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FluxMPS™ Ham's F-10 Nutrient Mixture with 25mM HEPES: 1X Liquid

Product#: DCP-H10H1X
$57.20
DCP-H10H1X
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warning For Research Use Only (RUO). Not intended for clinical, diagnostic, or therapeutic use in humans.
verified ISO 13485 Certified Manufacturing

FluxMPS™ Ham's F-10 Nutrient Mixture with 25mM HEPES: 1X Liquid

Contains L-Glutamine Contains Sodium Bicarbonate Contains Phenol Red Contains HEPES Contains Calcium Contains Magnesium Contains Glucose Contains Sodium Pyruvate

FluxMPS™ DCP-H10H1X is a Microfluidics Suitable, quadruple-stage ultra-filtered Ham's F-10 + 25mM HEPES formulation engineered for CHO cells and related cell models on organ-on-a-chip (OoC) and microphysiological system (MPS) platforms. A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration. HEPES (25 mM, pKa 7.3 at 37°C) provides robust pH buffering. Formulation: [+] Glucose (1100 mg/L), [+] L-Glutamine, [+] Sodium Pyruvate, [+] Sodium Bicarbonate, [+] 25mM HEPES.

  • Glucose: 1100 mg/L (1.1 g/L) — lower than Ham's F-12 (1.8 g/L)
  • 25 mM HEPES (pKa 7.3 at 37°C) — supplemental pH buffering independent of CO₂ tension
  • Zinc, hypoxanthine, thymidine, lipoic acid — trace nutrients supporting CHO and primary fibroblast clonal growth
  • 0.04 µm final filtration stage; endotoxin release specification < 0.05 EU/mL (USP <85>)
  • Quadruple-stage filtration: 0.1 µm Prefiltration I → 0.04 µm Final filtration I → 0.1 µm Prefiltration II → 0.04 µm Final filtration II — Polish
  • Ultrapure Type 1 water (18.2 MΩ·cm), ISO 13485:2016 QMS, ISO Class 5 fill
CAT. NO.
DCP-H10H1X | Cell Culture Media UNSPSC: 41116155 | Commodity: Molecular biology and cell culture growth media | (UNv260801)
Ham's F-10 + 25mM HEPES — 1X Liquid
  • Media familyHam's F-10 + 25mM HEPES
  • Glucose1100 mg/L (1.1 g/L)
  • HEPES25 mM, pKa 7.3 at 37°C
  • pH (USP <791>)7.4
  • Osmolality (USP <785>)Contact for specification
  • Endotoxin (USP <85>)< 0.05 EU/mL
  • Filtration0.1 µm ×2 + 0.04 µm ×2
  • Storage2–8°C, away from light
  • Shelf Life12 months from date of manufacture, unopened
  • ShippingCold pack
ISO 13485:2016 USP <85> <785> <788> RUO
Why FluxMPS™

Engineered where standard media fails

Conventional 0.22 µm–filtered Ham's passes mycoplasma-sized particles, subvisible particulates, and endotoxin fragments that can accumulate in microfluidic channels. FluxMPS™ is built to reduce these failure modes.

filter_alt

Microchannel-safe purity

0.04 µm final filtration; USP <788> Method 1 particulate compliance. Supports low-clog perfusion across a wide range of chip geometries.

science

Cell-type–optimized formulation

Ham's F-10 contains zinc, hypoxanthine, thymidine, and lipoic acid supporting CHO cell and primary fibroblast clonal growth.

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Ultrapure-grade water

18.2 MΩ·cm Type 1 water reduces trace-metal and organic-carbon background in the finished medium.

shield

Below TLR4 endotoxin threshold

< 0.05 EU/mL release specification — supports assays sensitive to LPS-driven TLR4 activation in primary cells.

water_drop

HEPES pH stability

HEPES (25 mM) provides supplemental pH buffering during open-air handling, flow cytometry preparation, and atmospheric incubation.

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Customization on demand

pH, nutrient concentrations, HEPES, and component modifications available. Contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

Four serial filtration stages — two dedicated prefilter + final-filter pairs — reaching a final 0.04 µm polish under ISO Class 5 aseptic conditions.

  1. 1

    0.1 µm Prefiltration I

    Removes large aggregates, cell debris, and protein aggregates; protects the first 0.04 µm cartridge.

  2. 2

    0.04 µm Final filtration I

    Retains sub-micron particulates and microaggregates that pass a standard 0.22 µm filter.

  3. 3

    0.1 µm Prefiltration II

    Second dedicated prefilter, protecting the second 0.04 µm cartridge.

  4. 4

    0.04 µm Final filtration II — Polish

    Ultimate polishing filter; aseptic fill & finish in a validated ISO Class 5 environment.

Performance vs. conventional media

5×
Cleaner than 0.22 µm media by particulate count
0.04
µm Final pore size — sub-mycoplasma polishing
Sterility & Mycoplasma: No growth after 14-day incubation (USP <71>); 0.1 µm mycoplasma-retentive filtration (0.2–0.3 µm organisms) applied at every production stage (not tested per lot).
Grade: This product is Microfluidics Suitable, filtered to a 0.04 µm final cut-off. It is not an MPS Grade product — that designation is reserved for the 0.01 µm ultra nano-filtered line, which adds 0.02 µm and 0.01 µm stages after the 0.04 µm polish. For applications requiring the 0.01 µm cut-off, contact support@diagnocine.com.
FluxMPS DCP-H10H1X Ham's F-10 Nutrient Mixture with 25mM HEPES quadruple-stage 0.1 micron times two plus 0.04 micron times two filtration system for organ-on-a-chip and microfluidic cell culture applications, Diagnocine
Figure 1. FluxMPS™ quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2) applied to DCP-H10H1X Ham's F-10 + 25mM HEPES.
© Diagnocine® — DCP-H10H1X
Applications

Specialized cell culture applications

FluxMPS™ DCP-H10H1X — Ham's F-10 + 25mM HEPES — delivers 0.04 µm filtered purity for CHO cells and related OoC applications.

Automated Bioreactors & Robotics

Next-Generation System Uptime

An optional 0.01 µm (10 nm) MPS Grade ultra nano-filtered variant of this formulation is available on request for automated perfusion systems.

  • Total Particulate Exclusion: 10 nm filtration removes nanoparticulate aggregates
  • Valve & Sensor Protection: Reduces micro-fouling risk in automated perfusion systems
  • Extended Perfusion Stability: Consistent nutrient delivery over long-duration culture

Inquiry Required: Contact support@diagnocine.com for the 0.01 µm MPS Grade variant.

Bioproduction

CHO & Mammalian Cell Culture

Optimized for CHO, hybridoma, myeloma, and primary fibroblast culture in serum-free or low-serum conditions.

CHOHybridomaMyeloma
Microfluidics

Organ-on-a-Chip & MPS

0.04 µm filtered media reduces the risk of microchannel clogging in complex multi-organ chip architectures.

OoCToCMPS
Cancer Biology

Cancer Cell Lines

Supports fibroblast co-culture tumor models and primary cancer cell culture in a defined nutrient environment.

Tumor modelsFibroblastsCo-culture
Stem Cell Biology

Primary & Stem Cell Support

Rich nutrient profile with trace elements supports primary cell viability and stem cell maintenance in low-serum conditions.

Primary cellsMSCsiPSC co-culture
Metabolomics

Metabolic Flux Analysis

Defined nutrient base for ¹³C isotope tracing in specialized cell culture models. Not compatible with Agilent Seahorse XF assays, which require bicarbonate-free, phenol red-free medium.

¹³C tracingMetabolomics
Live-Cell Imaging

Microscopy & Optical Sensing

Ultra-low particulate baseline supports confocal microscopy and biosensor applications; phenol red-free formulations of this line are available on request for autofluorescence-sensitive imaging.

ConfocalTEERBiosensors
Technical Specifications

Analytical release specifications

Every lot released against the full specification matrix. CoA: support@diagnocine.com.

Physical & Chemical Parameters
Parameter Specification
Formulation [+] L-Glutamine, [+] Sodium Bicarbonate, [+] Phenol Red, [+] HEPES (25 mM), [+] Calcium, [+] Magnesium, [+] Glucose (1100 mg/L), [+] Sodium Pyruvate
Appearance Red-colored, clear solution
Glucose 1100 mg/L (1.1 g/L)
HEPES 25 mM (pKa 7.3 at 37°C)
pH USP <791> 7.4
Osmolality USP <785> Contact for specification
Available pack sizes 500 mL, 1000 mL
Total ingredients 47
Sterility, Purity & Safety
Parameter Specification
Endotoxin USP <85> BET < 0.05 EU/mL (batch release specification — see Manufacturing & Compliance)
Sterility USP <71> No growth / 14 days
Mycoplasma 0.1 µm mycoplasma-retentive filtration (not tested per lot)
Particulate ≥10 µm USP <788> Method 1 NMT 25/mL
Particulate ≥25 µm USP <788> Method 1 NMT 3/mL
Water purity Type 1, 18.2 MΩ·cm
Manufacturing std. ISO 13485:2016
Fill environment ISO Class 5 (Class 100)
Storage, Handling & Logistics
Parameter Specification
Storage temperature 2–8°C, away from light
Freeze-thaw Do not freeze
Shelf life 12 months from date of manufacture, unopened
Shipping condition Cold pack
CO₂ requirement 5% CO₂ recommended; the 25 mM HEPES component provides supplemental pH buffering independent of CO₂ tension
Raw Materials & Regulatory
Parameter Specification
Raw material grade Reagent / cell culture grade
Traceability Full lot traceability per ISO 13485
Manufacturing QMS ISO ISO 13485:2016 certified
UNSPSC 41116155 — Molecular biology and cell culture growth media (UNv260801)
Regulatory alignment 21 CFR Part 820 (QMSR) aligned
Production method Micro-batch, per-lot QC release
Intended use Research Use Only (RUO)
Formulation

Full composition (mg/L)

Ham's F-10 + 25mM HEPES: 47 ingredients verified per lot with CAS numbers for full raw-material traceability. Ham's F-10 contains zinc, hypoxanthine, thymidine, and lipoic acid supporting CHO cell and primary fibroblast clonal growth.

Component CAS Number mg/L
INORGANIC SALTS
Calcium chloride dihydrate 10035-04-8 44.100
Copper sulfate pentahydrate 7758-99-8 0.0025
Ferrous sulfate heptahydrate 7782-63-0 0.834
Magnesium chloride anhydrous 7786-30-3 74.640
Potassium chloride 7447-40-7 285.000
Potassium phosphate monobasic 7778-77-0 83.000
Sodium bicarbonate 144-55-8 1200.000
Sodium chloride 7647-14-5 7400.000
Sodium phosphate dibasic anhydrous 7558-79-4 153.700
Zinc sulfate heptahydrate 7446-20-0 0.0288
Component CAS Number mg/L
AMINO ACIDS
Glycine 56-40-6 7.500
L-Alanine 56-41-7 8.910
L-Arginine hydrochloride 1119-34-2 211.000
L-Asparagine monohydrate 5794-13-8 15.010
L-Aspartic acid 56-84-8 13.300
L-Cystine dihydrochloride 30925-07-6 35.120
L-Glutamic acid 56-86-0 14.700
L-Glutamine 56-85-9 146.000
L-Histidine hydrochloride monohydrate 5934-29-2 21.000
L-Isoleucine 73-32-5 2.600
L-Leucine 61-90-5 13.100
L-Lysine hydrochloride 657-27-2 29.300
L-Methionine 63-68-3 4.480
L-Phenylalanine 63-91-2 4.960
L-Proline 147-85-3 11.500
L-Serine 56-45-1 10.500
L-Threonine 72-19-5 3.570
L-Tryptophan 73-22-3 0.600
L-Tyrosine disodium salt dihydrate 69847-45-6 2.610
L-Valine 72-18-4 3.500
Component CAS Number mg/L
VITAMINS
Biotin 58-85-5 0.024
Choline chloride 67-48-1 0.698
D-Ca-Pantothenate 137-08-6 0.715
Folic acid 59-30-3 1.320
Nicotinamide 98-92-0 0.615
Pyridoxal hydrochloride 65-22-5 0.206
Riboflavin 83-88-5 0.376
Thiamine hydrochloride 67-03-8 1.000
Vitamin B12 68-19-9 1.360
OTHERS
i-Inositol 87-89-8 0.541
D-Glucose 50-99-7 1100.000
HEPES buffer 7365-45-9 5958.000
Hypoxanthine Sodium Salt 5765-44-6 4.080
Lipoic acid 1077-28-7 0.210
Phenol red Sodium Salt 34487-61-1 1.300
Sodium pyruvate 113-24-6 110.000
Thymidine 50-89-5 0.730
Custom formulation: Contact support@diagnocine.com for DCP-H10H1X modifications.
Quality Assurance

Manufacturing & compliance

Every FluxMPS™ product is manufactured and released under a multi-layer quality system.

verified

ISO 13485:2016 Quality Management

Manufactured under an ISO 13485:2016 quality management system. Final QA at Diagnocine R&D Center, Totowa, NJ, USA.

water_drop

Ultrapure Type 1 Water

18.2 MΩ·cm — reduces trace-metal and organic-carbon background.

biotech

ISO Class 5 Fill & Finish

Aseptic fill in validated ISO Class 5 (Class 100) laminar-flow workstations.

assignment

Micro-Batch Precision

Small-batch, per-lot tested — no blending; Certificate of Analysis available for every lot.

Endotoxin — USP <85> BET

LAL assay; batch release specification < 0.05 EU/mL.

Particulate — USP <788> Method 1

Light obscuration; NMT 25/mL (≥10 µm), NMT 3/mL (≥25 µm).

Osmolality — USP <785>

Target: Contact for specification.

Documentation & CoA

Full CoA with raw-material traceability available on request.

Batch-level quality control. Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested before release and must meet the release specification:
  • Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
  • pH, osmolality, conductivity, appearance and clarity
  • Sterility
A Certificate of Analysis is available on request at support@diagnocine.com.
Product Comparison

How DCP-H10H1X compares

FluxMPS™ DCP-H10H1X vs. conventional 0.22 µm–filtered Ham's formulations.

Parameter DCP-H10H1X (FluxMPS™) Conventional Ham's F-10 + 25
(0.22 µm filtered)		 Standard DMEM/RPMI
(0.22 µm filtered)
Grade Microfluidics Suitable (0.04 µm final) Not specified Not specified
Ham's F-10 + 25mM HEPES formulation — zinc, hypoxanthine, thymidine, lipoic acid for CHO and primary fibroblast OoC check_circle Yes cancel No cancel No
HEPES buffering (25 mM) check_circle Yes cancel Usually no cancel No
Final filtration pore size 0.04 µm 0.22 µm 0.22 µm
Number of filtration stages 4 (Quadruple) 1 1
Mycoplasma-retentive filtration check_circle Yes (0.1 µm) cancel No cancel No
Endotoxin (release specification) < 0.05 EU/mL Corning classical liquid media — < 0.25 EU/mL
Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL
Gibco classical DMEM — Not specified (recorded per lot)
USP <788> Method 1 particulate tested check_circle Yes cancel No cancel No
Water quality Type 1, 18.2 MΩ·cm Purified water Purified water
Manufacturing QMS ISO 13485:2016 ISO 9001 or none ISO 9001 or none
Microfluidic channel compatible check_circle Yes — Microfluidics Suitable cancel Risk of clogging cancel Risk of clogging
Custom formulation available check_circle Yes cancel No cancel No

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

FAQ

Frequently asked questions

Common questions about FluxMPS™ DCP-H10H1X — Ham's F-10 + 25mM HEPES.

Yes. DCP-H10H1X is Microfluidics Suitable, processed through a quadruple-stage filtration system reaching a 0.04 µm final pore size. Ham's F-10 + 25mM HEPES with 0.04 µm filtration is suited to specialized OoC and MPS models that require this specific medium formulation.
 
Ham's F-10 contains zinc, hypoxanthine, thymidine, and lipoic acid — trace nutrients absent from F-12 — supporting clonal growth of CHO cells and primary fibroblasts in serum-free conditions. It uses lower glucose (1.1 g/L) than F-12 (1.8 g/L). The 25 mM HEPES provides supplemental, CO₂-independent pH buffering, useful for CHO culture in automated bioreactors where CO₂ fluctuation between batches can cause pH drift. FluxMPS™ 0.04 µm filtration reduces particulate load on these trace nutrients.
5% CO₂ is recommended. This formulation carries both sodium bicarbonate and 25 mM HEPES; the HEPES component provides supplemental pH buffering independent of CO₂ tension, but the bicarbonate system is calibrated for a 5% CO₂ environment.
Yes. Add FBS (5–10%), serum-free supplements, growth factors, or antibiotics as required. When filtering serum or protein-containing supplements before addition, use a 0.2 µm low-protein-binding PES or PVDF filter — a 0.04 µm membrane will strip serum of the components that make it effective and is not recommended for supplement filtration. Contact support@diagnocine.com for custom co-formulation.
DCP-H10H1X carries a batch release specification of < 0.05 EU/mL by LAL assay (USP <85>, assay sensitivity 0.005 EU/mL). Endotoxin is controlled per manufacturing batch: every batch is tested before release and must meet this specification. Low endotoxin is relevant to primary cell culture, where endotoxin can activate TLR4 signaling.
Yes. Full CoA per lot covers: appearance, pH (USP <791>), osmolality (USP <785>), sterility (USP <71>), endotoxin (USP <85>), mycoplasma-retentive filtration status, particulate count (USP <788> Method 1), lot number, expiry, and raw-material traceability. Request at support@diagnocine.com.
Scientific References

Supporting literature

Key publications supporting Ham's F-10 + 25mM HEPES in specialized cell culture and OoC applications.

  1. Ham RG. Clonal growth of mammalian cells in a chemically defined, synthetic medium. Proc Natl Acad Sci USA. 1965;53:288–293. doi:10.1073/pnas.53.2.288
  2. Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
  3. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
  4. Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x
  5. Kao FT, Puck TT. Genetics of somatic mammalian cells: quantitative survival of Chinese hamster cells with defined mutations. Genetics. 1969;62:369–376. doi:10.1093/genetics/62.2.369
  6. Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588–3598. doi:10.1039/c3lc50217a
  7. Good NE, et al. Hydrogen ion buffers for biological research. Biochemistry. 1966;5:467–477. doi:10.1021/bi00866a011
  8. Sung JH, et al. Microfabricated mammalian organ systems. Lab Chip. 2013;13:1201–1212. doi:10.1039/c3lc41017j

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