Crystal Violet Staining Solution (Alcohol-Based)
Cat No.: DCP-CVA1X
Size: 500 mL
Sterile, Ultrapure, Filtered 0.1-micron membrane TWICE and 0.04-micron membrane ONCE.
Description:
Crystal Violet Staining Solution combines 1% crystal violet with a 10% ethanol/water solvent system, optimized for clinical laboratories and high-throughput research. This ready-to-use formulation adheres to CLSI standards for Gram staining (M07-A10) while enhancing dye penetration and cellular fixation.
Key Features:
- Enhanced Solubility: Ethanol-driven dissolution ensures 99.9% dye availability.
- Rapid Fixation: <30 sec cell fixation via ethanol-mediated dehydration.
- Stain Retention: 98% reduction in Gram-positive decolorization errors vs. aqueous formulations.
- Safety Optimized: Low-VOC formula with <10% ethanol.
Applications:
- Gram differentiation of Staphylococcus (purple) vs. E. coli (pink).
- Fungal hyphae visualization in histopathology.
- Biofilm quantification in 96-well plates.
Composition:
| Ingredients |
Concentration |
| Crystal Violet |
0.41% |
| Ethyl Alcohol |
7.36% |
| Isopropyl Alcohol |
0.41% |
| Methyl Alcohol |
0.37% |
| Water |
91.46% |
Safety Information:
- NFPA 704: Health 1, Flammability 2, Reactivity 0
- California Proposition 65 compliant (WARNING: Contains ethanol)
Quality Control:
- Appearance: Purple solution
- pH: 6.8-7.2
- Sterility: Filtered 0.1 micron Twice and 0.04 micron once.
- DNase activity: None was detected after incubation of plasmid DNA, and this product was used for 18 hours at room temperature.
- RNase activity: No RNase activity was detected after incubation of ribosomal RNA and this product for 18 hr at room temperature.
Storage and Shelf Life:
- Store at room temperature away from bright light.
- Shelf life is 24 months.
- Use before expiry date given on the product label.
CUSTOMIZATION: Please INQUIRE if other concentrations, additions of chemicals/compounds/proteins/supplements, different pH, and modifications are needed.
All DiagnoCine Precision Sterile buffers are filtered-sterilized with a 0.1-micron filtration two times and 0.04-micron filtration one time. Thus, mycoplasma contamination is prevented. The smallest size mycoplasma type can be about 0.2 microns.
* This product is manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision). All final packaging, quality assurance, and testing are done at the DiagnoCine R&D and Quality Testing Center. All specific customization requests and assembly were accomplished at DiagnoCine Precision in Totowa, New Jersey, USA.
