Broad Range dsDNA Quantification Kit, 100 assays - for Accuris™ Smart-Q™ Fluorometer
Broad Range dsDNA Quantification Kit, 100 assays - for Accuris™ Smart-Q™ Fluorometer
Cat. No.:BM-F1000-BR-100
Description
The Accuris BR dsDNA Quantitation Kit provides easy and accurate quantitation for dsDNA. The kit includes concentrated assay reagent, dilution buffer, and prediluted DNA standards. The assay kit is highly selective for dsDNA due to fluorescence dye high quantum yield and large molar extinction coefficient.
The kit is highly reliable in detecting dsDNA with initial sample concentrations from 0.2ng/μL to 2000 ng/μL ranging from 4.0 to 2000 ng. The kit offers advantages in stability, linear dynamic range, and sensitivity over other traditional of DNA quantitation. The assay is performed at room temperature.
The reagent is diluted using the buffer provided, added your sample (any volume between 1 μL and 20 μL is acceptable), and the fluorescence is read using a fluorometer.
Kit Components & Storage Requirements
| Material | Storage | Amount | Concentration |
| Accuris BR dsDNA Reagent (Component 1) |
4 ℃ Protect from light |
100 μL | 200 X in DMSO |
| Accuris BRdsDNA Buffer (Component 2) |
4 ℃ | 25 mL | 10 X |
| Accuris BR dsDNA Standard #1 (Component 3) |
4 ℃ | 200 μL | 0 ng/μL |
| Accuris BR dsDNA Standard #2 (Component 4) |
4 ℃ | 200 μL | 100 ng/μL |
Package contents and reordering
Accuris Broad Range dsDNA Quantification Kit, 100 assays - Catalog number NS1020-BR-100
General Protocol
Preparation
1. Warm the Accuris Broad Range dsDNA Quantitation Kit to room temperature. Check the BR dsDNA reagent for any precipitation. If precipitation is seen, warm up the vial in a water bath and vortex until dissolved.
2. Prepare 1× BR dsDNA Buffer by diluting the 10× BR dsDNA Buffer 1:9 in deionized water.
3. Prepare the working solution by diluting the BR dsDNA reagent 1:199 in 1× BR dsDNA buffer. Use a clean plastic tube each time to make working solution. For example, to measure 8 samples in duplicate, add 10 μL of BR dsDNA reagent to 1990 μL of BR dsDNA Buffer. Mix well and use IMMEDIATELY. Once mixed into the working solution, samples must be measured within 3 hours to prevent degradation of fluorescence intensity.
Standard Curve
1. Add 190 μL of the working solution to each assay tube. (Note: Use only clear 0.5 mL PCR tubes for fluorescence analysis.
2. Add 10 μL of dsDNA standard #1 (Component 3), dsDNA standard #2 (Component 4) into separated tubes, and mix by vortexing (5-10 seconds), and incubate all tubes at room temperature for 3 minutes in the dark. Note: When mixing dsDNA standard and working solution, please disperse the bubbles before analysis.
3. Measure the fluorescence using the calibration program of standard curve. Click dsDNA in the Home interface, select “dsDNA: Broad Range” and press the button. Select “Calibration” from the pop-up box. Standard 1 should be set by default as having 0 concentration. Insert standard 1 into the fluorometer port and click “Read standards” to perform the measurement. Once finished, proceed to set up and measure standard 2. After calibration, samples are ready to be measured.
Measuring Samples
1. Add the sample (any volume between 1 μL and 20 μL is acceptable) and the working solution, and the final volume ineach tube should be 200 μL.
2. Mix by vortexing (5-10 seconds). Incubate all tubes at room temperature for 3 minutes in the dark. Note: When mixing dsDNA standard and working solution, please disperse the bubbles before analysis.
3. Place the sample into the fluorometer for measurement.
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Smart-Q Fluorometer, for quantification of DNA, RNA and protein samples
- Sensitive quantitation down to 0.1pg/ul ds DNA
- Large color touch screen for programming and operation
- Two models for single or 8-sample processing
- Built-in reagent calculators for sample preparation
- Compact and portable (11 x 6 inch footprint)
Smart-Q Specifications
| Model | Smart-Q™ 100 Fluorometer | Smart-Q™ 800 Fluorometer | Smart-Q™ Mini 115V | Smart-Q™ Mini 230V |
|---|---|---|---|---|
| Display | 7.0-inch, color touch screen | 7.0-inch, color touch screen | No external display (instrument operation via iOS/Android phone or tablet) |
No external display (instrument operation via iOS/Android phone or tablet) |
| Dynamic Range | 5 orders of magnitude | 4 orders of magnitude | 5 orders of magnitude | 5 orders of magnitude |
| Light Source | Blue and Red LED | Blue and Red LED | Blue and Red LED | Blue and Red LED |
| Excitation Wavelengths | Blue: 460 ± 20nm and Red: 625 ± 20nm | Blue: 460 ± 20nm and Red: 625 ± 20nm | Blue: 470 ± 15 nm Red: 620 ± 20 nm | Blue: 470 ± 15 nm Red: 620 ± 20 nm |
| Emission Wavelengths | Green: 525 – 570nm and Red: 670 – 725nm | Green: 525 – 570nm and Red: 670 – 725nm | Green: 525 – 570nm and Red: 670 – 725nm | Green: 525 – 570nm and Red: 670 – 725nm |
| Stability | ≤ 1.5% | ≤ 1.5% | ≤1.5% | ≤ 1.5% |
| Detector | Photodiodes (300-1000nm) | Photodiodes (300-1000nm) | Photodiodes (320-1100nm) | Photodiodes (320-1100nm) |
| Measurement Speed | ≤ 5 seconds | ≤ 6 seconds | ≤ 5 seconds | ≤ 5 seconds |
| Consumable type | 0.5mL assay tubes | 0.2mL assay tubes (single and 8-tube strip) | 0.5 mL optically-clear quantification tubes | 0.5 mL optically-clear quantification tubes |
| Input Voltage | 24 VDC 2A | 24 VDC 2A | 115V | 230V |
| Dimensions (WxDxH) | 16.1 x 28.7 x 6.1cm / 6.34 x 11.30 x 2.40in. | 16.1 x 28.7 x 7.6 cm / 6.3 x 11.3 x 3.0 in. | 3.27 x 3.27 x 3.62 in. / 8.13 x 8.13 x 3.19 cm | 3.27 x 3.27 x 3.62 in. / 8.13 x 8.13 x 3.19 cm |
| Weight | 1.6kg / 3.5lbs | 2.0kg / 4.4lbs | 0.5kg / 1.1lbs | 0.5kg / 1.1lbs |
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Description
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The Accuris™ Smart-Q™ 100 & Smart-Q™ 800 Fluorometers are designed for the precise quantification of DNA, RNA, and protein samples. By utilizing well-established methodologies for fluorescence detection, the Smart-Q Fluorometers provide higher specificity and enhanced accuracy compared to the traditional method of UV-absorbance detection. Accurate results are particularly crucial in workflows involving:
ssDNA Quantification KitThe Accuris ssDNA Kit provides a simple and accurate method for quantifying single-stranded DNA (ssDNA) or oligonucleotides. The assay kit is accurate at detecting ssDNA between 0.05-200 ng/µL. While the kit is not selective for ssDNA over double-stranded DNA (dsDNA) or RNA, it does not detect contaminating proteins or nucleotides. Additionally, the kit demonstrates insensitivity to low concentrations of common contaminants such as proteins, salts, solvents, and detergents RNA High Sensitivity Quantification KitThe Accuris RNA High Sensitivity Quantification Kit offers a reliable and accurate method for measuring RNA concentration. This assay is highly selective for RNA over double-stranded and single-stranded DNA and can detect RNA concentrations ranging from 0.1 ng/μL to 100 ng/μL RNA Broad Range Quantification KitThe Accuris RNA Broad Range Quantification Kit enables precise and reliable RNA quantification. This assay exhibits high specificity for RNA over single stranded and double-stranded DNA, allowing for the detection of RNA concentrations ranging from 1 ng/μL to 1000 ng/μL. This assay demonstrates robustness against common contaminants, including proteins, free nucleotides, salts, solvents, and detergents |
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| Smart-Q 100 for single sample processing | |
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| Smart-Q 800 for up to 8-sample processing | |
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| Accuris DNA Quantification Kits | |
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Ordering Information
| Smart-Q™ | Cat.No. | Products | Qty ( Pack ) | Order |
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BM-F1001 | Smart-Q 100 Fluorometer, single sample | 1 | |
| BM-F1008 | Smart-Q 800 Fluorometer, 8-channel1 | 1 | |
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BM-F0100 | Smart-Q Mini Fluorometer, 115V | 1 | |
| BM-F0100-E | Smart-Q Mini Fluorometer, 230V | 1 | |
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BM-F0100-TAB | Android tablet preloaded with Smart-Q Mini app | 1 | |
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BM-F1000-HS-100 | High Sensitivity dsDNA Quantification Kit, 100 assays | 1 Kit | |
| BM-F1000-HS-500 | High Sensitivity dsDNA Quantification Kit, 500 assays | 1 Kit | |
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| BM-F1000-HS1-100 | 1X High Sensitivity dsDNA Quantification Kit, 100 assays | 1 Kit | |
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| BM-F1000-HS1-500 | 1X High Sensitivity dsDNA Quantification Kit, 500 assays | 1 Kit | |
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| BM-F1000-BR-100 | Broad Range dsDNA Quantification Kit, 100 assays | 1 Kit | Current page | |
| BM-F1000-BR-500 | Broad Range dsDNA Quantification Kit, 500 assays | 1 Kit | |
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| BM-F1000-BR1-100 | 1X Broad Range dsDNA Quantification Kit, 100 assays | 1 Kit | |
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| BM-F1000-BR1-500 | 1X Broad Range dsDNA Quantification Kit, 500 assays | 1 Kit | |
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| BM-F1000-SD-100 | ssDNA Quantification Kit, 100 Assays | 1 Kit | |
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| BM-F1000-SD-500 | ssDNA Quantification Kit, 500 Assays | 1 Kit | |
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| BM-F1000-RHS-100 | High Sensitivity RNA Quantification Kit, 100 assays | 1 Kit | |
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| BM-F1000-RHS-500 | High Sensitivity RNA Quantification Kit, 500 assays | 1 Kit | |
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| BM-F1000-RBR-100 | Broad Range RNA Quantification Kit, 100 assays | 1 Kit | |
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| BM-F1000-RBR-500 | Broad Range RNA Quantification Kit, 500 assays | 1 Kit | |
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BM-F1001-T | 0.5mL Fluorescence Detection Tubes, pack of 250 | 250 | |
| BM-F1008-T | 0.2ml Smart-Q™ 800 Assay Tubes, pack of 1000 | 1000 | |
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| BM-F1008-T-8 | 0.2ml Smart-Q™ 800 Assay Tube Strips, pack of 120 | 120 | |
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