FluxMPS™ Leibovitz's L-15 Medium w/o Sodium Pyruvate, Phenol Red: 1X Liquid
FluxMPS™ Leibovitz's L-15 Medium w/o Sodium Pyruvate, Phenol Red: 1X Liquid
Microfluidics Suitable, quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) 1X liquid Leibovitz's L-15 medium engineered for microfluidic channels, organ-on-a-chip (OoC), and microphysiological systems (MPS). Manufactured under ISO 13485:2016 using Ultrapure Type 1 water (18.2 MΩ·cm) with tight trace-metal and organic-carbon (TOC) control. This formulation is bicarbonate-free and CO2-independent, using galactose (0.9 g/L) as the defined carbon source in place of glucose.
- Quadruple-stage filtration train (0.1 µm → 0.04 µm → 0.1 µm → 0.04 µm) reaching a 0.04 µm final cut-off, Sterile process
- Endotoxin release specification: < 0.05 EU/mL (LAL, USP <85> BET), tested per manufacturing batch
- Galactose-based carbon source (0.9 g/L) replacing glucose; sodium pyruvate and phenol red removed for defined metabolic control and optical clarity
- Bicarbonate-free, CO2-independent formulation suited to open-bench and closed microfluidic culture
- L-Glutamine (300 mg/L), calcium and magnesium retained for standard cell attachment and growth support
- Manufactured under an ISO 13485:2016 quality management system; final QC and packaging at Diagnocine, Totowa, NJ
- Customization of pH, galactose concentration, salts, HEPES, and nutrient composition available on request — support@diagnocine.com
- Carbon source (Galactose)0.9 g/L (900 mg/L)
- L-Glutamine300 mg/L ([+])
- Sodium PyruvateRemoved ([-])
- pH (USP <791>)7.4
- Osmolality (USP <785>)300 - 340 mOsm/kg
- Endotoxin (USP <85>)< 0.05 EU/mL
- Filtration0.1µm ×2 + 0.04µm ×2
- Storage2-8°C, protect from light
- Shelf Life12 months from date of manufacture, unopened
- ShippingCold pack (2–8°C)
Engineered where standard media fails
Conventional 0.22 µm filtered media allows mycoplasma (0.2–0.3 µm), sub-visible particulates, and microaggregates to pass freely — clogging microchannels, corrupting biosensors, and confounding metabolic assays.[1,2] FluxMPS™ closes that gap with a validated four-stage filtration train.
Microchannel-safe purity
A 0.04 µm final filter and USP <788> Method 1 particulate compliance support unobstructed flow in narrow microfluidic channels and long-duration perfusion.
Defined metabolic control
A galactose-based carbon source and controlled amino acid profile support Warburg-effect studies, glycolysis-focused research, and metabolic flux analysis.[3]
Ultrapure-grade water
Prepared with Ultrapure Type 1 water (18.2 MΩ·cm) — tightly controlled for trace metals and organic carbon (TOC) to support sensitive analytical and biosensor workflows.
Low background for imaging
An ultra-low particulate baseline supports confocal live-cell imaging, fluorescent biosensors, and automated high-content analysis on chip.
Consistent, defined formulation
A precisely defined free-amino-acid and vitamin profile, consistent with the classic Leibovitz's L-15 design, manufactured with micro-batch lot-to-lot consistency.
Customization on demand
pH, galactose concentration, HEPES, salts, and nutrients adjusted on request. Contact support@diagnocine.com.
Quadruple-stage filtration system
This lot is manufactured to a validated four-stage sequential filtration train reaching a final pore size of 0.04 µm — a repeated pre-filter and final-filter pair, run twice for full redundancy.
-
1
0.1 µm Prefiltration I
Removes large particulates, cell debris, and protein aggregates. Protects the first 0.04 µm cartridge downstream.
-
2
0.04 µm Final filtration I
First 0.04 µm pass; retains sub-micron particulates and microaggregates that pass a 0.22 µm filter.
-
3
0.1 µm Prefiltration II
A second, dedicated prefilter protecting the second 0.04 µm cartridge in the train.
-
4
0.04 µm Final filtration II — Polish
Ultimate polishing filter; aseptic fill and finish in a validated ISO Class 5 (Class 100) laminar-flow workstation.
Filtration architecture
The train is run as two dedicated prefilter-plus-final-filter pairs in series, giving full redundancy at the 0.04 µm final cut-off rather than a single descending cascade.
© Diagnocine® — DCP-LL15-PR1X
Optimized for next-generation cell biology platforms
FluxMPS™ Leibovitz's L-15 Medium w/o Sodium Pyruvate, Phenol Red: 1X Liquid is validated for applications where microchannel cleanliness, signal fidelity, and defined metabolic control are critical.
Automated Bioreactors & Robotics
For closed-loop automated bioreactors and robotic liquid-handling systems, Diagnocine also offers an optional 0.01 µm (10 nm) ultra nano-filtered MPS Grade variant of this formulation, adding a further 0.02 µm and 0.01 µm polishing stage after the 0.04 µm filtration described above.
- Total Particulate Exclusion — minimizes valve and sensor fouling in long-run automated systems
- Valve & Sensor Protection — reduces risk of microvalve occlusion in closed perfusion loops
- Extended Perfusion Stability — supports multi-week unattended culture runs
Inquiry Required: The 0.01 µm MPS Grade variant is produced to order. Contact support@diagnocine.com to request specifications and lead time.
Micro Physiological System (MPS) & Chip
Ultra-low particulate media for perfusion in organ chips, tissue chips (ToC), and body-on-a-chip (BoC) devices.[2]
Warburg Effect & Metabolic Research
Galactose-defined carbon source supports Warburg-effect studies, aerobic glycolysis, and cancer metabolomics.[3]
iPSC-Derived Models
Ultra-clean, low-endotoxin baseline minimizes non-specific signals in iPSC differentiation and functional organoid readouts.
Endothelial & Primary Cells
Particle-free, CO2-independent perfusion media for TEER measurement, endothelial monolayer integrity, and primary cell culture.
Metabolic Flux Analysis
Chemically defined, bicarbonate-free, phenol red-free formulation compatible with ¹³C metabolic tracing, Seahorse XF respiratory assays, and NMR metabolomics.
Microscopy & Optical Sensing
Ultra-low particulate, phenol red-free medium suited for confocal microscopy, fluorescent biosensors, and automated imaging on chip.[4]
Full technical specification
Every lot of FluxMPS™ Leibovitz's L-15 Medium w/o Sodium Pyruvate, Phenol Red: 1X Liquid is released against multi-parameter QC specifications.
| Parameter | Specification |
|---|---|
| Formulation | [+] L-Glutamine, Calcium, Magnesium / [-] Sodium Bicarbonate, Phenol Red, Sodium Pyruvate |
| Appearance | Colorless, clear solution (phenol red-free) |
| pH USP <791> | 7.4 |
| Osmolality USP <785> | 300 - 340 mOsm/kg |
| Carbon source (Galactose) | 0.9 g/L (900 mg/L) |
| L-Glutamine | 300 mg/L ([+]) |
| Sodium Pyruvate | Removed ([-]) |
| Phenol Red | Removed ([-]) |
| Parameter | Specification |
|---|---|
| Endotoxin USP <85> | < 0.05 EU/mL |
| Sterility USP <71> | No growth after 14 days |
| Mycoplasma | 0.1 µm mycoplasma-retentive filtration (not tested per lot) |
| Particulate ≥10 µm <788> Method 1 | Compliant |
| Particulate ≥25 µm <788> Method 1 | Compliant |
| Water purity | Ultrapure Type 1, 18.2 MΩ·cm |
| Manufacturing | ISO 13485:2016 ISO |
| Fill environment | ISO Class 5 (Class 100) |
| Parameter | Specification |
|---|---|
| Storage | 2-8°C, protect from light |
| Freeze–thaw | Do not freeze |
| Shelf life | 12 months from date of manufacture, unopened |
| Shipping | Cold pack (2–8°C) |
| CO2 requirement | Not required (CO2-independent) |
| Parameter | Specification |
|---|---|
| Raw material grade | Pharmaceutical/research grade CoA |
| Traceability | Full lot traceability; CoA per batch |
| Manufacturing QMS | ISO 13485:2016 ISO |
| UNSPSC | 41116155 — Molecular biology and cell culture growth media (UNv260801) |
| Regulatory alignment | 21 CFR Part 820 (QMSR) aligned |
| Production | Micro-batch; Totowa, NJ, USA |
| Intended use | RUO only |
Full composition (mg/L)
Every ingredient below is present in this 1X liquid formulation at the exact concentration listed. Total: 33 components across 3 tabs (Inorganic Salts, Amino Acids, Vitamins & Others). All values are per-lot verified and reported on the Certificate of Analysis (CoA).
INORGANIC SALTS
| Component | CAS Number | mg/L |
|---|---|---|
| Calcium chloride dihydrate | 10035-04-8 | 185.000 |
| Magnesium chloride hexahydrate | 200.000 | |
| Magnesium sulfate anhydrous | 7487-88-9 | 97.720 |
| Potassium chloride | 7447-40-7 | 400.000 |
| Potassium phosphate monobasic | 7778-77-0 | 60.000 |
| Sodium chloride | 7647-14-5 | 8000.000 |
| Sodium dihydrogen phosphate anhydrous | 7558-80-7 | 190.120 |
AMINO ACIDS
| Component | CAS Number | mg/L |
|---|---|---|
| L-Alanine | 56-41-7 | 225.000 |
| Glycine | 56-40-6 | 200.000 |
| L-Arginine (free base) | 500.000 | |
| L-Asparagine | 250.000 | |
| L-Cysteine (free base) | 120.000 | |
| L-Glutamine | 56-85-9 | 300.000 |
| L-Histidine (free base) | 250.000 | |
| L-Isoleucine | 73-32-5 | 250.000 |
| L-Leucine | 61-90-5 | 125.000 |
| L-Lysine hydrochloride | 657-27-2 | 94.000 |
| L-Methionine | 63-68-3 | 75.000 |
| L-Phenylalanine | 63-91-2 | 125.000 |
| L-Serine | 56-45-1 | 200.000 |
| L-Threonine | 72-19-5 | 300.000 |
| L-Tryptophan | 73-22-3 | 20.000 |
| L-Tyrosine disodium salt | 276.160 | |
| L-Valine | 72-18-4 | 100.000 |
| Component | CAS Number | mg/L |
|---|---|---|
| VITAMINS | ||
| Choline chloride | 67-48-1 | 1.000 |
| D-Ca-Pantothenate | 137-08-6 | 1.000 |
| Folic acid | 59-30-3 | 1.000 |
| Nicotinamide | 98-92-0 | 1.000 |
| Pyridoxine hydrochloride | 58-56-0 | 1.000 |
| Riboflavin-5-phosphate sodium salt | 0.100 | |
| Thiamine hydrochloride | 67-03-8 | 1.000 |
| OTHERS | ||
| i-Inositol | 87-89-8 | 2.000 |
| D-Galactose | 59-23-4 | 900.000 |
Manufacturing & compliance
Every batch is subjected to multi-parameter lot-release testing before distribution.
ISO 13485:2016 QMS
Manufactured by ISO 13485-certified suppliers. Final packaging, QA and testing at Diagnocine R&D Center; customization at Diagnocine Precision, Totowa, NJ, USA.
Ultrapure Type 1 Water
18.2 MΩ·cm, low trace-metal and organic carbon (TOC) content. Supports sensitive analytical and biosensor workflows.
ISO Class 5 Fill & Finish
Aseptic fill in a validated ISO Class 5 laminar-flow workstation, preserving the filtration gains described above in the final container.
Micro-Batch Precision
Small-batch production supporting tight lot-to-lot consistency in pH, osmolality, and endotoxin release testing — important for reproducible long-duration MPS experiments.
Endotoxin — USP <85> BET
LAL assay; assay sensitivity 0.005 EU/mL. Release specification: < 0.05 EU/mL, tested per manufacturing batch.
Particulate — USP <788> Method 1
Light-obscuration particle count. Compliant at both the ≥10 µm and ≥25 µm limits.
Osmolality — USP <785>
Freezing-point depression osmometry. Release range: 300 - 340 mOsm/kg.
Certificate of Analysis (CoA)
Full CoA per lot. Request at support@diagnocine.com with lot number.
- Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
- pH, osmolality, conductivity, appearance and clarity
- Sterility
How DCP-LL15-PR1X compares
Key differences in grade, filtration, mycoplasma control, and endotoxin release specification versus conventional 0.22 µm filtered L-15 media.
| Parameter | DCP-LL15-PR1X (FluxMPS™) | Standard L-15 (0.22 µm) | Competitor L-15 (0.22 µm) |
|---|---|---|---|
| Grade | Microfluidics Suitable | Not specified | Not specified |
| Formulation definition | [+] L-Glutamine, Calcium, Magnesium / [-] Sodium Bicarbonate, Phenol Red, Sodium Pyruvate | Standard | Standard |
| Final filtration pore size | 0.04 µm | 0.22 µm | 0.22 µm |
| Filtration stages | 4 (Quadruple) | 1 | 1 |
| Mycoplasma-retentive filtration | check_circle | cancel | cancel |
| Endotoxin (release specification) | < 0.05 EU/mL | Corning classical liquid media — < 0.25 EU/mL Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL Gibco classical DMEM — Not specified (recorded per lot) |
|
| USP <788> particulate compliance | check_circle Method 1 | cancel | cancel |
| Water quality | Ultrapure Type 1, 18.2 MΩ·cm | Unspecified | Unspecified |
| Manufacturing QMS | ISO 13485:2016 | Varies | Varies |
| Microfluidic channel compatibility | check_circle | cancel | cancel |
| Custom formulation | check_circle On request | cancel | cancel |
Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".
Frequently asked questions
Common questions about FluxMPS™ Leibovitz's L-15 Medium w/o Sodium Pyruvate, Phenol Red: 1X Liquid (DCP-LL15-PR1X).
Supporting literature
- Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
- Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
- Vander Heiden MG, et al. Understanding the Warburg effect. Science. 2009;324:1029–1033. doi:10.1126/science.1160809
- Sontheimer-Phelps A, et al. Modelling cancer in microfluidic human organs-on-chips. Nat Rev Cancer. 2019;19:65–81. doi:10.1038/s41568-018-0104-6
- van Duinen V, et al. Microfluidic 3D cell culture. Curr Opin Biotechnol. 2015;35:118–126. doi:10.1016/j.copbio.2015.05.002
- Jang KJ, et al. Reproducing human drug toxicities using a Liver-Chip. Sci Transl Med. 2019;11:eaax5516. doi:10.1126/scitranslmed.aax5516
- Kasendra M, et al. Primary human Small Intestine-on-a-Chip. Sci Rep. 2018;8:2871. doi:10.1038/s41598-018-21201-7
- Leibovitz A. The growth and maintenance of tissue-cell cultures in free gas exchange with the atmosphere. Am J Hyg. 1963;78:173–180.
- Skardal A, et al. Multi-tissue organ-on-a-chip platform. Sci Rep. 2017;7:8837. doi:10.1038/s41598-017-08879-x