Anti-MEKK3 Rabbit Polyclonal Antibody (WB)
Anti-MEKK3 Rabbit Polyclonal Antibody (WB)
General information
| Cat. No. | ：SB-GB111020 |
| Size | ：100 uL |
| Protein full name | ：Mitogen-activated protein kinase kinase kinase 3 |
| Synonym | ：MAPK/ERK kinase kinase 3, MEK kinase 3, MEKK 3, Map3k3, Mekk3 |
| Immunogen | ：Recombinant protein corresponding to Mouse MEKK3 |
| Isotype | ：IgG |
| Purity | ：Affinity purification |
| Predicted MW. | ：71KDa |
| Observed MW. | ：78KDa |
| Uniprot ID | ：Q99759, Q61084, B5DF98 |
| Long Term Storage | ：-20 ℃ or below |
| Shipping Condition | ：Dry Ice |
| Storage Buffer | ：PBS with 0.02% sodium azide,100 μg/ml BSA and 50% glycerol. |
Application
| Applications | Species | Dilution | Positive Tissue |
| WB | Human, Mouse, Rat | 1: 800-1: 1000 | liver, lung |
Description
The mitogen-activated protein/ERK kinase kinase 3 (MEKK3) is a member of a family of MEKK/Ste11 serine/threonine protein kinases. One function of MEKK3 is to serve as an upstream regulatory kinase in the mitogen activated protein kinase cascade, however, it also plays a critical role in other signaling pathways. MEKK3 regulates several important functions including cell survival and proliferation. It is ubiquitously expressed in multiple tissues and has been found in complexes with scaffold proteins and other kinases.Cerebral cavernous malformations (CCMs) complex regulation of MEKK3 is essential during vertebrate heart development. MEKK3 signalling and KLF2/4 may be targeted to develop new CCM therapeutics.
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Western blot analysis of mekk3 (GB111020) at dilution of 1: 800 |
Antigen Characterization
- Type: Primary
- Application: ELISA, Western Blot, Immunohistochemistry
- Species: Human, Mouse
- Recombinant: Yes
Human Diseases
- Esophageal Cancer: MEKK3 expression is significantly increased in esophageal dysplasia and esophageal squamous cell carcinoma (ESCC) .
- Inflammatory Diseases: Involved in the regulation of proinflammatory cytokines, contributing to conditions like inflammatory bowel disease .
- Neuroinflammation: Plays a critical role in neuroinflammation related to diseases such as Parkinson's disease
Cellular Signaling Pathways
- MAPK Pathway: MEKK3 is a crucial component that activates signaling cascades leading to cellular responses such as proliferation and inflammation .
- NF-κB Pathway: Regulates the expression of proinflammatory cytokines via IL-1R and TLR-mediated pathways .
- Hippo Pathway: MEKK3 interacts with components of this pathway to regulate cell growth and apoptosis
Aliases for MEKK3 Gene
References
- Fisher, Oriana S., et al. “Structure and Vascular Function of Mekk3–Cerebral Cavernous Malformations 2 Complex.” Nature News, Nature Publishing Group, 3 Aug. 2015, www.nature.com/articles/ncomms8937.
- Hasan, Raghibul, et al. “Mitogen Activated Protein Kinase Kinase Kinase 3 (MAP3K3/Mekk3) Overexpression Is an Early Event in Esophageal Tumorigenesis and Is a Predictor of Poor Disease Prognosis.” BMC Cancer, U.S. National Library of Medicine, 2 Jan. 2014, www.ncbi.nlm.nih.gov/pmc/articles/PMC3890584/.
- Cell Signaling Technology. “Mekk3 (D36G5) Rabbit Mab.” Cell Signaling Technology, www.cellsignal.com/products/primary-antibodies/mekk3-d36g5-rabbit-mab/5727.
- MEKK2 and MEKK3 Are Upregulated in MEKK1 Dwarf Plants ..., www.researchgate.net/figure/MEKK2-and-MEKK3-Are-Upregulated-in-mekk1-Dwarf-Plants-A-Wild-type-Col-0-and-mekk1_fig1_236929944.
- Mekk3 Antibody, Novus Biologicals - Antibodies, Primary Antibodies.” Fisher Scientific - Part of Thermo Fisher Scientific, www.fishersci.no/shop/products/mekk3-antibody-novus-biologicals-5/18106252.
