Tris-Glycine SDS Buffer [1X]
FluxMPS™ Tris-Glycine SDS Buffer [1X]
An MPS-grade, quadruple-stage ultra-filtered 1X Tris-Glycine SDS running buffer built for reproducible SDS-PAGE, western blotting, and protein denaturation workflows. Formulated at 25 mM Tris base, 192 mM glycine, and 0.1% SDS with a controlled pH of 8.3 (± 0.05), it is sterile-filtered to remove particulates that interfere with electrophoresis, microfluidic electrophoresis chips, and low-background imaging assays. Diagnocine calls it “the world’s cleanest buffer for all cell and molecular biology experiments.”
- Quadruple-stage filtered: 0.1 µm membrane twice and 0.04 µm membrane twice
- Defined 1X formulation: 25 mM Tris base, 192 mM glycine, 0.1% SDS
- Controlled pH 8.3 (± 0.05) for consistent electrophoretic separation
- DNase- and RNase-tested for molecular biology-grade purity
- Sterile-filtered in a controlled environment, ready to use out of the bottle
- Custom concentrations, pH, and chemical/protein/supplement additions available on request
- pH8.3 (± 0.05)
- Tris base / Glycine25 mM / 192 mM
- SDS Concentration0.1%
- SterilityFiltered 0.1 µm twice, sterile environment
- DNase ActivityNone detected
- RNase ActivityNone detected
- Filtration0.1 µm x2 + 0.04 µm x2
- StorageRoom temperature
- Shelf Life2 years
- AppearanceClear, colorless liquid
Engineered where standard running buffer falls short
Conventional 0.22 µm-filtered running buffer can carry subvisible particulates and bioburden that accumulate in microfluidic electrophoresis channels, contribute to smearing or background noise in sensitive imaging assays, and introduce lot-to-lot pH or ionic drift. FluxMPS™ Tris-Glycine SDS Buffer [1X] is built to remove those failure modes at the source.
Microchannel-safe purity
Final 0.04 µm filtration stage reduces particulates that would otherwise clog microfluidic electrophoresis channels and chip-based separation platforms.
Precise, stable pH
Formulated to pH 8.3 (± 0.05) with defined Tris and glycine molarity so current flow and protein migration stay reproducible run to run.
Ultrapure-grade water
Prepared with ultrapure Type 1 water (18.2 MΩ·cm) as the base to minimize trace contaminants in every batch.
Low background for imaging & assays
Sterile filtration and DNase/RNase testing help keep western blot chemiluminescence and fluorescence detection free of background artifacts.
Defined, traceable composition
Every lot is formulated to the same 25 mM Tris base, 192 mM glycine, and 0.1% SDS specification with documented quality control.
Customization on demand
Alternate concentrations, pH, or added chemicals, compounds, proteins, and supplements are available — contact support@diagnocine.com.
Quadruple-stage filtration system
Every batch of Tris-Glycine SDS Buffer [1X] is filtered 0.1 µm membrane twice and 0.04 µm membrane twice in a controlled, sterile environment — sequential large-particulate removal followed by fine-particulate and bioburden retention on each pass.
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1
0.1 µm Pre-filtration I
First-pass removal of large particulates and aggregates, extending the life of downstream filters.
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2
0.04 µm Pre-filtration II
Fine-particulate and bioburden retention, helping prevent mycoplasma contamination in downstream cell and molecular biology use.
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3
0.1 µm Sterile-filtration I
Second-pass 0.1 µm filtration provides redundant sterile filtration ahead of final polish.
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4
0.04 µm Sterile-filtration II — Final Polish
Final 0.04 µm polish is performed in a sterile environment, consistent with practices intended to prevent mycoplasma contamination.
Performance vs. conventional buffer
Sequential 0.1 µm and 0.04 µm filtration, applied twice each, removes finer particulates than a single conventional 0.22 µm pass, supporting cleaner runs in microfluidic electrophoresis channels and lower background in sensitive detection assays.
© Diagnocine® — DCP-TGSB1X
Where Tris-Glycine SDS Buffer [1X] is used
Tris-Glycine SDS Buffer [1X] is commonly used as a running buffer for SDS-PAGE. Sodium dodecyl sulfate (SDS) is an anionic detergent that denatures proteins, unfolding them and applying a uniform negative charge, disrupting native structure and reducing proteins to their primary polypeptide chain so they can be separated by molecular weight during electrophoresis. Glycine helps concentrate protein samples and move them through the stacking gel layer, while Tris and glycine provide the ions that carry current through the gel and serve as both anode and cathode buffer. Its alkaline pH supports effective protein separation.
Automated Bioreactors & Robotics
For automated liquid-handling platforms and robotics-driven electrophoresis or bioreactor workflows, an optional 0.01 µm (10 nm) ultra-filtered variant of this buffer is available on request.
- Total Particulate Exclusion — minimizes fine particulate load in automated fluid paths
- Valve & Sensor Protection — reduces particulate exposure to precision valves and inline sensors
- Extended Perfusion Stability — supports longer unattended run times in automated systems
Inquiry Required: The 0.01 µm ultra-filtered grade is available by request — contact support@diagnocine.com.
SDS-PAGE Running Buffer
Used directly as the running buffer for SDS-PAGE, providing the ionic environment needed for consistent current flow and protein migration.
Blocking Buffer Base
Serves as the base buffer to block membranes with nonfat dry milk for western blotting workflows.
Protein Denaturation & Charge Loading
SDS in the buffer denatures proteins and applies a uniform negative charge, enabling separation strictly by molecular weight and reducing gel deformation during the run.
Nucleic Acid-Safe Reagent Handling
DNase- and RNase-tested formulation supports use in workflows adjacent to plasmid DNA and RNA handling without nuclease carryover concerns.
Micro Physiological System (MPS) & Chip-Based Electrophoresis
Ultra-filtered purity supports use in microfluidic and chip-based electrophoresis formats where channel fouling from particulates must be minimized.
Low-Background Buffer for Imaging & Detection
Sterile, ultra-filtered formulation helps keep background low in chemiluminescent and fluorescent western blot detection.
Detailed specifications
Every specification below reflects the tested and declared properties of Tris-Glycine SDS Buffer [1X], catalog number DCP-TGSB1X.
| Parameter | Specification |
|---|---|
| Formulation | Tris-Glycine-SDS running buffer, 1X |
| Appearance | Clear, colorless liquid |
| pH USP <791> | 8.3 (± 0.05) |
| Tris base concentration | 25 mM |
| Glycine concentration | 192 mM |
| SDS concentration | 0.1% |
| Parameter | Specification |
|---|---|
| Sterility | Filtered 0.1 µm membrane twice in a sterile environment |
| DNase activity | None detected after 18 hr incubation with plasmid DNA at room temperature |
| RNase activity | None detected after 18 hr incubation with ribosomal RNA at room temperature |
| Water quality | Ultrapure Type 1 water (18.2 MΩ·cm) |
| Fill environment | ISO Class 5 (Class 100) |
| Parameter | Specification |
|---|---|
| Storage temperature | Room temperature |
| Shelf life | 2 years |
| Parameter | Specification |
|---|---|
| Manufacturing QMS ISO 13485 | Manufactured under ISO 13485-certified facilities |
| Regulatory alignment | CE-approved facilities (Suppliers of DiagnoCine Precision) |
| Production & QA site | Final packaging, QA, and testing at the DiagnoCine R&D and Quality Testing Center; customization and assembly at DiagnoCine Precision, Totowa, New Jersey, USA |
| Intended use | Research Use Only (RUO) |
Full composition
Tris-Glycine SDS Buffer [1X] is a defined three-component buffering system prepared to the same specification on every lot.
| Component | CAS Number | Concentration |
|---|---|---|
| Tris base | 77-86-1 | 25 mM |
| Glycine | 56-40-6 | 192 mM |
| SDS | 151-21-3 | 0.1% |
Manufacturing & compliance
Tris-Glycine SDS Buffer [1X] is manufactured, quality-tested, and released under a documented quality system.
ISO 13485:2016 QMS
Manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision).
Ultrapure Type 1 Water
Formulated using ultrapure Type 1 water (18.2 MΩ·cm) as the base for every batch.
ISO Class 5 Fill & Finish
Final packaging performed under controlled aseptic fill conditions to maintain sterility.
Micro-Batch Precision
All specific customization requests and assembly are accomplished at DiagnoCine Precision in Totowa, New Jersey, USA.
DNase Activity
None detected after 18 hr incubation of plasmid DNA with this product at room temperature.
RNase Activity
None detected after 18 hr incubation of ribosomal RNA with this product at room temperature.
Sterility
Filtered with 0.1 µm membrane filtration twice and 0.04 µm membrane filtration twice in a sterile environment.
Documentation / CoA
Certificate of Analysis available for this lot upon request.
How DCP-TGSB1X compares
A side-by-side look at how FluxMPS™ Tris-Glycine SDS Buffer [1X] compares with conventional running buffer.
| Parameter | DCP-TGSB1X (FluxMPS™) | Conventional 0.22 µm-filtered buffer | Standard alternative (0.22 µm-filtered buffer) |
|---|---|---|---|
| Defined Tris/Glycine/SDS molarity (25 mM/192 mM/0.1%) | check_circle | cancel | cancel |
| Final filtration pore size | 0.04 µm | 0.22 µm | 0.22 µm |
| Number of filtration stages | 4 | 1 | 1 |
| DNase / RNase tested | check_circle | cancel | cancel |
| Sterile-filtered in controlled environment | check_circle | cancel | cancel |
| Manufacturing QMS (ISO 13485) | check_circle | cancel | cancel |
| Water quality | Ultrapure Type 1 (18.2 MΩ·cm) | Standard purified water | Standard purified water |
| Microfluidic / electrophoresis-chip compatibility | check_circle | cancel | cancel |
| Custom formulation available | check_circle | cancel | cancel |
Frequently asked questions
Common questions about FluxMPS™ Tris-Glycine SDS Buffer [1X], catalog number DCP-TGSB1X.
Supporting literature
Foundational and applied literature relevant to Tris-Glycine SDS buffer chemistry and its use in electrophoresis, western blotting, and microfluidic protein separation.
- Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970. doi:10.1038/227680a0
- Shapiro AL, Vinuela E, Maizel JV. Molecular weight estimation of polypeptide chains by electrophoresis in SDS-polyacrylamide gels. Biochem Biophys Res Commun. 1967. doi:10.1016/0006-291X(67)90391-9
- Reynolds JA, Tanford C. Binding of dodecyl sulfate to proteins at high binding ratios. Proc Natl Acad Sci USA. 1970. doi:10.1073/pnas.66.3.1002
- Towbin H, Staehelin T, Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets. Proc Natl Acad Sci USA. 1979. doi:10.1073/pnas.76.9.4350
- Ornstein L. Disc electrophoresis. I. Background and theory. Ann N Y Acad Sci. 1964. doi:10.1111/j.1749-6632.1964.tb14126.x
- Good NE, et al. Hydrogen ion buffers for biological research. Biochemistry. 1966. doi:10.1021/bi00866a011
- Mahmood T, Yang PC. Western blot: technique, theory, and trouble shooting. N Am J Med Sci. 2012. doi:10.4103/1947-2714.100998
- Kurien BT, Scofield RH. Western blotting. Methods. 2006. doi:10.1016/j.ymeth.2005.07.018
- Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014. doi:10.1038/nbt.2989