Anti-MT-ND5 Rabbit Polyclonal Antibody
Anti-MT-ND5 Rabbit Polyclonal Antibody
General information
| Cat. No. | ：SB-GB112639 |
| Size | ：100 uL |
| Protein full name | ：NADH-ubiquinone oxidoreductase chain 5 |
| Synonym | ：NADH dehydrogenase subunit 5, Mtnd5, mt-Nd5, Nd5 |
| Immunogen | ：KLH conjugated Synthetic peptide corresponding to Mouse MT-ND5 |
| Isotype | ：IgG |
| Purity | ：Affinity purification |
| Subcellular location | ：Mitochondrion |
| Predicted MW. | ：68 kDa |
| Observed MW. | ：68 kDa |
| Uniprot ID | ：P03921, P11661 |
| Long Term Storage | ：-20 ℃ or below |
| Shipping Condition | ：Dry Ice |
| Storage Buffer | ：PBS with 0.02% sodium azide,100 μg/ml BSA and 50% glycerol. |
Application
| Applications | Species | Dilution | Positive Tissue |
| WB | Mouse, Rat | 1: 1000-1: 2000 |
liver, brain |
| IF | Human, Rat | 1: 400-1: 800 |
lung, kidney, para-cancer lung |
Description
ND5 belongs to the complex I subunit 1 family. ND5 is a core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
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Western blot analysis of MT-ND5 (GB112639) at dilution of 1: 2000 Lane 1: Mouse liver tissue lysate Lane 2: Mouse brain tissue lysate Lane 3: Rat liver tissue lysate |
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Immunofluorescent analysis of paraformaldehyde-fixed human para-cancer lung using MT-ND5 (GB112639) at dilution of 1: 800
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Immunofluorescent analysis of paraformaldehyde-fixed rat lung using MT-ND5 (GB112639) at dilution of 1: 800 |
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Immunofluorescent analysis of paraformaldehyde-fixed rat kidney using MT-ND5 (GB112639) at dilution of 1: 800 |
Antigen Characterization
- Type: Primary
- Application: ELISA,Western Blot,IF
- Species: Human
- Recombinant: Yes
Human Diseases
- Leber Hereditary Optic Neuropathy (LHON): A genetic condition leading to vision loss due to mitochondrial dysfunction.
- Mitochondrial Myopathy: Disorders affecting muscle function due to impaired energy production.
- Leigh Syndrome: A severe neurological disorder characterized by progressive loss of mental and movement abilities.
- MELAS Syndrome: Mitochondrial Encephalomyopathy, Lactic Acidosis, and Stroke-like episodes
Cellular Signaling Pathways
- Respiratory Electron Transport Chain: Integral to ATP production through oxidative phosphorylation.
- Complex I Biogenesis: Involvement in the assembly and maintenance of mitochondrial complex I, crucial for cellular energy metabolism.
- NADH to Ubiquinone Activity: Facilitates electron transfer essential for metabolic processes.
Aliases for MT-ND5 Gene
- Mitochondrially Encoded NADH:Ubiquinone Oxidoreductase Core Subunit 5 2 5
- ND5 2 3 4 5
- MTND5 3 4 5
- NADH-Ubiquinone Oxidoreductase Chain 5 2 4
- NADH Dehydrogenase Subunit 5 3 4
- NAD5 2 5
- Mitochondrially Encoded NADH Dehydrogenase 5 2
- Complex I ND5 Subunit 2
- NADH Dehydrogenase 5 2
- EC 7.1.1.2 4
- EC 1.6.5.3 48
- NADH5 4
- MT-ND5 Mitochondrially Encoded NADH Dehydrogenase 5 [Homo Sapiens (Human)] - Gene - NCBI.” National Center for Biotechnology Information, U.S. National Library of Medicine, www.ncbi.nlm.nih.gov/gene?Cmd=DetailsSearch&Db=gene&Term=4540.
- MT-ND5 NADH Dehydrogenase 5, Mitochondrial [Mus Musculus (House Mouse)] - Gene - NCBI.” National Center for Biotechnology Information, U.S. National Library of Medicine, www.ncbi.nlm.nih.gov/gene?Cmd=DetailsSearch&Db=gene&Term=17721.
- MT-ND5 Mutation Exhibits Highly Variable Neurological Manifestations at Low Mutant Load - Ebiomedicine, www.thelancet.com/article/S2352-3964(18)30064-1/fulltext.
- Database, Rat Genome. MT-ND5 (NADH Dehydrogenase 5, Mitochondrial) - Rat Genome Database, rgd.mcw.edu/rgdweb/report/gene/main.html?id=736401.
- Pignataro, Daniela, et al. “A Missense MT-ND5 Mutation in Differentiated Parkinson Disease Cytoplasmic Hybrid Induces ROS-Dependent DNA Damage Response Amplified by Drosha.” Nature News, Nature Publishing Group, 25 Aug. 2017, www.nature.com/articles/s41598-017-09910-x.
