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DNA Markers (Low & High) Family

Product#: DNA-Markers_Low_High-Family
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Molecular-Size Markers / DNA (Low & High)

DNA Markers (DNA Ladders), Low & High Range

Size your PCR products, digests and genomic DNA against fragments of known length. Diagnocine DNA markers come ready-to-load in three ranges, from 100 bp to 15 kb, and every kit includes a 10X gel loading buffer and a TAE or TBE running-buffer concentrate.
For Research Use Only.

Find your marker See all 6 kits

Gel image of the 100 to 700 bp DNA marker, 2% TAE agarose gel stained with EB, 5 microL loaded
100–700 bp
Gel image of the 100 to 8000 bp DNA marker, 1% TAE agarose gel stained with EB, 5 microL loaded
100–8000 bp
Gel image of the 500 to 15,000 bp DNA marker, 0.8% TAE agarose gel stained with EB, 5 microL loaded
500–15,000 bp
3 ranges, 100 bp to 15 kb100–700 bp, 100–8000 bp and 500–15,000 bp ladders.
Ready-to-loadPremixed with loading buffer: load 5 µL straight into the well.
Complete kitMarker 500 µL, Loading Buffer [10X] 1 mL, TAE [50X] 500 mL or TBE [10X] 1000 mL.
What is a DNA marker? Uses Choose a kit Selection guide Formats TAE or TBE? Why Diagnocine FAQ

What is a DNA marker?

A DNA marker, also called a DNA ladder or DNA size standard, is a mixture of DNA fragments of known length. You load it in one lane of an agarose gel, next to your samples. After electrophoresis the marker forms a series of bands, and you compare your sample bands with it to estimate their size in base pairs (bp) or kilobases (kb).

1 · Load
Load the ladder in one lane and your samples in the lanes beside it.
2 · Run
DNA is negatively charged, so it migrates toward the positive electrode. Smaller fragments move faster and travel farther.
3 · Read
Stain the gel and compare each sample band with the ladder bands of known size.

A ladder with a defined amount of DNA in each band also supports approximate quantification: compare the brightness of a sample band with ladder bands of known mass. The appearance of the ladder bands also tells you whether the gel ran properly.

What are DNA markers used for?

PCR and RT-PCR verification

Confirm that an amplicon is the expected size.

Restriction digest analysis

Check fragment sizes after enzyme digestion and map plasmids.

Cloning checks

Verify inserts, linearized vectors and colony PCR products before cloning.

DNA integrity

Check the integrity of genomic and high-molecular-weight DNA.

Approximate quantification

Estimate how much DNA a band holds by comparing its intensity with ladder bands of known mass.

Teaching laboratories

Routine molecular biology training and teaching labs.

Choose a DNA Marker

Six kits: three size ranges, each with TAE or TBE running buffer. Filter by range or buffer, then open the product page.

Range
Buffer

Showing 6 of 6 DNA markers

100 bp1 kb15 kb

DNA Marker (100-700 bp) with TAE & Loading Dye

DCP-D100-700A · 7 bands · 2% agarose gel

TAE [50X] 500 mLReady-to-loadRef. band 500 bp
1 kit per unitView product
100 bp1 kb15 kb

DNA Marker (100-700 bp) with TBE & Loading Dye

DCP-D100-700B · 7 bands · 2% agarose gel

TBE [10X] 1000 mLReady-to-loadRef. band 500 bp
1 kit per unitView product
100 bp1 kb15 kb

DNA Marker (100-8000 bp) with TAE & Loading Dye

DCP-D100-8000A · 10 bands · 1% agarose gel

TAE [50X] 500 mLReady-to-loadRef. band 500 bp
1 kit per unitView product
100 bp1 kb15 kb

DNA Marker (100-8000 bp) with TBE & Loading Dye

DCP-D100-8000B · 10 bands · 1% agarose gel

TBE [10X] 1000 mLReady-to-loadRef. band 500 bp
1 kit per unitView product
100 bp1 kb15 kb

DNA Marker (500-15000 bp) with TAE & Loading Dye

DCP-D500-15000A · 9 bands · 0.8% agarose gel

TAE [50X] 500 mLReady-to-loadRef. band 2000 bp
1 kit per unitView product
100 bp1 kb15 kb

DNA Marker (500-15000 bp) with TBE & Loading Dye

DCP-D500-15000B · 9 bands · 0.8% agarose gel

TBE [10X] 1000 mLReady-to-loadRef. band 2000 bp
1 kit per unitView product

No marker matches both filters. Clear a filter to see more kits.

Which marker fits your fragments?

You are sizing Marker Bands (bp) Reference band Gel
PCR and RT-PCR products, colony PCR screens, small restriction fragments 100–700 bp 100, 200, 300, 400, 500, 600, 700 500 bp 2%
Mixed small and multi-kilobase work: amplicons, plasmid mapping, insert checks 100–8000 bp 100, 250, 500, 750, 1000, 1500, 2000, 3000, 5000, 8000 500 bp 1%
Long-range PCR, genomic and high-molecular-weight DNA, large inserts 500–15,000 bp 500, 1000, 1500, 2000, 3000, 5000, 7500, 10000, 15000 2000 bp 0.8%

Reference band: 100 ng DNA per 5 µL; every other band carries 50 ng per 5 µL. Gel = recommended agarose concentration for each marker, run at 4–10 V/cm.

Match the gel to the fragment size

Higher-percentage agarose gels separate small fragments better; lower-percentage gels separate large fragments better. Higher-percentage gels take longer to run, and lower-percentage gels tear more easily.

Agarose gel Effective separation range (linear DNA)
0.5% 2,000–50,000 bp
1.0% 400–8,000 bp
1.5% 200–3,000 bp
2.0% 100–2,000 bp

General guidance published for standard agarose gels. Fragments outside 100 bp–15 kb need a different ladder; contact support@diagnocine.com.

Ladder formats

Conventional

  • Supplied without loading dye.
  • You mix the ladder with loading buffer before loading.
  • Usually the most economical format.

Ready-to-load (Diagnocine)

  • Premixed with loading buffer, so there is no need to add a separate loading dye.
  • Pipette it straight into the well.
  • Saves a step and removes a common source of pipetting error.

Diagnocine DNA markers are ready-to-load. The Loading Buffer [10X] in each kit is for your samples: it adds density so samples sink into the wells and carries tracking dye to monitor migration.

TAE or TBE?

TAE [50X], 500 mL

  • Large fragments: TAE separates large DNA better; in TBE, bands above roughly 10 kb tend to compress.
  • Gel extraction and cloning: TAE contains no borate, which can inhibit enzymes such as ligase, so DNA cut from the gel performs well in ligation, cloning and sequencing.
  • Lower buffering capacity: replace the running buffer as needed on long runs.
  • Room-temperature stock; no cold storage needed.

TBE [10X], 1000 mL

  • Small fragments: sharper bands and better resolution of closely spaced fragments, especially under 1,000 bp.
  • Stable runs: greater buffering capacity than TAE; heats less, holds pH through long runs and tolerates higher voltages.
  • Not for DNA recovery: borate inhibits many enzymes, so TBE is not recommended when DNA will be recovered from the gel.
  • Room-temperature stock; no cold storage needed.

Why Diagnocine DNA markers?

Ready-to-load ladder

Premixed with loading buffer. Load 5 µL directly; no mixing, no extra pipetting.

Complete kit, one catalog number

DNA Marker, Loading Buffer [10X] for your samples, and a TAE or TBE concentrate for casting and running the gel.

Bright reference band

The 500 bp band (Low and Broad ladders) or 2000 bp band (High ladder) is enhanced for quick orientation, with a defined mass for approximate quantification.

Clear, easy-to-read bands

Well-resolved reference bands make size estimates quick and reliable.

ISO 13485-certified

Manufactured under ISO 13485-certified facilities. Diagnocine is an ISO 13485-certified life science company.

U.S.-based support and procurement

Based in Totowa, New Jersey: support@diagnocine.com, (201) 681-7263. Registered in SAM.gov and available through Jaggaer, SAP Ariba, ZAGENO, Labviva, VWR, eVA and Labscoop.

ALSO FROM DIAGNOCINE

FluxMPS™ buffers and cell culture media. Diagnocine’s own FluxMPS™ buffers and cell culture media are filtered through 0.1 µm membranes, and selected products also through 0.04 µm membranes. Both are finer than the 0.22 µm membranes used for conventional sterile filtration. Each FluxMPS™ product page states its filtration steps. The DNA marker kits on this page are not FluxMPS™ products.

Browse FluxMPS™ products

What is in every kit

Component Size Storage Shelf Life Shipping
DNA Marker (premixed with loading buffer) 500 µL -20°C 24 months Blue Ice
Loading Buffer [10X] 1 mL -20°C 12 months Blue Ice
TAE Buffer [50X] or TBE Buffer [10X] 500 mL / 1000 mL RT 24 months RT

Quality: Manufactured under ISO 13485-certified facilities. For Laboratory Use Only. Not for Clinical Use.

FAQ

DNA marker questions

Load 5 µL of the DNA marker into an agarose-gel well. Adjust the volume to the well width at approximately 1 µL per 1 mm of well width, and increase it proportionally for wider wells.
Run the 100–700 bp marker on a 2% gel, the 100–8000 bp marker on a 1% gel and the 500–15,000 bp marker on a 0.8% gel, at 4–10 V/cm. As a general rule, higher-percentage gels separate small fragments better and lower-percentage gels separate large fragments better.
Yes, approximately. Each Diagnocine marker has a defined amount of DNA per band: the reference band holds 100 ng and every other band 50 ng per 5 µL. Compare the brightness of your sample band with ladder bands of known mass.
Choose TAE when you will cut DNA out of the gel for ligation, cloning or sequencing, or when you need to resolve large fragments. Choose TBE for sharper bands of small, closely spaced fragments and for long or higher-voltage runs, when you do not need to recover the DNA.
Store the DNA Marker and the Loading Buffer [10X] at -20°C. After thawing is complete, store both at 4°C and avoid repeated freezing and thawing. Keep the TAE or TBE concentrate at room temperature. Shelf life: DNA Marker 24 months, Loading Buffer [10X] 12 months, running buffer 24 months.
Stain the gel with ethidium bromide (EB) or another suitable nucleic-acid stain, and visualize the DNA bands on a UV transilluminator.

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