Dulbecco's PBS with Ca2+and Mg2+ (D-PBS), Sterile, 1X
FluxMPS™ Dulbecco's PBS with Ca2+ and Mg2+ (D-PBS), Sterile, 1X
DCP-DPBS1X is an MPS-grade Dulbecco's phosphate-buffered saline formulated to pH 7.4 with calcium and magnesium for physiological osmotic balance. Filtered through 0.1 µm membrane filters twice (2X) in a clean environment, it is built for microchannel-safe handling in organ-on-a-chip (OoC), tissue-on-a-chip (ToC), and microphysiological system (MPS) workflows.
- Filtered through 0.1 µm membrane filters, twice (2X), in a clean environment
- pH 7.4, formulated with Ca2+ and Mg2+ for physiological osmotic balance
- ASTM Type 1 Ultrapure Water (0.05 µS/cm), deionized
- Protease-, DNase-, RNase-, and Endonuclease-free
- Manufactured under ISO 13485-certified, CE-approved facilities
- Microchannel-safe for organ-on-a-chip (OoC) and microfluidic workflows
- Customizable pH, concentration (2X, 4X, 5X, 10X), and additive content available on request
- pH7.4
- FormulationBalanced salt solution with Ca2+ and Mg2+
- Ionic ComponentsKCl, KH2PO4, Na2HPO4, NaCl, CaCl2, MgCl2
- Water QualityASTM Type 1 Ultrapure Water (0.05 µS/cm), Deionized
- Filtration0.1 µm membrane, twice (2X)
- SterilitySterile, filtered in a clean environment
- Nuclease StatusProtease-, DNase-, RNase-, Endonuclease-free
- StorageRoom Temperature; 4°C recommended long-term
- FormSterilized Liquid, Clear, Colorless
- Intended UseRUO - Research Use Only
Engineered where standard buffers fail
Conventional single-pass 0.22 µm-filtered buffers can carry finer particulates, mycoplasma-scale organisms, and pH/ionic drift into sensitive microchannel systems. DCP-DPBS1X is filtered through 0.1 µm membrane filters twice (2X) in a clean environment to reduce that risk before it reaches your chip or culture.
Microchannel-safe purity
Sequential 0.1 µm membrane filtration performed twice reduces particulates that could otherwise accumulate in narrow microfluidic channels.
Defined, physiological pH
Formulated to pH 7.4 with Ca2+ and Mg2+ to protect cells from acute osmotic and pH fluctuations outside their normal growth environment.
Ultrapure-grade water
Prepared with ASTM Type 1 Ultrapure Water (0.05 µS/cm), deionized, for a low-background aqueous matrix.
Low-background for imaging & assays
Nuclease-free (Protease-, DNase-, RNase-, Endonuclease-negative) formulation supports clean handling ahead of imaging, dissociation, or diluent-based workflows.
Defined, traceable composition
Characterized inorganic salts at stated molar concentrations, manufactured under ISO 13485-certified, CE-approved facilities.
Customization on demand
pH, concentration (2X, 4X, 5X, 10X), and addition of chemicals, detergents, inhibitors, proteins, or other components available on request.
Dual-pass 0.1 µm filtration system
All DCP-DPBS1X reagent is sterilized by filtering twice (2X) through 0.1 µm membrane filters in a clean environment, providing a repeated fine-filtration step ahead of fill.
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1
0.1 µm Pre-filtration
First pass through a 0.1 µm membrane filter removes larger particulates and aggregates before final fill.
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2
0.1 µm Sterile-filtration
Second pass through a 0.1 µm membrane filter, performed in a clean environment, is intended to help prevent mycoplasma contamination; the smallest mycoplasma type can be about 0.2 microns.
Performance vs. conventional buffer
Sequential dual-pass 0.1 µm membrane filtration in a clean environment is intended to retain fine particulates and mycoplasma-scale organisms more thoroughly than a single filtration pass.
© Diagnocine® - DCP-DPBS1X
Where DCP-DPBS1X fits your workflow
Dulbecco's phosphate-buffered saline with Ca2+ and Mg2+ maintains osmotic balance and physiological pH for cells briefly removed from their regular growth environment, supporting general cell culture, washing, transport, dilution, and reagent preparation.
Automated Bioreactors & Robotics
An optional 0.01 µm (10 nm) ultra-filtered variant is available on request for automated bioreactors and robotic liquid-handling platforms where extended unattended runtime is required.
- Total Particulate Exclusion for sensitive fluidic pathways
- Valve & Sensor Protection in automated perfusion systems
- Extended Perfusion Stability for long-duration runs
Inquiry Required: contact support@diagnocine.com to request the 0.01 µm grade.
Micro Physiological System (MPS) & Chip
Osmotic-balanced buffer for perfusion and rinse steps in chip-based culture systems.
Wash, Dilution & Reconstitution
Used to wash adhesion cells before dissociation, dilute cells for counting, and prepare reagents requiring inorganic salts for basic cell metabolism.
iPSC-Derived Model Handling
Supports briefly sustaining physiological tonicity and viability of sensitive iPSC-derived models outside their growth environment.
Endothelial & Primary Cell Perfusion
Ca2+/Mg2+ content supports transport-media and reagent-preparation uses where cell dissociation is not the goal.
ELISA, Blotting & Blocking
Serves as a diluent or inorganic base for standard media and reagent preparation in immunoassay workflows.
Microscopy & Optical Sensing
Clear, colorless formulation with low nuclease background supports live-cell rinse steps ahead of imaging.
DCP-DPBS1X specification sheet
All values below are drawn directly from this product's specification; a value not measured for this product is left blank rather than estimated.
| Parameter | Specification |
|---|---|
| Formulation | Balanced salt solution with potassium chloride, potassium phosphate monobasic, sodium phosphate dibasic, sodium chloride, calcium chloride, and magnesium chloride hexahydrate |
| Appearance | Sterilized Liquid, Clear, Colorless |
| pH | 7.4 |
| Osmotic Balance | Formulated to match physiological osmotic tonicity of mammalian systems |
| Ionic Composition | See the full composition table below |
| Ca2+ / Mg2+ Content | Included |
| Parameter | Specification |
|---|---|
| Sterility | Sterile, filtered through 0.1 µm membrane filters twice (2X) in a clean environment |
| Nuclease Status | Protease-negative, DNase-negative, RNase-negative, Endonuclease-negative |
| Water Quality ASTM | Type 1 Ultrapure Water (0.05 µS/cm), Deionized |
| Manufacturing Standard ISO | ISO 13485-certified, CE-approved facilities |
| Filtration System | 0.1 µm membrane filtration, twice (2X) |
| Grade | Tissue Culture & Molecular Biology grade |
| Parameter | Specification |
|---|---|
| Storage Temperature | Room Temperature |
| Long-Term Storage | 4 °C recommended |
| Deterioration Indicators | Precipitate or particulate matter, cloudy appearance, color change, and/or pH change |
| Form | Sterilized Liquid, Clear, Colorless |
| Parameter | Specification |
|---|---|
| Raw Material Grade | Tissue Culture & Molecular Biology grade |
| Manufacturing Facility ISO | ISO 13485-certified and CE-approved (Supplier of DiagnoCine Precision) |
| Final Assembly & QA | DiagnoCine R&D and Quality Testing Center; custom assembly at DiagnoCine Precision, Totowa, New Jersey, USA |
| Intended Use | Laboratory Research Use Only (RUO); not for human or animal therapeutic use, consumption, or diagnostic use |
Full composition
DCP-DPBS1X is a balanced salt formulation of characterized inorganic components at the molar and mass concentrations listed below.
| Component | CAS Number | Concentration |
|---|---|---|
| Potassium Chloride (KCl) | 7447-40-7 | 2.67 mM (200.00 mg/L) |
| Potassium Phosphate Monobasic (KH2PO4) | 7778-77-0 | 1.47 mM (200 mg/L) |
| Sodium Phosphate Dibasic (Na2HPO4) | 7558-79-4 | 8.10 mM (1150 mg/L) |
| Sodium Chloride (NaCl) | 7647-14-5 | 8.1 mM (8000 mg/L) |
| Calcium Chloride (CaCl2) | 10043-52-4 | 0.9 mM (100 mg/L) |
| Magnesium Chloride Hexahydrate (MgCl2·6H2O) | 7791-18-6 | 0.49 mM (100 mg/L) |
Manufacturing & compliance
DCP-DPBS1X is manufactured under ISO 13485-certified, CE-approved facilities, with final packaging, quality assurance, and testing performed at the DiagnoCine R&D and Quality Testing Center.
ISO 13485-Certified QMS
Manufactured under ISO 13485-certified, CE-approved facilities (Suppliers of DiagnoCine Precision).
Ultrapure Type 1 Water
Prepared with ASTM 0.05 µS/cm Type 1 Ultrapure Water, deionized.
Clean-Environment Filtration
Sterilized by filtering twice (2X) through 0.1 µm membrane filters in a clean environment.
Micro-Batch Precision
Specific customization requests and assembly are accomplished at DiagnoCine Precision in Totowa, New Jersey, USA.
Nuclease-Free Assurance
Protease-negative, DNase-negative, RNase-negative, and Endonuclease-negative formulation.
Sterility Assurance
Twice-filtered (2X) through 0.1 µm membrane filters in a clean environment.
Osmotic Balance
Designed with an osmotic balance property similar to the natural condition of mammalian systems.
Documentation / CoA
Contact support@diagnocine.com for lot-specific documentation.
How DCP-DPBS1X compares
A feature-level comparison against conventional single-pass, 0.22 µm-filtered D-PBS.
| Parameter | DCP-DPBS1X (FluxMPS™) | Conventional D-PBS (0.22 µm filtered) | Standard alternative (0.22 µm filtered) |
|---|---|---|---|
| Ca2+ / Mg2+ Inclusion | check_circle | cancel | cancel |
| 0.1 µm Filtration Passes | 2 | 0 | 0 |
| Water Quality | ASTM Type 1 Ultrapure (0.05 µS/cm) | Standard purified water | Standard purified water |
| Manufacturing QMS | check_circle ISO 13485 | cancel | cancel |
| Nuclease-Free Formulation | check_circle | cancel | cancel |
| Microfluidic Channel Compatibility | check_circle | cancel | cancel |
| Custom Formulation | check_circle | cancel | cancel |
Frequently asked questions
Common questions about DCP-DPBS1X formulation, filtration, and customization.
Supporting literature
Curated peer-reviewed literature relevant to phosphate-buffered saline use in cell culture and microphysiological systems.
- Dulbecco R, Vogt M. Plaque formation and isolation of pure lines with poliomyelitis viruses. J Exp Med. 1954;99:167-182. doi:10.1084/jem.99.2.167
- Huh D, Matthews BD, Mammoto A, Montoya-Zavala M, Hsin HY, Ingber DE. Reconstituting Organ-Level Lung Functions on a Chip. Science. 2010;328:1662-1668. doi:10.1126/science.1188302
- Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760-772. doi:10.1038/nbt.2989
- Low LA, Tagle DA. Tissue chips - innovative tools for drug development and disease modeling. Lab Chip. 2017;17:3026-3036. doi:10.1039/C7LC00462A
- Ingber DE. Human organs-on-chips for disease modeling, drug development and personalized medicine. Nat Rev Genet. 2022;23:467-491. doi:10.1038/s41576-022-00466-9
- Zhang B, Radisic M. Organ-on-a-chip devices advance to market. Lab Chip. 2017;17:2395-2420. doi:10.1039/C6LC01554A
- Uphoff CC, Drexler HG. Detection of mycoplasma contamination in cell cultures by PCR analysis. Methods Mol Biol/Leukemia. 2002. doi:10.1038/sj.leu.2402365
- van der Valk J, et al. Optimization of chemically defined cell culture media - replacing fetal bovine serum in mammalian in vitro methods. Toxicol In Vitro. 2010;24:1053-1063. doi:10.1016/j.tiv.2010.03.016