay principle | Quantitative |
| Data analysis | Standard curve + Curve Expert software |
| Research area | Cardiovascular |
| Storage condition | 2-8°C (see protocol for full details) |
| Shipping condition | 4 °C |
| Shelf life | 6 months |
10 business days
Processing + 3-5 days shipping
In Stock : USA
Worldwide shipping available
Immunoreaction
450 nm detection
Standard curve
Quantification
Quantification uses a standard curve fitted with Curve Expert software, covering 15.6 pg/ml -1000 pg/ml with a minimum detectable dose of 3.9 pg/ml.
The product CSB-E07973h is a sandwich ELISA kit developed to measure levels of human endostatin (ES) in serum, plasma, or tissue homogenates. The enzyme-substrate chromogenic reaction is also used to amplify the signal and quantify the levels of the analyte through the intensity of the colored product. The color intensity positively correlates with the amount of endostatin bound in the initial step.
Endostatin, a 20-kDa C-terminal cleavage product of type XVIII collagen, is an endogenous inhibitor known for its ability to inhibit the proliferation and migration of endothelial cells, angiogenesis, and tumor growth. Studies have proved that circulating endostatin increases significantly in patients with kidney and heart failure and may also contribute to disease progression. Endostatin inhibits angiogenesis by directly binding to both VEGFR1 and VEGFR2 and blocking VEGF interaction with Flt-1 and Flk-1 to prevent VEGF-induced tyrosine phosphorylation of VEGFR1 and VEGFR2 and all downstream signaling events.
Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
Inter-assay Precision (Precision between assays): CV%<10% | ||||||
Three samples of known concentration were tested in twenty assays to assess. | ||||||
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| |||||||
pg/ml | OD1 | OD2 | Average | Corrected | |||
1000 | 1.976 | 2.034 | 2.005 | 1.827 | |||
500 | 1.120 | 1.167 | 1.143 | 0.966 | |||
250 | 0.751 | 0.728 | 0.740 | 0.562 | |||
125 | 0.494 | 0.519 | 0.506 | 0.329 | |||
62.5 | 0.337 | 0.321 | 0.329 | 0.151 | |||
31.2 | 0.242 | 0.253 | 0.247 | 0.070 | |||
15.6 | 0.221 | 0.214 | 0.218 | 0.040 | |||
0 | 0.180 | 0.175 | 0.178 |
| |||
To assess the linearity of the assay, samples were spiked with high concentrations of human ES in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | ||||
1:200 | Average % | 87 | ||||
Range % | 80-94 | |||||
1:400 | Average % | 93 | ||||
Range % | 88-100 | |||||
1:800 | Average % | 92 | ||||
Range % | 86-100 | |||||
1:1600 | Average % | 90 | ||||
Range % | 86-98 | |||||
The recovery of human ES spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 93 | 89-98 | ||||
EDTA plasma (n=4) | 94 | 88-100 | ||||