RNA Marker 6000

RNA Marker 6000

Cat. No.: SB-G3372-25UL
Size: 25 uL


Description

RNA marker 6000 is a mixture of seven high-purified single-stranded RNA transcripts (in bases): 500 ,  1000 ,  2000 ,  3000 ,  4000 ,  5000 and 6000 bases, obtaining by in vitro transcription. The content of RNA in per microliter product is about 700 ng, which is suitable for routine gel electrophoresis of RNA.

Storage and Handling Conditions

Transport use wet ice. Store at 4 ℃ for periods up to 12 months. For longer periods, stored at -20 ℃ .


Assay Protocol

• Mix equal volumes of RNA Marker 6000 and 2×RNA Loading Buffer (about 1-2 μL), heat at 70 ºC for 10 minutes, and immediately ice-bath for 2 minutes. Next, electrophoretic detection can be carried out；
• RNA electrophoresis
  • A. Agarose gel electrophoresis (not recommended): 2-5% agarose gel (adding nucleic acid dye EB), electrophoresis with TAE Buffer;
  • B. 1-3% formaldehyde agarose denaturing gel electrophoresis (recommended): the preparation of 1% formaldehyde agarose gel as follows: stir 0.5 g agarose powder into 36 mL Rnase-Free Water (G4700 or G3004 is recommended) ；heat and melt the agarose, then add 5 mL 10×MOPS Buffer (G3373 is recommended). When the agarose solution cools to 60°C, add 9 mL 37% formaldehyde solution (add nucleic acid dye EB as required), mix thoroughly and pour the gel. Place the gel into an electrophoresis apparatus containing 1X MOPS buffer and observed by gel imager after completion.

• To avoid RNA degradation, use protective gloves and prepare fresh gels and electrophoresis buffers just before use. Plastic ware, tips and solutions should be treated with diethyl pyrocarbonate to prevent RNase contamination.
• RNA marker bands are single-stranded linear RNA, which is suitable for quantitative reference of single-stranded linear RNA samples. For total RNA, this product can only be used as a qualitative reference.
• To avoid repeated freeze/thaw degradation of RNA Marker, this product is recommended to aliquot reasonably.