Crystal Violet Staining Solution (Alcohol-Based)
FluxMPS™ Crystal Violet Staining Solution (Alcohol-Based)
A ready-to-use, quadruple-stage filtered Crystal Violet Staining Solution combining 1% crystal violet dye chemistry with a 10% ethanol/water solvent system, formulated for rapid, CLSI M07-A10-aligned Gram differentiation. Sterile, ultrapure, and manufactured under ISO 13485-certified, CE-approved conditions for clinical laboratories, histopathology, and microfluidic biofilm quantification workflows.
- Quadruple-stage filtration — filtered 0.1 µm membrane twice and 0.04 µm membrane twice for microchannel-safe purity
- 0.41% crystal violet in an ethanol/water solvent system for rapid dye penetration and cellular fixation
- pH 6.8-7.2 for consistent, reproducible stain performance
- DNase- and RNase-free, verified after 18-hour incubation at room temperature
- 98% reduction in Gram-positive decolorization errors versus aqueous formulations
- Manufactured under ISO 13485-certified, CE-approved facilities in Totowa, New Jersey, USA
- Customizable concentration, pH, and additive content available on request
- Formulation0.41% Crystal Violet in ethanol/water solvent
- AppearancePurple solution
- pH6.8-7.2
- Filtration0.1 µm membrane twice + 0.04 µm membrane twice
- DNase ActivityNone detected (18 hr, RT)
- RNase ActivityNone detected (18 hr, RT)
- SterilityFiltered 0.1 µm + 0.04 µm membrane, quadruple-stage
- StorageRoom temperature, protected from light
- Shelf Life24 months
- Size500 mL
Engineered where standard stains fail
Conventional 0.22 µm-filtered or unfiltered crystal violet stains can carry subvisible particulate, inconsistent dye availability, and variable decolorization behavior. FluxMPS™ Crystal Violet Staining Solution addresses each failure mode with quadruple-stage filtration, a defined ethanol-driven solvent system, and lot-released composition.
Microchannel-safe purity
Final 0.04 µm filtration stage supports use in microfluidic biofilm assays and chip-based readouts where particulate matters.
Precise, consistent formulation
0.41% crystal violet in an ethanol-driven solvent system ensures 99.9% dye availability and CLSI M07-A10-aligned staining behavior.
Ultrapure-grade water
Formulated with Ultrapure Type 1 water (18.2 MΩ·cm), consistent with USP <85> water-quality expectations.
Low background for imaging & staining clarity
Rapid, uniform fixation and stain retention support clear Gram differentiation and biofilm quantification in 96-well plates.
Defined, traceable composition
Every solvent component — crystal violet, ethyl alcohol, isopropyl alcohol, methyl alcohol, water — is declared and lot-traceable.
Customization on demand
Alternate concentrations, pH targets, and chemical/compound additions available on request.
Quadruple-stage filtration system
Every DCP-CVA1X lot is filtered 0.1 µm membrane twice and 0.04 µm membrane twice, providing sequential particulate reduction beyond a single-pass 0.22 µm filtration and supporting sterility for microfluidic and clinical laboratory use.
-
1
0.1 µm Pre-filtration I
Removes large particulates and aggregates, extending the service life of downstream filters.
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2
0.04 µm Pre-filtration II
Retains fine particulates and bioburden ahead of final sterile filtration.
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3
0.1 µm Sterile-filtration I
Second-pass redundancy through a 0.1 µm membrane for consistent sterility assurance.
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4
0.04 µm Sterile-filtration II — Final Polish
Final 0.04 µm polish performed under ISO Class 5 (Class 100) aseptic fill conditions.
Performance vs. conventional stain
Sequential 0.1 µm and 0.04 µm filtration removes finer particulates than a single 0.22 µm pass typical of conventional crystal violet stains, supporting the product's 98% reduction in Gram-positive decolorization errors versus aqueous formulations.
© Diagnocine® — DCP-CVA1X
Applications
Designed for Gram differentiation, histopathology, and biofilm quantification workflows, with purity suited to microfluidic and chip-based readout systems.
Automated Bioreactors & Robotics
For automated staining lines, plate handlers, and robotic liquid systems, an optional 0.01 µm (10 nm) ultra-filtered variant of this staining solution is available to further protect fine-bore valves and sensors.
- Total Particulate Exclusion — minimizes subvisible particulate entering automated fluid paths
- Valve & Sensor Protection — reduces particulate load on fine-bore robotic components
- Extended Perfusion Stability — supports consistent performance in continuous-flow staining systems
Inquiry Required: Contact support@diagnocine.com to request the 0.01 µm ultra-filtered grade of this staining solution.
Micro Physiological System (MPS) & Chip
Endpoint crystal violet staining for biofilm and monolayer quantification within microfluidic chip channels.
Wash, Dilution & Reconstitution
Ready-to-use formulation requires no dilution or reconstitution prior to staining, per CLSI M07-A10 workflow.
iPSC-Derived Model Handling
Applicable as an endpoint stain for fixed iPSC-derived monolayer models requiring rapid cellular fixation.
Endothelial & Primary Cell Perfusion
Usable as an endpoint stain for quantifying perfused endothelial and primary cell monolayers.
ELISA, Blotting & Blocking
Supports crystal violet biofilm quantification assays in 96-well plate formats.
Microscopy & Optical Sensing
Used for Gram differentiation of Staphylococcus (purple) vs. E. coli (pink) and fungal hyphae visualization in histopathology.
Technical specifications
Measured and declared parameters for DCP-CVA1X, 500 mL.
| Parameter | Specification |
|---|---|
| Formulation / Composition | 1% crystal violet in a 10% ethanol/water solvent system |
| Appearance | Purple solution |
| pH (USP <791>) | 6.8-7.2 |
| Parameter | Specification |
|---|---|
| Sterility USP <71> | Filtered 0.1 µm membrane twice and 0.04 µm membrane twice |
| DNase Activity | None detected after 18 hr incubation with plasmid DNA at room temperature |
| RNase Activity | None detected after 18 hr incubation with ribosomal RNA at room temperature |
| Water Quality | Ultrapure Type 1 water (18.2 MΩ·cm) |
| Manufacturing Standard ISO 13485 | ISO 13485-certified, CE-approved facility |
| Fill Environment | ISO Class 5 (Class 100) aseptic fill |
| Parameter | Specification |
|---|---|
| Storage Temperature | Room temperature, away from bright light |
| Shelf Life | 24 months |
| Parameter | Specification |
|---|---|
| Manufacturing QMS | ISO 13485-certified, CE-approved (DiagnoCine Precision) |
| Regulatory Alignment | CLSI M07-A10; California Proposition 65 compliant (contains ethanol) |
| Production Method | Final packaging, QA, and testing at DiagnoCine R&D and Quality Testing Center, Totowa, New Jersey, USA |
| Intended Use | For Research Use Only (RUO) |
Full composition
Component concentrations expressed by weight percent, as released per manufacturing lot.
| Component | CAS Number | Concentration |
|---|---|---|
| Crystal Violet | 548-62-9 | 0.41% |
| Ethyl Alcohol | 64-17-5 | 7.36% |
| Isopropyl Alcohol | 67-63-0 | 0.41% |
| Methyl Alcohol | 67-56-1 | 0.37% |
| Water | 7732-18-5 | 91.46% |
Manufacturing & compliance
DCP-CVA1X is manufactured, filtered, and released under a controlled quality system.
ISO 13485:2016 QMS
Manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision).
Ultrapure Type 1 Water
Formulated with Ultrapure Type 1 water (18.2 MΩ·cm) as the solvent base.
ISO Class 5 Fill & Finish
Final quadruple-stage filtration polish performed under ISO Class 5 (Class 100) aseptic fill conditions.
Micro-Batch Precision
All packaging, quality assurance, and testing performed at the DiagnoCine R&D and Quality Testing Center in Totowa, New Jersey, USA.
DNase Activity
None detected after 18 hr incubation of plasmid DNA at room temperature.
RNase Activity
None detected after 18 hr incubation of ribosomal RNA at room temperature.
CLSI M07-A10 Alignment
Formulation adheres to CLSI standards for Gram staining (M07-A10).
Documentation / CoA
Lot-specific Certificate of Analysis available upon request.
How DCP-CVA1X compares
Comparing DCP-CVA1X against conventional and standard alternative crystal violet stains.
| Parameter | DCP-CVA1X (FluxMPS™) | Conventional 0.22 µm-filtered stain | Standard aqueous crystal violet |
|---|---|---|---|
| Solvent system | 1% crystal violet, 10% ethanol/water | Varies | Aqueous only |
| Final filtration pore size | 0.04 µm | 0.22 µm | Not specified |
| Number of filtration stages | 4 | 1 | Not specified |
| DNase / RNase-free verification | check_circle | cancel | cancel |
| CLSI M07-A10 alignment | check_circle | Varies | cancel |
| Stain retention vs. aqueous formulation | 98% reduction in Gram-positive decolorization errors | Not specified | Baseline |
| Manufacturing QMS | ISO 13485 / CE-approved | Unspecified | Unspecified |
| Custom formulation | check_circle | cancel | cancel |
Frequently asked questions
Answers to common questions about DCP-CVA1X.
Supporting literature
Curated references relevant to crystal violet Gram staining, microfluidic biofilm assays, and buffer/reagent filtration practice.
- Beveridge, T.J. Use of the Gram stain in microbiology. Biotech Histochem. 2001.doi:10.1080/10520290152034249
- Coico, R. Gram Staining. Curr Protoc Microbiol. 2005.doi:10.1002/9780471729259.mca03cs00
- O'Toole, G.A. Microtiter dish biofilm formation assay. J Vis Exp. 2011.doi:10.3791/2437
- Bukowski, M. et al. Biofilm formation and its role in Staphylococcus infections. Antibiotics. 2020.doi:10.3390/antibiotics9060312
- Bhatia, S.N., Ingber, D.E. Microfluidic organs-on-chips. Nat Biotechnol. 2014.doi:10.1038/nbt.2989
- Halldorsson, S. et al. Advantages and challenges of microfluidic cell culture in polydimethylsiloxane devices. Biosens Bioelectron. 2015.doi:10.1016/j.bios.2014.07.029
- Azeredo, J. et al. Critical review on biofilm methods. Crit Rev Microbiol. 2017.doi:10.1080/1040841X.2016.1208146
- Cappuccino, J.G., Welsh, C. Microbiology: A Laboratory Manual. 12th ed. Pearson, 2020.doi:10.1604/9780134806677
