DCP-CVW1X is an MPS-grade, water-based 1% crystal violet stain formulated in Ultrapure Type I water (18.2 MΩ·cm). Filtered 0.22-micron membrane once, this zero-alcohol formulation avoids solvent-induced cell shrinkage and is built for microfluidic-channel-safe staining workflows.
Filtered 0.22-micron membrane once.
Zero-alcohol formulation: ≤2% dimensional change vs. 15% in ethanol-based stains
QC-released at pH 6.8-7.2 for reproducible counterstaining
Formulated in Ultrapure Type I water (18.2 MΩ·cm)
DNase- and RNase-activity tested: none detected after 18 hr incubation at room temperature
Compatible with methanol, formaldehyde, or cryopreservation fixation methods
Custom concentration, pH, and additive content available on request
SKU: DCP-CVW1XUNSPSC: 12161500 · Indicators and reagents Staining Solution
Crystal Violet Staining Solution (Water-Based), 500 mL
Conventional alcohol-based crystal violet stains carry solvent-induced cell shrinkage, single-pass 0.22 µm filtration, and undocumented nuclease activity. DCP-CVW1X is built for microfluidic-channel-safe, low-background staining workflows.
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Microchannel-safe purity
filtration ( 0.22 µm membrane once) removes fine particulates before final packaging, supporting use in narrow microfluidic channels without clogging risk.
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Precise, stable pH
QC-released at pH 6.8-7.2, giving reproducible counterstaining intensity across replicate CFU and colony-counting assays.
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Ultrapure-grade water
Formulated in Type I ultrapure water (18.2 MΩ·cm), minimizing trace ionic and organic background in the final stain.
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Low background for imaging & assays
Zero-alcohol base limits dimensional change to ≤2% versus 15% for ethanol-based stains, preserving cell and colony morphology for live/dead imaging in microfluidic devices.
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Defined, traceable composition
Simple two-component formulation (1% Crystal Violet, 99% ultrapure water) with DNase- and RNase-activity verified after 18 hr incubation at room temperature.
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Customization on demand
Alternate concentrations, added chemicals/compounds/proteins/supplements, and modified pH are available on request.
Applications
Where DCP-CVW1X is used
A zero-alcohol, quadruple-stage-filtered crystal violet stain suited to colony counting, live/dead discrimination, nucleic acid counterstaining, and general laboratory demonstrations.
Automated Bioreactors & Robotics
Next-Generation System Uptime
For automated staining stations and robotic liquid handlers, an optional 0.01 µm (10 nm) ultra-filtered variant of this stain can be produced on request to further protect valves and sensors in continuous-run systems.
Total Particulate Exclusion: reduces sub-visible particulate load beyond the standard quadruple-stage architecture
Two-component, lot-controlled formulation released per DiagnoCine QC.
Component
CAS Number
Concentration
Crystal Violet
548-62-9
1%
Water
7732-18-5
99%
Customization: please inquire if other concentrations, additions of chemicals/compounds/proteins/supplements, different pH, or other modifications are needed — support@diagnocine.com.
Quality Assurance
Manufacturing & compliance
Manufactured under ISO 13485-certified, CE-approved facilities, with final packaging, QA, and testing at the DiagnoCine R&D and Quality Testing Center.
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ISO 13485:2016 QMS
Manufactured under an ISO 13485-certified, CE-approved quality management system.
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Ultrapure Type 1 Water
Formulated in 18.2 MΩ·cm Type I water to minimize background.
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Micro-Batch Precision
Customization and assembly for specific requests are accomplished at DiagnoCine Precision in Totowa, New Jersey, USA.
DNase Activity
None detected after incubation of plasmid DNA with this product for 18 hours at room temperature.
RNase Activity
No RNase activity detected after incubation of ribosomal RNA with this product for 18 hours at room temperature.
Water Quality
Formulated in Ultrapure Type I water, 18.2 MΩ·cm.
Documentation / CoA
A Certificate of Analysis documenting appearance, pH, sterility, and DNase/RNase testing is available on request.
Common questions about DCP-CVW1X Crystal Violet Staining Solution (Water-Based).
Yes. Its zero-alcohol formulation and quadruple-stage filtration (0.1 µm membrane twice, 0.04 µm membrane twice) avoid solvent-induced cell shrinkage and particulate accumulation, supporting live/dead discrimination in microfluidic devices and other OoC/MPS workflows.
DCP-CVW1X is filtered 0.1-micron membrane twice and 0.04-micron membrane twice, four sequential passes rather than a single 0.22 µm pass, removing finer particulates and reducing bioburden and mycoplasma risk before final packaging.
The solution is QC-released at pH 6.8-7.2 with 1% w/v crystal violet. Alternate concentrations, pH, and additive formulations are available on request; contact support@diagnocine.com.
The product description does not specify a measurement temperature for the pH reading. The product is stored at 2-8°C, away from bright light, with a 24-month shelf life.
Yes. DiagnoCine can prepare custom versions with alternate concentrations, added chemicals/compounds/proteins/supplements, or modified pH on request; contact support@diagnocine.com.
An endotoxin specification is not part of the standard release panel for this stain. Instead, DNase and RNase activity are verified, with none detected after 18-hour incubation at room temperature.
Yes, a CoA is available on request, documenting appearance, pH, sterility/filtration method, and DNase/RNase activity testing. Email support@diagnocine.com.
Scientific References
Supporting literature
Curated references relevant to crystal violet staining, colony-forming assays, and microfluidic/organ-on-a-chip applications.
Bhattacharya, S. et al. Organs-on-chips: a fast track for engineered human tissues in drug development. Cell Stem Cell. doi:10.1016/j.stem.2018.02.011
Feoktistova, M. et al. Crystal violet assay for determining viability of cultured cells. Cold Spring Harb Protoc. doi:10.1101/pdb.prot087379
Ingber, D.E. Human organs-on-chips for disease modelling, drug development and personalized medicine. Nat Rev Genet. doi:10.1038/s41576-022-00466-9
Zink, D. et al. Assessment of nephrotoxicity in an organ-on-a-chip system. Toxicol Res. doi:10.1093/toxres/tfaa009
Sambrook, J. & Russell, D.W. Detection of DNA in agarose gels. Molecular Cloning: A Laboratory Manual. doi:10.1101/pdb.top403
Halldorsson, S. et al. Advantages and challenges of microfluidic cell culture in polydimethylsiloxane devices. Biosens Bioelectron. doi:10.1016/j.bios.2014.07.029
