1X Broad Range dsDNA Quantification Kit, 100 assays
1X Broad Range dsDNA Quantification Kit, 100 assays
Cat. No.: BM-F1000-BR1-100
Description
The Accuris 1X BR Double-Stranded DNA (dsDNA) Quantification Kit provides a highly sensitive, precise, and efficient method for quantifying dsDNA concentrations. This kit includes a ready-to use assay working solution and dsDNA standards, ensuring both reproducibility and ease of application.
Designed for optimal specificity, the assay selectively binds to dsDNA, detecting concentrations ranging from 2 to 1000 ng, thereby offering exceptional stability, an extended linear dynamic range, and enhanced sensitivity compared to conventional DNA quantification techniques. The assay is conducted at room temperature by simply mixing the sample with the working solution and measuring fluorescence using either a fluorometer or a fluorescent plate reader (for plate reader-specific instructions, please contact the manufacturer).
Additionally, the kit demonstrates high resistance to common contaminants, including proteins, salts, solvents, and detergents, ensuring robust and reliable performance across diverse experimental conditions.
Kit Components & Storage Requirements
| Material | Storage | Amount | Concentration | Stabiliy |
| Accuris 1X BR dsDNA Working Solution (Component A) |
4 ℃ Protect from light |
20 mL | 1x | Product Stable for at least 6 months when stored as directed |
| Accuris 1X BR dsDNA Standard 1 (Component B) |
0.2 mL | 0 ng/μL in TE Buffer |
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| Accuris 1X BR dsDNA Standard 2 (Component C) |
0.2 mL | 100 ng/μL in TE Buffer |
Critical Assay Parameters:
Incubation Time: For optimal detection of target molecules, incubate the standards and samples with the Accuris 1X BR dsDNA working solution for 2–3 minutes in the dark at room temperature.
Fluorometer Calibration: Users may utilize a previous calibration or perform a new calibration before each assay. However, we recommend conducting a new calibration for optimal accuracy.
Assay Temperature: The Accuris 1X BR dsDNA Quantification Kit performs optimally at room temperature. Therefore, all reagents should be fully equilibrated to room temperature before use to ensure accurate results.
Assay Selectivity: The Accuris 1X BR dsDNA Quantification Kit exhibits high tolerance to common contaminants, including proteins, salts, solvents, and detergents, ensuring reliable performance across various sample conditions.
Figure: The data represents DNA quantification using the Accuris 1X BR dsDNA Quantification kit.
Standards and Sample Preparation:
This protocol provides instructions on how to use the Accuris 1X BR dsDNA Quantification kit with the Accuris Smart Q and Qubit Fluorometers
1. Allow all components of the Accuris 1X BR dsDNA quantification kit to reach room temperature.
2. Prepare the required number of assay tubes accounting for the two standards (Use only thin-wall , clear 0.5 mL PCR tubes).
3. Label the tubes on the lid.
4. Add 190 μL of the Accuris 1x BR dsDNA Working solution to each standard tube and 180-199 μL to sample tubes dpendent on on the volume of unknown sample (refer to table below).
5. Add 10 μL of dsDNA Standard 1 and Standard 2 and user defined volume ofsamples to corresponding tubes (refer to image below).
6. Mix samples by vortexing tubes for 3 seconds and incubate all tubes at toom temperature for 1-3 minutes, thereafter.
Standards and Sample Measurements
Standard Curve:
1. On the Home screen, select dsDNA, then choose dsDNA: Broad.
2. Insert standard 1 into a reading chamber and select start symbol followed by pressing the read symbol.
3. After selecting concentration units, choose Read Standards.
4. Replace standard tube 1 with standard 2 and select Read standards to obtain reading for the second standard.
5. Select Save to save the standard curve. Note: this curve may be re-used for subsequent sample readings. We recommend to prepare new standard curve each time assay is performed
Sample Quantification:
1. On the fluorometer screen, enter the volume of the unknown sample, then select the Start icon.
2. Once the fluorometer completes the quantification, the sample concentration and Relative Fluorescence Units (RFU) will be displayed on the screen.
3. Remove the sample, insert the next sample into the test chamber, and select the Start icon.
4. Repeat Step 3 until all samples have been quantified. Note: At any point during quantification, the fluorometer screen can be swiped left to display all recorded concentrations from the current experiment, with options to print or export the results. Selecting the Calculator function allows users to calculate the molarity of the sample (note: the DNA length must be entered for accurate quantification).
5. After completing the quantification process, turn off the fluorometer.
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Smart-Q Fluorometer, for quantification of DNA, RNA and protein samples
- Sensitive quantitation down to 0.1pg/ul ds DNA
- Large color touch screen for programming and operation
- Two models for single or 8-sample processing
- Built-in reagent calculators for sample preparation
- Compact and portable (11 x 6 inch footprint)
Smart-Q Specifications
| Model | Smart-Q™ 100 Fluorometer | Smart-Q™ 800 Fluorometer | Smart-Q™ Mini 115V | Smart-Q™ Mini 230V |
|---|---|---|---|---|
| Display | 7.0-inch, color touch screen | 7.0-inch, color touch screen | No external display (instrument operation via iOS/Android phone or tablet) |
No external display (instrument operation via iOS/Android phone or tablet) |
| Dynamic Range | 5 orders of magnitude | 4 orders of magnitude | 5 orders of magnitude | 5 orders of magnitude |
| Light Source | Blue and Red LED | Blue and Red LED | Blue and Red LED | Blue and Red LED |
| Excitation Wavelengths | Blue: 460 ± 20nm and Red: 625 ± 20nm | Blue: 460 ± 20nm and Red: 625 ± 20nm | Blue: 470 ± 15 nm Red: 620 ± 20 nm | Blue: 470 ± 15 nm Red: 620 ± 20 nm |
| Emission Wavelengths | Green: 525 – 570nm and Red: 670 – 725nm | Green: 525 – 570nm and Red: 670 – 725nm | Green: 525 – 570nm and Red: 670 – 725nm | Green: 525 – 570nm and Red: 670 – 725nm |
| Stability | ≤ 1.5% | ≤ 1.5% | ≤1.5% | ≤ 1.5% |
| Detector | Photodiodes (300-1000nm) | Photodiodes (300-1000nm) | Photodiodes (320-1100nm) | Photodiodes (320-1100nm) |
| Measurement Speed | ≤ 5 seconds | ≤ 6 seconds | ≤ 5 seconds | ≤ 5 seconds |
| Consumable type | 0.5mL assay tubes | 0.2mL assay tubes (single and 8-tube strip) | 0.5 mL optically-clear quantification tubes | 0.5 mL optically-clear quantification tubes |
| Input Voltage | 24 VDC 2A | 24 VDC 2A | 115V | 230V |
| Dimensions (WxDxH) | 16.1 x 28.7 x 6.1cm / 6.34 x 11.30 x 2.40in. | 16.1 x 28.7 x 7.6 cm / 6.3 x 11.3 x 3.0 in. | 3.27 x 3.27 x 3.62 in. / 8.13 x 8.13 x 3.19 cm | 3.27 x 3.27 x 3.62 in. / 8.13 x 8.13 x 3.19 cm |
| Weight | 1.6kg / 3.5lbs | 2.0kg / 4.4lbs | 0.5kg / 1.1lbs | 0.5kg / 1.1lbs |
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Description
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The Accuris™ Smart-Q™ 100 & Smart-Q™ 800 Fluorometers are designed for the precise quantification of DNA, RNA, and protein samples. By utilizing well-established methodologies for fluorescence detection, the Smart-Q Fluorometers provide higher specificity and enhanced accuracy compared to the traditional method of UV-absorbance detection. Accurate results are particularly crucial in workflows involving:
ssDNA Quantification KitThe Accuris ssDNA Kit provides a simple and accurate method for quantifying single-stranded DNA (ssDNA) or oligonucleotides. The assay kit is accurate at detecting ssDNA between 0.05-200 ng/µL. While the kit is not selective for ssDNA over double-stranded DNA (dsDNA) or RNA, it does not detect contaminating proteins or nucleotides. Additionally, the kit demonstrates insensitivity to low concentrations of common contaminants such as proteins, salts, solvents, and detergents RNA High Sensitivity Quantification KitThe Accuris RNA High Sensitivity Quantification Kit offers a reliable and accurate method for measuring RNA concentration. This assay is highly selective for RNA over double-stranded and single-stranded DNA and can detect RNA concentrations ranging from 0.1 ng/μL to 100 ng/μL RNA Broad Range Quantification KitThe Accuris RNA Broad Range Quantification Kit enables precise and reliable RNA quantification. This assay exhibits high specificity for RNA over single stranded and double-stranded DNA, allowing for the detection of RNA concentrations ranging from 1 ng/μL to 1000 ng/μL. This assay demonstrates robustness against common contaminants, including proteins, free nucleotides, salts, solvents, and detergents |
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| Smart-Q 100 for single sample processing | |
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| Smart-Q 800 for up to 8-sample processing | |
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| Accuris DNA Quantification Kits | |
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Ordering Information
| Smart-Q™ | Cat.No. | Products | Qty ( Pack ) | Order |
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BM-F1001 | Smart-Q 100 Fluorometer, single sample | 1 | |
| BM-F1008 | Smart-Q 800 Fluorometer, 8-channel1 | 1 | |
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BM-F0100 | Smart-Q Mini Fluorometer, 115V | 1 | |
| BM-F0100-E | Smart-Q Mini Fluorometer, 230V | 1 | |
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BM-F0100-TAB | Android tablet preloaded with Smart-Q Mini app | 1 | |
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BM-F1000-HS-100 | High Sensitivity dsDNA Quantification Kit, 100 assays | 1 Kit | |
| BM-F1000-HS-500 | High Sensitivity dsDNA Quantification Kit, 500 assays | 1 Kit | |
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| BM-F1000-HS1-100 | 1X High Sensitivity dsDNA Quantification Kit, 100 assays | 1 Kit | |
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| BM-F1000-HS1-500 | 1X High Sensitivity dsDNA Quantification Kit, 500 assays | 1 Kit | |
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| BM-F1000-BR-100 | Broad Range dsDNA Quantification Kit, 100 assays | 1 Kit | |
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| BM-F1000-BR-500 | Broad Range dsDNA Quantification Kit, 500 assays | 1 Kit | |
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| BM-F1000-BR1-100 | 1X Broad Range dsDNA Quantification Kit, 100 assays | 1 Kit | Current page | |
| BM-F1000-BR1-500 | 1X Broad Range dsDNA Quantification Kit, 500 assays | 1 Kit | |
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| BM-F1000-SD-100 | ssDNA Quantification Kit, 100 Assays | 1 Kit | |
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| BM-F1000-SD-500 | ssDNA Quantification Kit, 500 Assays | 1 Kit | |
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| BM-F1000-RHS-100 | High Sensitivity RNA Quantification Kit, 100 assays | 1 Kit | |
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| BM-F1000-RHS-500 | High Sensitivity RNA Quantification Kit, 500 assays | 1 Kit | |
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| BM-F1000-RBR-100 | Broad Range RNA Quantification Kit, 100 assays | 1 Kit | |
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| BM-F1000-RBR-500 | Broad Range RNA Quantification Kit, 500 assays | 1 Kit | |
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BM-F1001-T | 0.5mL Fluorescence Detection Tubes, pack of 250 | 250 | |
| BM-F1008-T | 0.2ml Smart-Q™ 800 Assay Tubes, pack of 1000 | 1000 | |
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| BM-F1008-T-8 | 0.2ml Smart-Q™ 800 Assay Tube Strips, pack of 120 | 120 | |
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