10% Neutral Buffered Formalin
FluxMPS™ 10% Neutral Buffered Formalin
FluxMPS™ 10% Neutral Buffered Formalin is an ultrapure, ready-to-use histology fixative buffered to pH 7.4 with approximately 4% formaldehyde. Processed through a quadruple-stage 0.1 µm and 0.04 µm membrane architecture and verified free of detectable DNase and RNase activity, it delivers gold-standard, artifact-free tissue preservation for diagnostic and research laboratories, including those handling organ-on-a-chip and microphysiological system specimens.
- Quadruple-stage filtration: 0.1 µm membrane (twice) and 0.04 µm membrane (twice)
- Neutral buffered to pH 7.4 for artifact-free tissue morphology
- Approximately 4% formaldehyde, ready-to-use formulation
- No detectable DNase or RNase activity
- Manufactured under ISO 13485-certified, CE-approved quality systems
- 24-month shelf life at room temperature
- Suitable for organ-on-a-chip and microphysiological system histological endpoint fixation
- Custom concentrations, additives, and pH available on request
- Formulation~4% Formaldehyde, Neutral Buffered
- pH7.4
- Filtration0.1 µm x2 + 0.04 µm x2
- DNase ActivityNone Detected
- RNase ActivityNone Detected
- AppearanceClear Solution
- StorageRoom Temperature, Protected from Light
- Shelf Life24 Months
- Fixative Ratio10:1 (Minimum)
- Fixation Time6-24 Hours
Engineered where standard fixatives fall short
Conventional 0.22 µm-filtered buffered formalin can carry subvisible particulates, unverified nuclease background, and inconsistent buffering that compromises nuclear detail and downstream molecular work. FluxMPS™ NBF is built to eliminate those failure modes.
Microchannel-Safe Purity
Quadruple-stage 0.1 µm and 0.04 µm membrane filtration removes particulate and aggregate contamination before final fill.
Precise, Stable pH
Buffered to pH 7.4 with a sodium phosphate monobasic/dibasic system that prevents autolysis and fixation artifacts.
Ultrapure-Grade Water
Formulated with Ultrapure Type 1 water (18.2 MΩ·cm), aligned with USP <85> quality practice.
Clean, Artifact-Free Preservation
Verified free of detectable DNase and RNase activity for reliable downstream molecular and IHC workflows.
Defined, Traceable Composition
A three-component buffered formalin formulation with lot-controlled ingredient concentrations.
Customization on Demand
Concentration, pH, and additive modifications available upon inquiry to support@diagnocine.com.
Quadruple-stage filtration system
10% Neutral Buffered Formalin is filtered 0.1-micron membrane twice and 0.04-micron membrane twice, combining redundant coarse pre-filtration with a final sub-0.1 µm polish for microchannel-safe, low-particulate fixative buffer.
- 1
0.1 µm Pre-filtration I
Large particulate and aggregate removal from the formaldehyde-based buffer, extending downstream filter life.
- 2
0.04 µm Pre-filtration II
Fine particulate and bioburden retention ahead of final processing.
- 3
0.1 µm Sterile-filtration I
Second-pass 0.1 µm redundancy prior to final polishing.
- 4
0.04 µm Sterile-filtration II — Final Polish
Ultimate 0.04 µm polish performed under ISO Class 5 (Class 100) conditions. This combination of two 0.1 µm passes and two 0.04 µm passes is designed to exclude contaminants, including mycoplasma — the smallest of which measure about 0.2 microns.
Performance vs. conventional buffer
Sequential 0.1 µm and 0.04 µm filtration passes remove finer particulates than a single conventional 0.22 µm filtration pass typically used for standard buffered formalin, reducing background interference in downstream molecular and imaging workflows.
© Diagnocine® — DCP-NBF4X
Where 10% Neutral Buffered Formalin is used
A ready-to-use, gold-standard fixative for histopathology laboratories worldwide, supporting both routine diagnostic work and advanced research applications.
Automated Bioreactors & Robotics
Automated tissue processors and robotic liquid handlers used in high-throughput histology laboratories benefit from ultra-clean, low-particulate fixative. An optional 0.01 µm (10 nm) ultra-filtered variant is available for the most sensitive automated systems.
- Total Particulate Exclusion for automated processing lines
- Valve & Sensor Protection in robotic liquid-handling systems
- Extended Perfusion Stability for continuous-fill processing equipment
Inquiry Required: please inquire if the 0.01 µm (10 nm) ultra-filtered variant, other concentrations, additional chemicals, or a different pH is needed — contact support@diagnocine.com.
Organ-on-a-Chip Tissue Fixation
Histological endpoint fixation of tissue harvested from microphysiological and organ-on-a-chip platforms.
Routine Diagnostic Tissue Fixation
Routine histological tissue fixation in diagnostic settings and preparation of samples for light microscopy.
IHC & Antigen Retrieval Workflows
Immunohistochemistry procedures with appropriate antigen retrieval.
Long-Term Museum Specimen Preservation
Permits restoration of natural color and long-term preservation of museum specimens.
Morphological Preservation for Research
Research applications requiring consistent morphological preservation of cellular constituents.
Tissue Processing & Embedding Prep
Standard fixative-to-tissue processing ahead of embedding and sectioning in high-throughput labs.
Detailed product specifications
Physical, chemical, purity, storage, and traceability parameters for DCP-NBF4X.
| Parameter | Specification |
|---|---|
| Formulation / Composition | 10% Neutral Buffered Formalin, approximately 4% formaldehyde |
| Appearance | Clear solution |
| pH USP <791> | 7.4 |
| Formaldehyde (40% stock) | 100 mL/L |
| Sodium phosphate, monobasic (NaH2PO4·H2O) | 4.0 g/L |
| Sodium phosphate, dibasic (Na2HPO4) | 6.5 g/L |
| Parameter | Specification |
|---|---|
| Sterility (Filtration-Based) | 0.1 µm membrane (twice) and 0.04 µm membrane (twice) |
| DNase Activity | None detected (18 hours, room temperature) |
| RNase Activity | None detected (18 hours, room temperature) |
| Water Quality | Ultrapure Type 1 water (18.2 MΩ·cm) |
| Fill Environment ISO Class 5 | ISO Class 5 (Class 100) |
| Manufacturing Standard ISO 13485 | ISO 13485-certified, CE-approved |
| Parameter | Specification |
|---|---|
| Storage Temperature | Room temperature, away from bright light |
| Shelf Life | 24 months |
| Fixative-to-Tissue Ratio | 10:1 (minimum) |
| Routine Fixation Time | 6-24 hours (larger specimens may require longer fixation times) |
| Parameter | Specification |
|---|---|
| Manufacturing QMS | ISO 13485-certified supplier facilities (DiagnoCine Precision) |
| Regulatory Alignment | CE-approved |
| Production & Customization Site | DiagnoCine R&D and Quality Testing Center, Totowa, New Jersey, USA |
| Intended Use | Research Use Only (RUO); histopathology, IHC, museum specimen preservation, research |
Full composition
10% Neutral Buffered Formalin is a defined, three-component buffered formalin formulation released per lot.
| Component | CAS Number | Concentration |
|---|---|---|
| Formaldehyde (40%) | 50-00-0 | 100 mL/L |
| Sodium phosphate, monobasic (NaH2PO4·H2O) | 10049-21-5 | 4.0 g/L |
| Sodium phosphate, dibasic (Na2HPO4) | 7558-79-4 | 6.5 g/L |
Manufacturing & compliance
Manufactured under ISO 13485-certified, CE-approved supplier facilities, with final packaging, QA, and customization completed at the DiagnoCine R&D and Quality Testing Center.
ISO 13485:2016 Quality System
Manufactured under ISO 13485-certified and CE-approved supplier facilities.
Ultrapure Type 1 Water
Formulated with 18.2 MΩ·cm Type 1 water to support a low-background fixative buffer.
Quadruple-Stage Filtration
0.1 µm membrane (twice) and 0.04 µm membrane (twice) processing performed under ISO Class 5 (Class 100) conditions.
Nuclease-Verified Micro-Batch QC
Each batch verified free of detectable DNase and RNase activity after 18-hour incubation at room temperature.
Sterility via Quadruple-Stage Filtration
0.1 µm membrane (twice) and 0.04 µm membrane (twice) filtration designed to exclude contaminants including mycoplasma.
CE-Approved Manufacturing
Produced under CE-approved supplier facilities alongside ISO 13485-certified quality systems.
Nuclease-Free Verification (DNase/RNase)
No DNase or RNase activity detected after 18-hour incubation with plasmid DNA or ribosomal RNA at room temperature.
Documentation / Certificate of Analysis
Lot-specific documentation covering composition, pH, filtration, and nuclease testing.
How DCP-NBF4X compares
FluxMPS™ DCP-NBF4X against conventional buffered formalin filtration architectures.
| Parameter | DCP-NBF4X (FluxMPS™) | Conventional Buffered Formalin | Standard Alternative |
|---|---|---|---|
| Filtration Architecture | 0.1 µm (twice) + 0.04 µm (twice) | Single 0.22 µm pass | Single 0.22 µm pass |
| Final Filtration Pore Size | 0.04 µm | 0.22 µm | 0.22 µm |
| Number of Filtration Stages | 4 | 1 | 1 |
| Neutral Buffered pH | check_circle 7.4 | cancel Variable | cancel Variable |
| Nuclease Testing (DNase/RNase) | check_circle | cancel | cancel |
| ISO 13485 / CE Manufacturing | check_circle | cancel | cancel |
| Custom Formulation Available | check_circle | cancel | cancel |
| Museum/Archival Specimen Suitability | check_circle | cancel | cancel |
Frequently asked questions
Common questions about DCP-NBF4X 10% Neutral Buffered Formalin.
Supporting literature
Curated references on formalin fixation chemistry, histology, and organ-on-a-chip workflows.
- Fox CH, Johnson FB, Whiting J, Roller PP. Formaldehyde fixation. J Histochem Cytochem. 1985. doi:10.1177/33.8.3894502
- Werner M, Chott A, Fabiano A, Battifora H. Effect of formalin tissue fixation and processing on immunohistochemistry. Am J Surg Pathol. 2000. doi:10.1097/00000478-200007000-00014
- Thavarajah R, Mudimbaimannar VK, Elizabeth J, Rao UK, Ranganathan K. Chemical and physical basics of routine formaldehyde fixation. J Oral Maxillofac Pathol. 2012. doi:10.4103/0973-029X.102496
- Srinivasan M, Sedmak D, Jewell S. Effect of fixatives and tissue processing on the content and integrity of nucleic acids. Am J Pathol. 2002. doi:10.1016/S0002-9440(10)64920-1
- Ingber DE. Reverse engineering human pathophysiology with organs-on-chips. Cell. 2016. doi:10.1016/j.cell.2016.05.048
- Low LA, Mummery C, Berridge BR, Austin CP, Tagle DA. Organs-on-chips: into the next decade. Nat Rev Drug Discov. 2021. doi:10.1038/s41573-020-0079-3
- Chatterjee S. Artefacts in histopathology. J Oral Maxillofac Pathol. 2014. doi:10.4103/0973-029X.141346
- Titford M. The long history of formaldehyde in histology and pathology. Biotech Histochem. 2009. doi:10.1080/10520290903039052
