Dulbecco's PBS with Ca2+and Mg2+ (D-PBS), Non-Sterile, 10X

Cat. No.: DCP-DPBS10X


Size: Size:  500ml, 1000ml (1 Liter), 10 liter

pH 7.4  |  *Available other customization of pH, other customization of concentration (1X, 2X, 4X, 5X)  | Please Inquire
 
Storage: Room Temperature;  4°C recommended for a long term storage

Form & Appearance:  Sterilized Liquid, Clear, Colorless
 
Components:  26.7 mM Potassium Chloride (KCl: 2000.00 mg/L),  14.7 mM Potassium Phosphate monobasic (KH2PO4 : 2000 mg/L), 81.0 mM Sodium Phosphate Dibasic (Na2HPO4: 11500 mg/L), 81.0 mM Sodium Chloride (NaCl, 80000 mg/L),  9.0 mM Calcium Chloride (CaCl2, 1000 mg/L), 4.9 mM Magnesium Chloride Hexahydrate (MgCl2, 1000 mg/L)   

*Available for other customization & addition of chemicals/detergents/inhibitors/proteins/others  | Please Inquire

 
All Reagents were Sterilized by Filtering TWICE (2X) through 0.1 micron membrane filters in a clean environment.

Dulbecco's phosphate-buffered saline (DPBS) is a balanced salt solution designed for multiple cell culture applications due to its osmotic balance property similar to the natural condition of mammalian systems.  D-PBS is generally used to provide the appropriate milieu to sustain the physiological and structural integrity (i.e., tonicity/viability) of cells in vitro briefly.  Balanced Salts support viable conditions for cells only during experimental conditions outside of their regular growth environment. When treated with D-PBS, the cell maintains osmotic balance and physiological pH, and D-PBS protects the cells from acute fluctuations.
 
A wide range of uses include (a) general cell culture, (b) washing adhesion cells before dissociation, (c) transporting cells or tissue samples, (d) diluting cells for counting, and (e) preparing reagents for providing inorganic salts essential for basic cell metabolism. D-PBS is widely used because its buffering capacity is effective at in vivo neutral pH in various applications. When included with calcium and magnesium, trypsinization/ cell dissociation is less effective, but such inclusion is useful for transport media or for reagent preparation.  In addition, D-PBS is generally used as a diluent or as an inorganic base for standard media preparation. Deterioration of the D-PBS may be recognized by precipitate or particulate matter throughout the solution, cloudy appearance, color change, and/or pH change. 

All DiagnoCine Precision Sterile reagents are filtered-sterilized with a 0.1-micron filtration Twice (2X).  Thus, mycoplasma contamination is prevented.  The smallest size mycoplasma type can be about 0.2 microns.


Feature:

Ca (+), Mg (+)
Protease (-), DNAse (-), RNAse (-), Endonuclease (-)
Used ASTM 0.05 µS/cm Type 1 Ultrapure H2O | Deionized
Tissue Culture & Molecular Biology grade
 
RUO* | R&D use only | Not for human therapeutic use or human consumption
*RUO – Research Use Only


Intended use
*This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.

 * This product is manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision). All final packaging, quality assurance, and testing are done at the DiagnoCine R&D and Quality Testing Center.  All specific customization requests and assembly were accomplished at DiagnoCine Precision in Totowa, New Jersey, USA