Columbia Agar Base Plates/W Streptomycin Sulfate
Catalog No: DCP-CABPS
pH: 7.3 ± 0.2
Uses: Standard method base for enumeration and detection of bacteria in food and environmental samples, and clinical speciments
Size: 100mm
Concentration: 50 µg/mL
Storage: Keep away from light at 4°C
Shipping Condition: Blue ice
Apeearance: light amber, clear to slightly opalescent firm gel. Adding 5% sheep (or horse/rabbit) blood, the medium becomes opaque red
Sterile
Shelf Life: 4 weeks
*All agar plates are manufactured with ultrapure water, deionized and demineralized to minimize ionic and organic contaminants. The premium ISO certified agar solution is sterilized by a full cycle of autoclaving. Each 100mm plate has 20mL of agar medium in premium plastic dish
Description:
Columbia Agar Base with streptomycin sulfate is a nutritionally rich, non-blood agar medium in which standard Columbia agar base is supplemented with the aminoglycoside antibiotic streptomycin to make the medium selective by suppressing susceptible bacteria while allowing streptomycin-resistant organisms to grow. Columbia agar base is a complex peptone medium originally devised as a blood-agar base for the cultivation of fastidious organisms (e.g., streptococci, staphylococci, Neisseria, Haemophilus) as well as non-fastidious bacteria. It typically contains special peptones, yeast extract, maize/corn starch, sodium chloride, and agar at a near-neutral pH. The peptones and yeast extract provide amino acids, peptides, nitrogen, vitamins (including B-complex), and other growth factors that support luxuriant bacterial growth and good colony morphology. Maize starch serves as an energy source and helps neutralize toxic metabolites, aiding the recovery of more delicate organisms, while sodium chloride maintains osmotic balance and agar provides the solid support for colony development.
Streptomycin is an aminoglycoside antibiotic that binds to the 30S ribosomal subunit and interferes with protein synthesis, causing misreading of mRNA and ultimately bacterial cell death in susceptible organisms. When streptomycin sulfate is added to Columbia Agar Base, the medium becomes selective: many streptomycin-susceptible Gram-negative and some Gram-positive bacteria are inhibited, whereas strains that are intrinsically resistant or that carry streptomycin-resistance determinants can still grow.
* This product is manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision). All final packaging, quality assurance, and testing are done at the DiagnoCine R&D and Quality Testing Center. All specific customization requests and assembly were accomplished at DiagnoCine Precision in Totowa, New Jersey, USA
