Buffer Set for CATAROSEV™, (100 mL x 3)
FluxMPS™ Buffer Set for CATAROSEV™ (100 mL x 3)
An MPS-grade, room-temperature-stable wash and elution buffer set built for the CATAROSEV™ membrane-based exosome recovery workflow. The three-solution set (adsorption, washing, elution) is Tris (trometamol)-buffered and formulated with a controlled sodium chloride profile to support selective, high-yield capture and release of extracellular vesicles from cell culture supernatant.
- Purpose-built three-solution buffer set for the CATAROSEV™ adsorption, washing, and elution workflow
- Room-temperature-stable format, packaged as 100 mL x 3
- Trometamol (Tris)-buffered chemistry, CAS 77-86-1
- Sodium chloride content tuned per step (0–1.8%) to drive selective elution
- Ultrapure water base, CAS 7732-18-5
- Manufactured using single-stage 0.1 µm membrane filtration
- Supports exosome recovery in approximately 30 minutes with the CATAROSEV™ membrane*
- Custom pH, molarity, and salt content available on request
- Packaging
- 100 mL x 3
- Storage Temperature
- Room Temperature
- Primary Buffer
- Trometamol (Tris), CAS 77-86-1
- Trometamol Content
- 0.1–0.1% (w/v)
- Sodium Chloride Content
- 0–1.8% (w/v)
- Water Content
- 98.1–99.9% (w/v)
- Workflow Steps
- Adsorption, Washing, Elution
- Recovery Time*
- Approximately 30 minutes
- Compatible System
- CATAROSEV™ exosome recovery membrane kit
- Format
- 3 x 100 mL bottles
Engineered for the CATAROSEV™ recovery workflow
This buffer set supports the CATAROSEV™ exosome recovery technology, originally developed by Toyobo Co., Ltd. and offered under the FluxMPS™ line by Diagnocine, so the adsorption, wash, and elution chemistry works cleanly with the membrane's charged pores instead of introducing particulate, pH, or ionic drift into the workflow.
Membrane-Compatible Purity
Formulated for compatibility with fine-pore separation membranes such as the CATAROSEV™ positively charged capture membrane (approximately 200 nanometers in diameter), supporting clean adsorption, wash, and elution steps.
Consistent, Tris-Buffered Chemistry
Buffered with trometamol (Tris, CAS 77-86-1) to help maintain consistent conditions across the adsorption, washing, and elution steps of the recovery workflow.
Ultrapure-Grade Water Base
Manufactured with Ultrapure Type 1 water (18.2 MΩ·cm), providing a low-background aqueous base for the buffer set.
Assay- and Analysis-Ready
Compatible with downstream characterization methods used to confirm exosome recovery, including nanoparticle tracking analysis (NanoSight) and Western blotting for TSG101, CD63, and CD9.
Defined, Traceable Composition
Every component — trometamol, sodium chloride, and water — is listed with CAS number and chemical substance gazette reference for regulatory traceability.
Customization on Demand
pH, molarity, salt concentration, and additive content of the adsorption, wash, and elution solutions are available on request — contact support@diagnocine.com.
Single-stage 0.1 µm filtration
Each of the three solutions in this buffer set is manufactured using a single-pass 0.1 µm membrane filtration step that removes large particulates and aggregates prior to fill, supporting a clean, room-temperature-stable format for the CATAROSEV™ workflow.
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0.1 µm Pre-filtration / Sterile-filtration
Single-pass 0.1 µm membrane filtration removes large particulates and aggregates from each of the three solutions prior to fill.
Performance vs. conventional buffer
Single-pass 0.1 µm filtration provides a clean, particulate-reduced format appropriate for room-temperature-stable wash and elution buffers used in membrane-based exosome recovery workflows.
© Diagnocine® — TYB-CTS-011
Where this buffer set fits
This wash/elution buffer set is designed for the CATAROSEV™ exosome recovery workflow, and its Tris/sodium-chloride chemistry is broadly relevant to membrane-based extracellular vesicle isolation, sample wash, and elution steps used across microfluidic and lab-scale workflows.
The CATAROSEV™ membrane-based recovery system this buffer set supports uses a positively charged separation membrane with pores approximately 200 nanometers in diameter to selectively capture exosomes and other fine particles 50 to 150 nanometers in diameter through electrostatic adsorption, followed by a wash step and a salt-based elution step, completing recovery from 5 mL of culture supernatant in approximately 30 minutes.[1] In internal comparison studies (not independently guaranteed), this technique yielded approximately 50% more exosomes than the ultracentrifugation method and more than 10 times more than a magnetic-bead-based method, with recovery confirmed by nanoparticle tracking analysis (NanoSight NS300) and Western blotting for TSG101, CD63, and CD9.
Automated Bioreactors & Robotics
For automated, closed-loop bioreactor and robotic liquid-handling platforms, an optional 0.01 µm (10 nm) ultra-filtered variant of this buffer set is available on request to further reduce particulate load in long-duration, unattended runs.
- Total Particulate Exclusion
- Valve & Sensor Protection
- Extended Perfusion Stability
Inquiry Required: The 0.01 µm (10 nm) ultra-filtered grade is produced to order; contact support@diagnocine.com to request this configuration.
Micro Physiological System (MPS) & Chip
Wash and rinse buffer chemistry suitable for sample handling steps within microfluidic and chip-based workflows.
Wash, Dilution & Reconstitution
Formulated as a three-part adsorption, wash, and elution solution set for membrane-based exosome capture workflows such as the CATAROSEV™ system.
iPSC-Derived Model Handling
Wash and rinse buffers of this type support sample handling around iPSC-derived and other primary cell models feeding into downstream extracellular vesicle recovery.
Endothelial & Primary Cell Perfusion
Room-temperature-stable wash chemistry appropriate for sample handling steps around endothelial and primary cell culture supernatants.
ELISA, Blotting & Blocking
Compatible with downstream immunoassay confirmation of recovered exosomes, including Western blotting for TSG101, CD63, and CD9 markers.
Microscopy & Optical Sensing
Supports nanoparticle tracking analysis (e.g., NanoSight NS300) and other optical characterization methods used to confirm particle size and concentration of recovered exosomes.
Specifications
Values below reflect only what is documented for this buffer set; parameters not specified for this SKU are omitted rather than estimated.
| Parameter | Specification |
|---|---|
| Formulation / Composition | Trometamol (Tris), sodium chloride, water |
| Trometamol Content | 0.1–0.1% (w/v) |
| Sodium Chloride Content | 0–1.8% (w/v) |
| Water Content | 98.1–99.9% (w/v) |
| Parameter | Specification |
|---|---|
| Filtration USP | Single-stage 0.1 µm membrane filtration |
| Water Quality | Ultrapure Type 1 water, 18.2 MΩ·cm |
| Manufacturing Standard ISO | ISO 13485:2016 |
| Fill Environment | ISO Class 5 (Class 100) |
| Parameter | Specification |
|---|---|
| Storage Temperature | Room Temperature |
| Packaging | 100 mL x 3 |
| Parameter | Specification |
|---|---|
| Raw Material Identity | Trometamol (CAS 77-86-1), sodium chloride (CAS 7647-14-5), water (CAS 7732-18-5) |
| Regulatory Traceability | Japan CSCL / ISHL gazette reference numbers on file per component |
| Manufacturing QMS | ISO 13485:2016 |
| Intended Use | Research Use Only (RUO) |
Full composition
The buffer set contains three room-temperature-stable solutions (adsorption, wash, elution); concentration ranges below reflect the variation across these three solutions, as documented in the product's composition and ingredient disclosure.
| Component | CAS Number | Concentration |
|---|---|---|
| Trometamol (Tris) | 77-86-1 | 0.1–0.1% |
| Sodium chloride | 7647-14-5 | 0–1.8% |
| Water | 7732-18-5 | 98.1–99.9% |
Manufacturing & compliance
Manufactured under a documented quality system with lot traceability from raw material through fill.
ISO 13485:2016 QMS
Manufactured under an ISO 13485:2016-aligned quality management system.
Ultrapure Type 1 Water
Formulated with Ultrapure Type 1 water, 18.2 MΩ·cm.
ISO Class 5 Fill & Finish
Filled in an ISO Class 5 (Class 100) environment.
Micro-Batch Precision
Component-level CAS and regulatory gazette traceability across the three-solution set.
Endotoxin Testing
Endotoxin testing, when performed, follows USP <85> Bacterial Endotoxins Test methodology; lot-specific results are available via Certificate of Analysis.
Particulate Testing
Particulate testing, when performed, follows USP <788> Method 2; lot-specific results are available via Certificate of Analysis.
Osmolality Testing
Osmolality testing, when performed, follows USP <785>; contact support@diagnocine.com for lot-specific data.
Documentation / CoA
A Certificate of Analysis is available for this lot on request.
How TYB-CTS-011 compares
A general comparison against conventional buffer manufacturing practices.
| Parameter | TYB-CTS-011 (FluxMPS™) | Conventional version (0.22 µm filtered buffer) | Standard alternative (0.22 µm filtered buffer) |
|---|---|---|---|
| ISO 13485:2016 manufacturing | check_circle | cancel | cancel |
| Ultrapure Type 1 water (18.2 MΩ·cm) | check_circle | cancel | cancel |
| Regulatory-traceable raw materials (CAS + gazette reference) | check_circle | cancel | cancel |
| Single-stage 0.1 µm membrane filtration | check_circle | cancel | cancel |
| Custom molarity / salt formulation available | check_circle | cancel | cancel |
| Certificate of Analysis on request | check_circle | cancel | cancel |
Frequently asked questions
Common questions about the Buffer Set for CATAROSEV™ (TYB-CTS-011).
Supporting literature
Curated literature on extracellular vesicle biology, isolation methods, and characterization techniques relevant to this buffer set's use.
- Thery C, Witwer KW, Aikawa E, et al. Minimal information for studies of extracellular vesicles 2018 (MISEV2018). doi:10.1080/20013078.2018.1535750
- Livshits MA, Khomyakova E, Evtushenko EG, et al. Isolation of exosomes by differential centrifugation: theoretical analysis of a commonly used protocol. doi:10.1038/srep17319
- Bordas M, Genard G, Ohl S, et al. Comparison of isolation methods for extracellular vesicles from cell culture supernatant. doi:10.1002/jev2.12008
- Dragovic RA, Gardiner C, Brooks AS, et al. Sizing and phenotyping of cellular vesicles using nanoparticle tracking analysis. doi:10.1016/j.nano.2011.04.003
- Thery C, Amigorena S, Raposo G, Clayton A. Isolation and characterization of exosomes from cell culture supernatants and biological fluids. doi:10.1002/0471143030.cb0322s30
- Kowal J, Arras G, Colombo M, et al. Proteomic comparison defines novel markers to characterize heterogeneous populations of extracellular vesicle subtypes. doi:10.1073/pnas.1521230113
- Konoshenko MY, Lekchnov EA, Vlassov AV, Laktionov PP. Isolation of extracellular vesicles: general methodologies and latest trends. doi:10.1155/2018/8545347
- Good DM, Zurbig P, Argiles A, et al. Naturally occurring human urinary peptides for use in diagnosis of chronic kidney disease. doi:10.1074/mcp.M110.001917
