Taq SSB (Single-Stranded DNA Binding) protein functional

Taq SSB (Single-Stranded DNA Binding) protein functional

Cat. No. FNK-02-044
Store at -20℃ 
Size 100µg


Description

Thermus aquaticus derived single-stranded DNA binding protein (SSB) is a thermostable protein which binds to single-stranded DNA with high specificity but does not bind well to double-stranded DNA (1). It plays important roles in DNA replication and recombination (2). Thermus aquaticus SSB gene was expressed in E.Coli in large quantities and the protein was highly purified. MW is 30.0 kDa, same as that of the natural protein.

Applications

Stabilizes single-stranded DNA in DNA replication, repair, and recombination

Storage conditions

50mM Tris-HCl (pH 8.0), 200mM NaCl, 0.1mM dithiothreitol, 0.5mM EDTA, 50% glycerol, Store at -20℃

Activity

 Single-stranded DNA binding activity was confirmed (Fig.2).

Concentration

0.5 mg/ml

Quality Assurance

Greater than 95% of protein determined by SDS-PAGE (CBB staining) The absence of endonucleases and exonucleases was confirmed.

Data Link 

UniProtKB/Swiss-Prot Q9KH06 (SSB_THEAQ)

References

1. Dabrowski S et al (2002) “Novel thermostable ssDNA-binding proteins from Thermus thermophilus and T. aquaticus-expression and purification.” Protein Expr Purif. 26: 131-138 PMID: 12356480
2. Greipel J et al (1989) In Saenger,W. and Heinemann,U.(eds), Protein-Nucleic Acid Interaction, Macmillan, London, pp.61-86