warning For Research Use Only (RUO). Not intended for clinical, diagnostic, or therapeutic use in humans.
Pack size
1000 mL
FNK-87-972
Buffers & Water — Phosphate Buffer
Dulbecco's Phosphate-Buffered Saline, PBS(-)
Dulbecco's Phosphate-Buffered Saline, PBS(-) (FNK-87-972) is a ready-to-use, calcium- and magnesium-free phosphate buffer supplied as a colorless, transparent liquid at pH 7.4–7.6 and 276–284 mOsm/kg H2O. Each lot is endotoxin, sterility, and cytotoxicity tested, and the buffer is prepared with ultrapure water for routine cell washing, reagent dissolution, and dilution work.
Calcium- and magnesium-free formulation, PBS(-)
pH 7.4–7.6 for stable, reproducible buffering
Osmolality 276–284 mOsm/kg H2O
Endotoxin tested, sterility tested (general bacteria and fungi not detected), and cytotoxicity tested
Prepared with ultrapure water of the grade used in serum-free culture media
Colorless and transparent; ready to use, no dilution required
Suitable for long-term storage at room temperature
Supplied as 1000 mL
SKU: FNK-87-972UNSPSC: 12161706Phosphate Buffer
Dulbecco's Phosphate-Buffered Saline, PBS(-) — 1000 mL
FormulationCalcium- and magnesium-free, PBS(-)
pH7.4-7.6
Osmolality276-284 mOsm/kg H2O
Ion contentNa+ 145, K+ 2, Cl- 139 mEq
AppearanceColorless, transparent liquid
EndotoxinTested
SterilityTested (bacteria and fungi not detected)
CytotoxicityTested
StorageRoom temperature
Size1000 mL
RUO
Why a defined PBS(-)
A fixed buffer background, lot to lot
PBS is the most-used reagent on most benches, which makes it an easy place for silent variability to enter an experiment. Laboratory-prepared or loosely specified PBS can drift in pH and osmolality between batches. FNK-87-972 is supplied ready to use at a defined pH and osmolality, with endotoxin, sterility, and cytotoxicity testing performed before release.
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Defined pH and osmolality
Released at pH 7.4–7.6 and 276–284 mOsm/kg H2O, so wash and dilution steps do not shift downstream assay conditions.
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Calcium- and magnesium-free
The PBS(-) formulation avoids introducing divalent cations during washing, dissociation, and adhesion-sensitive steps.
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Tested before release
Endotoxin, sterility, and cytotoxicity testing are performed on the product, with a Certificate of Analysis available on request.
visibility
Low optical background
Colorless and transparent, which keeps background low in imaging and absorbance-based readouts.
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Ready to use
Supplied as a working solution with nothing to weigh, dissolve, or pH-adjust, removing a common source of bench-to-bench variability.
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Room-temperature storage
Suitable for long-term storage at room temperature, so it does not consume cold-room or refrigerator space.
Applications
Where PBS(-) is used
FNK-87-972 supports routine cell washing, reagent dissolution and dilution, immunoassay workflows, and general laboratory and cellular medicine research.
Sample Preparation
Wash, Dilution & Reconstitution
Washing cells, dissolving reagents, and diluting stock solutions across general laboratory workflows.
WashDilutionReconstitution
Cell Culture
Rinse & Dissociation Steps
Calcium- and magnesium-free rinsing prior to enzymatic dissociation, and washing of adherent and suspension cultures.
AdherentSuspensionPrimary cells
Immunoassays
ELISA, Blotting & Staining
Wash and dilution buffer for ELISA, Western blot, immunohistochemistry, and immunofluorescence protocols.
ELISAWestern blotIHCIF
Research Use
Cellular Medicine Research & Animal Studies
General-purpose buffer for cellular medicine research and animal experiments conducted under Research Use Only conditions.
Cell therapy researchAnimal studies
Technical Specifications
Product specifications
Documented parameters for FNK-87-972 Dulbecco's PBS(-).
Physical & Chemical Parameters
Parameter
Specification
Formulation
Calcium- and magnesium-free PBS(-)
Appearance
Colorless, transparent liquid
pH
7.4-7.6
Osmolality
276-284 mOsm/kg H2O
Ion content
Na+ 145 mEq, K+ 2 mEq, Cl- 139 mEq
Testing
Parameter
Specification
Endotoxin
Tested
Sterility
Tested (general bacteria and fungi not detected)
Cytotoxicity
Tested
Water
Ultrapure water of the grade used in serum-free culture media
Storage & Handling
Parameter
Specification
Storage temperature
Room temperature
Storage stability
Suitable for long-term storage at room temperature
Format
Ready-to-use liquid buffer
Ordering & Documentation
Parameter
Specification
Size
1000 mL
UNSPSC
12161706
Intended use
Research Use Only (RUO); not for use in humans
This product is colorless and transparent. If turbidity, coloration, or insoluble residue is observed, discontinue use.
Formulation
Full composition
FNK-87-972 is manufactured to the fixed salt formulation and ion content below.
Component
CAS Number
Concentration
Purified Water
7732-18-5
—
Sodium Chloride
7647-14-5
8.00 g/L
Disodium Hydrogen Phosphate Dodecahydrate
10039-32-4
2.89 g/L
Potassium Dihydrogen Phosphate
7778-77-0
0.20 g/L
Potassium Chloride
7447-40-7
0.20 g/L
Ion
Concentration
Na+
145 mEq
K+
2 mEq
Cl-
139 mEq
Quality & Documentation
Lot testing and paperwork
Endotoxin
Endotoxin testing is performed on this product. A specific numeric limit is not stated in the available documentation.
Sterility
Tested for general bacteria and fungi, with none detected.
Cytotoxicity
Cytotoxicity testing is performed on this product.
Certificate of Analysis
A Certificate of Analysis is available on request. Contact Diagnocine Technical Service with your lot number.
Questions about this product, or need lot documentation? Contact support@diagnocine.com or call 551-245-1033.
FAQ
Frequently asked questions
Common questions about FNK-87-972 Dulbecco's PBS(-).
PBS(-) contains no calcium or magnesium. That matters wherever divalent cations would interfere — most commonly when rinsing adherent cells before enzymatic dissociation, since calcium and magnesium support the cell-adhesion interactions that the dissociation step is trying to break. PBS(+) includes both cations and is used where they are needed to maintain adhesion or function.
FNK-87-972 is specified at pH 7.4–7.6 with an osmolality of 276–284 mOsm/kg H2O and an ion content of Na+ 145 mEq, K+ 2 mEq, and Cl- 139 mEq. A measurement temperature for the pH specification is not stated in the available documentation.
No. This product is supplied as a ready-to-use liquid buffer and is used as received.
Store at room temperature. The buffer is suitable for long-term room-temperature storage. Because it is colorless and transparent by specification, any turbidity, coloration, or insoluble residue is a reason to discontinue use.
Endotoxin, sterility (general bacteria and fungi, none detected), and cytotoxicity testing are performed. A specific numeric endotoxin limit is not stated in the available documentation; request the Certificate of Analysis for lot-specific results.
FNK-87-972 is supplied as 1000 mL. A Certificate of Analysis is available on request — contact support@diagnocine.com with your lot number.
No. This product is labeled For Research Use Only (RUO) and is not intended for clinical, diagnostic, or therapeutic use in humans.
