UltraClean PVSA-Sodium Phosphate, 1X
FluxMPS™ UltraClean PVSA-Sodium Phosphate, 1X
An MPS-grade, RNase-safe 1X sodium phosphate buffer supplemented with polyvinylsulfonic acid (PVSA), a potent polymeric RNase inhibitor, to help preserve RNA integrity during handling, processing, and short-term storage. Formulated at a stable, near-neutral pH and quadruple-stage filtered for microchannel-safe purity across molecular biology and microfluidic workflows.
- Quadruple-stage filtration: 0.1 µm membrane twice and 0.04 µm membrane twice for microchannel-safe, RNase-safe purity
- Supplemented with PVSA (1 g/L), a potent polymeric RNase inhibitor for extra RNA protection
- Ready-to-use 1X sodium phosphate buffering system at pH 7.4
- Formulated with ultrapure, nuclease-free Type 1 water (18.2 MΩ·cm)
- Sterile, colorless, particulate-free solution supplied at working strength
- Manufactured under ISO 13485-certified, CE-approved facilities
- Suited to microfluidic, organ-on-a-chip (OoC), and MPS-grade nucleic acid workflows
- Custom pH, concentration, and additive formulations available on request
- Catalog No.DCP-PHOSPVSA1X
- pH7.4
- Formulation8 mM Na2HPO4 + 2 mM NaH2PO4 (1X)
- RNase ProtectionPVSA, 1 g/L
- Filtration0.1 µm x2 + 0.04 µm x2
- Water QualityUltrapure, nuclease-free
- SterilitySterile
- Storage2–8°C, do not freeze
- Shelf Life12 months
- Sizes Available500 mL, 1000 mL
Engineered where standard buffers fail
Conventional 0.22 µm-filtered phosphate buffers can carry subvisible particulates, adventitious nucleases, and inconsistent pH that compromise RNA integrity and microchannel performance. FluxMPS™ UltraClean PVSA-Sodium Phosphate, 1X is built to close those gaps.
Microchannel-Safe Purity
Quadruple-stage filtration through 0.1 µm and 0.04 µm membranes, each applied twice, minimizes particulates that can clog or foul microfluidic channels.
Precise, Stable pH
A defined 1X sodium phosphate system (8 mM Na2HPO4 + 2 mM NaH2PO4) holds pH 7.4 for consistent nucleic acid handling.
Ultrapure-Grade Water
Formulated with ultrapure, nuclease-free Type 1 water (18.2 MΩ·cm) to keep adventitious nucleases and contaminants out of sensitive RNA workflows.
RNase-Safe RNA Protection
PVSA (1 g/L) provides robust inhibition across a broad spectrum of RNases, adding a layer of protection beyond standard nuclease-free handling practices.
Defined, Traceable Composition
Manufactured from molecular biology-grade sodium phosphate salts and PVSA under controlled, lot-level manufacturing.
Customization on Demand
Alternate concentrations, pH, and added chemicals, compounds, proteins, or supplements are available — contact support@diagnocine.com.
Quadruple-stage filtration system
Every lot of UltraClean PVSA-Sodium Phosphate, 1X passes through 0.1 µm membrane filtration twice and 0.04 µm membrane filtration twice, layering redundant purification stages ahead of aseptic fill.
- 1
0.1 µm Pre-Filtration I
Removes large particulates and aggregates, extending the life of downstream filters.
- 2
0.04 µm Pre-Filtration II
Retains fine particulates and bioburden ahead of the second filtration pass.
- 3
0.1 µm Sterile-Filtration I
A second 0.1 µm pass provides redundant particulate and bioburden reduction.
- 4
0.04 µm Sterile-Filtration II — Final Polish
A second 0.04 µm pass delivers the final polish ahead of aseptic fill.
Performance vs. conventional buffer
Sequential 0.1 µm and 0.04 µm filtration, each applied twice, removes finer particulates than a single-pass 0.22 µm filtration typically used in standard phosphate buffers.
© Diagnocine® — DCP-PHOSPVSA1X
Where UltraClean PVSA-Sodium Phosphate, 1X is used
A ready-to-use, RNase-safe 1X sodium phosphate buffer suited to nucleic acid handling, cell and tissue processing, and molecular biology workflows where phosphate buffer and RNA protection are preferred.
Automated Bioreactors & Robotics
For automated perfusion and robotic liquid-handling platforms, an optional 0.01 µm (10 nm) ultra-filtered variant of this buffer is available on request.
- Total Particulate Exclusion for automated fluidic paths
- Valve & Sensor Protection in closed-loop perfusion systems
- Extended Perfusion Stability across long-duration runs
Inquiry Required: contact support@diagnocine.com to request the 0.01 µm ultra-filtered grade.
Micro Physiological System (MPS) & Chip
A defined-pH, RNase-safe phosphate buffer for nucleic acid handling within microfluidic and chip-based systems.
RNA/DNA Resuspension & Dilution
Resuspension and dilution of purified nucleic acids in a phosphate-based buffer with added RNase protection for storage or downstream analysis.
Short-Term RNA Storage
Temporary storage of RNA-containing samples, lysates, or purified RNA in a controlled, near-neutral phosphate buffer.
Gentle Cell & Tissue Washing
Washing or processing of cells and tissues in workflows where RNA integrity is important and phosphate buffer is desired.
Sample Prep for RT, qPCR & Library Prep
Handling and transfer of nucleic acids prior to downstream enzymatic applications, as compatible with enzyme-specific phosphate tolerance.
General 1X Phosphate Procedures
Any procedure requiring 1X sodium phosphate with enhanced RNA protection under defined pH and ionic conditions.
Specifications at a glance
Measured and declared values for UltraClean PVSA-Sodium Phosphate, 1X (DCP-PHOSPVSA1X).
| Parameter | Specification |
|---|---|
| Formulation | 1X sodium phosphate: 8 mM Na2HPO4 + 2 mM NaH2PO4 |
| Appearance | Colorless, clear solution; free of visible particulates |
| pH USP <791> | 7.4 |
| RNase Protection | PVSA (Polyvinylsulfonic Acid), 1 g/L |
| Parameter | Specification |
|---|---|
| Sterility | Sterile |
| Filtration Quadruple-stage | 0.1 µm membrane twice + 0.04 µm membrane twice |
| Water Purity | Ultrapure, nuclease-free Type 1 water (18.2 MΩ·cm) |
| Manufacturing Std. | ISO 13485-certified, CE-approved facilities |
| Parameter | Specification |
|---|---|
| Storage Temperature | 2–8°C |
| Freeze-Thaw | Do not freeze |
| Shelf Life | 12 months |
| Parameter | Specification |
|---|---|
| Raw Material Grade | Molecular biology-grade sodium phosphate salts and PVSA |
| Manufacturing QMS | ISO 13485-certified (Suppliers of DiagnoCine Precision) |
| Regulatory Alignment | CE-approved facilities |
| Production Method | Final packaging, QA, and testing at the DiagnoCine R&D and Quality Testing Center; customization and assembly at DiagnoCine Precision, Totowa, New Jersey, USA |
| Intended Use | Research Use Only (RUO) |
Full composition
1X sodium phosphate buffer supplemented with PVSA, prepared in ultrapure, nuclease-free water.
| Component | CAS Number | Concentration |
|---|---|---|
| Disodium Hydrogen Phosphate | 7558-79-4 | 8 mM |
| Sodium Dihydrogen Phosphate | 7558-80-7 | 2 mM |
| PVSA (Polyvinylsulfonic Acid) | 25213-24-5 | 1 g/L |
| Water (Ultrapure, nuclease-free) | 7732-18-5 | To volume |
Manufacturing & compliance
Manufactured under ISO 13485-certified and CE-approved facilities, with final packaging, quality assurance, and testing performed at the DiagnoCine R&D and Quality Testing Center.
ISO 13485:2016 QMS
Manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision).
Ultrapure Type 1 Water
Formulated with ultrapure, nuclease-free water to protect RNA integrity.
Quadruple-Stage Filtration
0.1 µm membrane twice and 0.04 µm membrane twice for microchannel-safe, RNase-safe purity.
Micro-Batch Precision
Customization and assembly accomplished at DiagnoCine Precision in Totowa, New Jersey, USA.
Endotoxin USP <85> BET
Formulated using low-endotoxin raw materials and bacterial endotoxin test methodology.
Particulate Matter USP <788> Method 2
Quadruple-stage filtration is validated against USP <788> Method 2 light-obscuration methodology.
Osmolality USP <785>
Formulated as a defined 1X phosphate system consistent with USP <785> osmolality methodology.
Documentation / CoA
A Certificate of Analysis is available — contact support@diagnocine.com.
How DCP-PHOSPVSA1X compares
UltraClean PVSA-Sodium Phosphate, 1X against conventional 0.22 µm-filtered phosphate buffers.
| Parameter | DCP-PHOSPVSA1X (FluxMPS™) | Conventional Buffer | Standard Alternative |
|---|---|---|---|
| RNase Protection (PVSA) | check_circle | cancel | cancel |
| Final Filtration Pore Size | 0.04 µm | 0.22 µm | 0.22 µm |
| Number of Filtration Stages | 4 | 1 | 1 |
| Sterile Solution | check_circle | check_circle | cancel |
| Water Quality | Ultrapure Type 1 | Purified | Purified |
| Manufacturing QMS | check_circle ISO 13485 | cancel | cancel |
| Microfluidic/OoC Channel Compatibility | check_circle | cancel | cancel |
| Defined pH (7.4) | check_circle | check_circle | cancel |
| Custom Formulation Available | check_circle | cancel | cancel |
Frequently asked questions
Common questions about UltraClean PVSA-Sodium Phosphate, 1X.
Supporting literature
Curated literature relevant to phosphate buffering, RNase inhibition, and microfluidic/organ-on-a-chip applications.
- Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014. doi:10.1038/nbt.2989
- Wilson KS. Ribonuclease inhibitors in nucleic acid research. Methods Enzymol. doi:10.1016/S0076-6879(01)41153-3
- Good NE, et al. Hydrogen ion buffers for biological research. Biochemistry. 1966. doi:10.1021/bi00866a011
- Blow N. Sample preparation: the pre-emptive strike. Nat Methods. 2007. doi:10.1038/nmeth1030
- Farrell RE. RNA Methodologies: A Laboratory Guide for Isolation and Characterization. doi:10.1016/C2009-0-64180-5
- Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010. doi:10.1126/science.1188302
- USP General Chapter <85> Bacterial Endotoxins Test. doi:10.31003/USPNF_M99035_02_01
- USP General Chapter <788> Particulate Matter in Injections. doi:10.31003/USPNF_M99725_02_01
