Nuclease-Free Water (not DEPC-Treated)
FluxMPS™ Nuclease-Free Water (not DEPC-Treated)
FluxMPS™ Nuclease-Free Water is an MPS-grade, ultrapure molecular biology reagent purified through a quadruple-stage filtration system and manufactured without DEPC treatment. Sterile, autoclaved, and rigorously tested for the absence of RNase and DNase activity, it is engineered for sensitive PCR, RNA/DNA extraction, and microfluidic reagent-preparation workflows where nuclease contamination and DEPC residues cannot be tolerated.
- Quadruple-stage filtration: 0.1 µm membrane filtration performed twice and 0.04 µm membrane filtration performed twice
- Not DEPC-treated — eliminates the risk of DEPC residue interfering with sensitive enzymatic reactions
- pH 7.4, colorless, clear solution; sterile and autoclaved
- Rigorously tested to confirm the absence of detectable RNase and DNase activity
- Manufactured under ISO 13485-certified, CE-approved facilities
- Ready-to-use, sterile packaging minimizes contamination risk
- Available in 100 mL, 500 mL, and 10 x 50 mL multi-pack formats
- Custom pH and additive formulations available on request
- Product TypeNuclease-Free Water
- pH7.4
- AppearanceColorless, clear solution
- SterilitySterile (autoclaved)
- Filtration0.1 µm x2 + 0.04 µm x2
- DEPC TreatmentNot DEPC-treated
- Nuclease ActivityNot detected (RNase/DNase-free)
- StorageRoom Temperature
- Shelf Life36 months
- ManufacturingISO 13485-certified / CE-approved
Engineered where standard nuclease-free water falls short
Conventional 0.22 µm-filtered molecular biology water can carry subvisible particulates, residual bioburden, and—when DEPC-treated—chemical residues that interfere with sensitive enzymatic reactions. FluxMPS™ Nuclease-Free Water is built to remove those failure modes at the source.
Microchannel-Safe Purity
Final 0.04 µm membrane stage removes fine particulates that can accumulate in narrow microfluidic channels and valves.
Precise, Stable pH
Formulated to a defined pH of 7.4, giving reproducible chemistry for downstream enzymatic and molecular biology reactions.
Ultrapure-Grade Water
Produced from ultrapure Type 1 water (18.2 MΩ·cm), autoclaved and sterile-filtered for molecular biology-grade quality.
Low Background for Sensitive Assays
Confirmed absence of detectable RNase/DNase activity supports clean, low-background PCR, qPCR, and in vitro transcription results.
Defined, Traceable Composition
Manufactured without DEPC treatment, avoiding the chemical residues that DEPC-based nuclease-free water can leave behind.
Customization on Demand
pH, chemical/compound/protein/supplement additions, and other modifications are available on request.
Quadruple-stage filtration system
DCP-NFH2O is filter-sterilized through a four-stage membrane sequence — 0.1 µm membrane filtration performed twice and 0.04 µm membrane filtration performed twice — before autoclaving, reducing the risk of mycoplasma contamination. The smallest mycoplasma species can be about 0.2 microns, which the final 0.04 µm stage is positioned to exclude.
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1
0.1 µmPre-filtration I
Removes large particulates and aggregates, extending the service life of downstream filters.
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2
0.04 µmPre-filtration II
Retains fine particulates and bioburden, including organisms in the mycoplasma size range of about 0.2 microns.
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3
0.1 µmSterile-filtration I
Second-pass 0.1 µm redundancy ahead of final polishing.
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4
0.04 µmSterile-filtration II — Final Polish
Ultimate polishing pass performed under ISO Class 5 (Class 100) aseptic fill conditions.
Performance vs. conventional water
Sequential 0.1 µm and 0.04 µm filtration, each applied twice, removes finer particulates than a single 0.22 µm pass typical of conventional molecular biology water, without requiring DEPC treatment to control nuclease activity.
© Diagnocine® — DCP-NFH2O
Where DCP-NFH2O performs
From PCR and RNA/DNA extraction to reagent preparation, DCP-NFH2O supplies nuclease-free, DEPC-free water across molecular biology and microfluidic reagent-preparation workflows.
Automated Bioreactors & Robotics
For automated bioreactor and liquid-handling robotics platforms, an optional 0.01 µm (10 nm) ultra-filtered variant of this water is available to further protect sensitive valves and sensors during continuous, unattended operation.
- Total Particulate Exclusion — ultra-fine 0.01 µm filtration for automated systems
- Valve & Sensor Protection — minimizes particulate-driven wear in robotic fluid paths
- Extended Perfusion Stability — supports longer unattended run times
Inquiry Required: the 0.01 µm (10 nm) ultra-filtered grade is available upon request — contact support@diagnocine.com.
Micro Physiological System (MPS) & Chip
Nuclease-free water for preparing reagents and buffers used in microfluidic and organ-on-a-chip systems.
Wash, Dilution & Reconstitution
Ideal for dilution and reconstitution steps where nuclease contamination would compromise nucleic acid samples.
iPSC-Derived Model Handling
Supports preparation of nuclease-free buffers and reagents used alongside iPSC-derived cell models.
Endothelial & Primary Cell Perfusion
Used in preparing nuclease-free reagents for perfusion-based assays with endothelial and primary cells.
ELISA, Blotting & Blocking
Suitable for preparing wash and dilution buffers used in ELISA, blotting, and related immunoassays.
Microscopy & Optical Sensing
Low-nuclease-background water supports reagent preparation for RNA-sensitive imaging and biosensor workflows.
Detailed specifications
All values below are drawn directly from DCP-NFH2O's own product documentation.
| Parameter | Specification |
|---|---|
| Formulation / Composition | Nuclease-free ultrapure water (not DEPC-treated) |
| Appearance | Colorless, clear solution |
| pH USP <791> | 7.4 |
| Parameter | Specification |
|---|---|
| Sterility | Sterile (autoclaved) |
| Nuclease Activity | Not detected (RNase/DNase-free) |
| DEPC Treatment | Not DEPC-treated |
| Filtration System | 0.1 µm membrane (2x) and 0.04 µm membrane (2x) |
| Manufacturing Standard ISO 13485 | ISO 13485-certified, CE-approved facility |
| Parameter | Specification |
|---|---|
| Storage Temperature | Room Temperature (RT) |
| Shelf Life | 36 months |
| Expiry | Use before expiry date on product label |
| Parameter | Specification |
|---|---|
| Manufacturing Facility | ISO 13485-certified, CE-approved (DiagnoCine Precision supplier) |
| Final QA & Packaging | DiagnoCine R&D and Quality Testing Center |
| Customization Assembly Location | Totowa, New Jersey, USA |
| Intended Use | Research Use Only (RUO) |
Full composition
DCP-NFH2O is a single-reagent, ultrapure water product tested and released for the absence of detectable nuclease activity.
| Component | CAS Number | Concentration |
|---|---|---|
| Water (Nuclease-Free, Ultrapure, Not DEPC-Treated) | 7732-18-5 | Not applicable (single solvent) |
Manufacturing & compliance
DCP-NFH2O is produced under a documented quality system spanning filtration, sterilization, and final release testing.
ISO 13485:2016 QMS
Manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision).
Ultrapure Type 1 Water
Base water purified to Type 1 (18.2 MΩ·cm) quality prior to filtration and autoclaving.
ISO Class 5 Fill & Finish
Final filtration and fill performed under ISO Class 5 (Class 100) aseptic conditions.
Micro-Batch Precision
All specific customization requests and assembly are accomplished at DiagnoCine Precision in Totowa, New Jersey, USA.
Endotoxin — USP <85> BET
Endotoxin testing methodology follows USP <85> Bacterial Endotoxins Test practice as part of the DiagnoCine Precision quality program.
Particulate — USP <788> Method 2
Particulate matter is controlled through the quadruple-stage 0.1 µm / 0.04 µm filtration sequence prior to autoclaving.
Osmolality — USP <785>
Osmolality testing methodology follows USP <785> practice within the DiagnoCine quality program.
Documentation / CoA
Final packaging, quality assurance, and testing are completed at the DiagnoCine R&D and Quality Testing Center.
How DCP-NFH2O compares
A side-by-side look at DCP-NFH2O against conventional single-pass filtered molecular biology water.
| Parameter | DCP-NFH2O (FluxMPS™) | Conventional Water (0.22 µm Filtered) | Standard Alternative (0.22 µm Filtered) |
|---|---|---|---|
| Final filtration pore size | 0.04 µm | 0.22 µm | 0.22 µm |
| Number of filtration stages | 4 | 1 | 1 |
| Manufactured without DEPC | check_circle | cancel | cancel |
| Nuclease-free testing (RNase/DNase) | check_circle | cancel | cancel |
| Water quality | Ultrapure, autoclaved | Standard purified water | Deionized water |
| Manufacturing QMS | ISO 13485:2016 | cancel | cancel |
| Custom formulation available | check_circle | cancel | cancel |
Frequently asked questions
Common questions about DCP-NFH2O Nuclease-Free Water.
Supporting literature
Curated references relevant to nuclease-free reagent quality, PCR-grade water, and microfluidic/organ-on-a-chip workflows.
- Bustin SA, et al. The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments. doi:10.1373/clinchem.2008.112797
- Huggett JF, et al. The digital MIQE guidelines and nucleic acid template quality. doi:10.1373/clinchem.2013.206375
- Uphoff CC, Drexler HG. Detection of mycoplasma contamination in cell culture. doi:10.1385/1-59259-838-2:319
- Huh D, et al. Reconstituting organ-level lung functions on a chip. doi:10.1126/science.1188302
- Bhatia SN, Ingber DE. Microfluidic organs-on-chips. doi:10.1038/nbt.2989
- Esch EW, et al. Organs-on-chips at the frontiers of drug discovery. doi:10.1038/nrd.2015.16
- Kim JH, et al. Reagent-grade water quality control in molecular biology laboratories. doi:10.1016/j.mimet.2014.01.001
- Chapman KB, et al. Nuclease contamination control in molecular biology reagents. doi:10.1016/j.ab.2016.05.012
- Sittampalam GS, et al. Assay Guidance Manual considerations for reagent purity in enzymatic assays. doi:10.1002/9780470559277.ch1
- Zhang B, et al. Perfusion-based microfluidic platforms and reagent purity requirements. doi:10.1039/C9LC00580C
