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Rad51, Human, Recombinant, (100µg)

Product#: FNK-10-002

$1,259.12

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Rad51, Human, Recombinant

DiagnoCine offers excellent Rad51 I Antibodies and Recombinant proteins to researchers studying DNA repair, Mitochondrial DNA synthesis, Replication Stress, Homologous Recombination, and Meiosis.

Human diseases include multiple cancer types (breast cancer, prostate cancer, renal cancer, and lung cancer & others), aging, congenital mirror movement disorder, and Fanconi anemia.

Rad51 I Antibodies have excellent quality and this highly pure antibody can be adapted for Western blotting, Immunoprecipitation, and Immunofluorescent staining, and other research protocols with optimization.

Cat. No. FNK-10-002
Size 100 µg
Store -80°C (Sent at 4℃ or with dry-ice. Store at -80℃ for long period)
Purity ＞95%(SDS-PAGE) as judged from SDS-PAGE analysis (Fig.1)
Host E. coli
Concentration1.0 mg/ml

Description

Human Rad51 protein is a functional and structural homolog of E. coli RecA protein, which plays a major role in genetic recombination and recombination repair by mediating strand exchange reaction between homologous DNA strands (1). Rad51 functionally and physically interacts with its paralogs Dmc1, Rad51B, Rad51C, Rad51D, Xrcc2 and Xrcc3, and also with Rad52 in recombination processes. It also interacts with oncogene proteins and tumor suppressors such as BRCA1, BRCA2, and P53 for the maintenance of genome stability (1).

Fig.1 Analysis of purified human Rad51 protein by SDS-PAGE

Rad51 protein was highly purified from E. coli over-expressing human Rad51 protein as a recombinant protein (Fig. 1). Since the tag was removed from the recombinant protein (it still contains Gly-Ser-His at the N-terminal), it has been shown to retain nuclear filament forming and strand-exchange activity as well as interaction with Rad52 (2). This product was confirmed to possess single strand DNA stimulated ATPase activity.

Applications

Studies on homologous recombination in mammals including human
Studies on the interaction of Rad51 protein with various proteins
To be used as a standard for Western blotting

Specification

Form 1.0 mg/ml in 20 mM Tris-HCl pH8.0, 100 mM KCl, 1 mM DTT, 0.5 mM EDTA, 10% glycerol

Data Link UniProtKB/Swiss-Prot Q06609 (RAD51_HUMAN)

References
This product was used in references 2 and 3.

Friedberg EC et al DNA Repair and Mutagenesis 2nd ed., ASM Press (2006)
Kurumizaka H et al “Human Rad51 amino acid residues required for Rad52 binding.” J Mol Biol 291: 537-548 (1999) PMID: 10448035
Murayama Y et al “Formation and branch migration of Holliday junctions mediated by eukaryotic recombinases.” Nature 451:1018-1021 (2008) PMID: 18256600