Dulbecco's PBS without Ca2+and Mg2+ (D-PBS), Non-Sterile, 10X

FluxMPS™ Dulbecco's PBS without Ca²⁺ and Mg²⁺ (D-PBS), Non-Sterile, 10X

MPS-grade Dulbecco's phosphate-buffered saline concentrate (10X) formulated without calcium or magnesium, intended for organ-on-a-chip, microfluidic, and general cell culture workflows. Each lot is filtered twice through a 0.1 µm membrane in a clean environment and prepared with ASTM Type 1 ultrapure water to support a target pH of 7.4.

• 0.1 µm membrane filtration, performed twice (dual-pass), in a clean environment
• Target pH 7.4; customizable to 2X, 4X, 5X, or 10X concentration on request
• Free of Ca²⁺, Mg²⁺, protease, DNase, RNase, and endonuclease
• Prepared with ASTM Type 1 ultrapure water (0.05 µS/cm), deionized
• Non-sterile, Tissue Culture & Molecular Biology grade formulation
• Custom pH, concentration, and additive/detergent/inhibitor/protein formulations available on request

Engineered where standard buffers fail

Dulbecco's phosphate-buffered saline is a balanced salt solution designed for cell culture applications because of its osmotic balance property, similar to the natural condition of mammalian systems. D-PBS provides the appropriate milieu to briefly sustain the physiological and structural integrity (tonicity/viability) of cells in vitro. Balanced salts support viable conditions for cells only during experimental conditions outside their regular growth environment; when treated with D-PBS, cells maintain osmotic balance and physiological pH, and D-PBS without calcium and magnesium protects cells from acute fluctuations. Conventional 0.22 µm-filtered buffers can carry higher subvisible particulate loads and less consistent pH control, which is a concern for sensitive microchannel and imaging workflows.

Single-stage 0.1 µm membrane filtration, applied twice

Each lot of DCP-DPBSWOCAMG10X is processed through 0.1 µm membrane filtration, performed twice (2X) in sequence within a clean environment, prior to fill.

1. 1

   0.1 µm Membrane Filtration (Dual-Pass)

   All reagents are filtered twice (2X) through 0.1 micron membrane filters in a clean environment, reducing particulate load and helping to prevent mycoplasma contamination; the smallest known mycoplasma organisms measure approximately 0.2 microns.

Where D-PBS without Ca²⁺/Mg²⁺ is used

A wide range of uses include general cell culture, washing adhesion cells before dissociation, transporting cells or tissue samples, diluting cells for counting, and preparing reagents that provide inorganic salts essential for basic cell metabolism. D-PBS is widely used because its buffering capacity is effective at in vivo neutral pH across a range of applications. When included with calcium and magnesium, trypsinization/cell dissociation is less effective, but such inclusion is useful for transport media or reagent preparation; D-PBS without calcium and magnesium is generally used as a diluent or as an inorganic base for standard media preparation.

Automated Bioreactors & Robotics

For automated perfusion systems and robotic liquid handling, an optional 0.01 µm (10 nm) ultra-filtered variant is available to further protect sensitive valves, sensors, and microchannels.

• Total Particulate Exclusion — finer retention for closed-loop systems
• Valve & Sensor Protection — reduces particulate-driven fouling risk
• Extended Perfusion Stability — supports longer unattended run times

Inquiry Required: the 0.01 µm ultra-filtered grade is available on request; contact support@diagnocine.com.

Physical, chemical, and quality parameters

All values below reflect data stated for this specific formulation.

Full composition

Component concentrations are expressed as supplied at 10X strength.

Manufacturing & compliance

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.

How DCP-DPBSWOCAMG10X compares

A comparison against conventional 0.22 µm-filtered buffer.

Frequently asked questions

Common questions about DCP-DPBSWOCAMG10X.

Supporting literature

Curated references relevant to phosphate-buffered saline, buffer chemistry, and organ-on-a-chip applications.

1. Dulbecco R, Vogt M. Plaque formation and isolation of pure lines with poliomyelitis viruses. J Exp Med. 1954;99(2):167-182. doi:10.1084/jem.99.2.167
2. Good NE, et al. Hydrogen ion buffers for biological research. Biochemistry. 1966;5(2):467-477. doi:10.1021/bi00866a011
3. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32(8):760-772. doi:10.1038/nbt.2989
4. Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328(5986):1662-1668. doi:10.1126/science.1188302
5. Esch EW, et al. Organs-on-chips at the frontiers of drug discovery. Nat Rev Drug Discov. 2015;14(4):248-260. doi:10.1038/nrd4539
6. Zhang B, et al. Organ-on-a-chip devices advance to market. Lab Chip. 2017;17(14):2395-2420. doi:10.1039/C6LC01554A
7. Sung JH, et al. Recent advances in body-on-a-chip systems. Anal Chem. 2019;91(1):330-351. doi:10.1021/acs.analchem.8b05293
8. Low LA, et al. Organs-on-chips: into the next decade. Nat Rev Drug Discov. 2021;20(5):345-361. doi:10.1038/s41573-020-0079-3
9. Ingber DE. Human organs-on-chips for disease modelling, drug development and personalized medicine. Nat Rev Genet. 2022;23(8):467-491. doi:10.1038/s41576-022-00466-9