FluxMPS™ Basal Medium Eagle (BME) w/o Sodium Bicarbonate: 1X Liquid
FluxMPS™ Basal Medium Eagle (BME) w/o Sodium Bicarbonate: 1X Liquid
FluxMPS™ Basal Medium Eagle (BME) w/o Sodium Bicarbonate: 1X Liquid is a Microfluidics Suitable, ultra-filtered cell culture medium engineered for organ-on-a-chip (OoC), tissue-on-a-chip (ToC), and microphysiological system (MPS) applications. A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration.
- Quadruple-stage nano-filtration: 0.1 µm (Prefiltration I & II) + 0.04 µm (Final filtration I & II — Polish), reaching a 0.04 µm final pore size across four passes
- Endotoxin release specification: < 0.05 EU/mL (USP <85> BET)
- Basal Medium Eagle (BME) w/o Sodium Bicarbonate: 1X Liquid base formulation; pH 7.4 (USP <791>)
- Formulated without sodium bicarbonate or HEPES buffer — sodium bicarbonate should be added by the end user to match the CO₂ concentration of the incubation system
- Contains 1000 mg/L D-glucose and 292 mg/L L-glutamine as primary energy and nitrogen sources
- 0.1 µm mycoplasma-retentive filtration (not tested per lot)
- Manufactured under an ISO 13485:2016 quality management system; final QC and packaging at Diagnocine, Totowa, NJ
- Custom formulations available — pH, glucose, salts, HEPES, and nutrient composition on request
- Glucose1000 mg/L (1.0 g/L)
- L-Glutamine292 mg/L
- Sodium PyruvateNot present
- HEPESNot present
- pH (USP <791>)7.4
- Osmolality (USP <785>)230 - 270 mOsm/kg H2O
- Endotoxin (USP <85>)< 0.05 EU/mL
- Filtration0.1 µm ×2 + 0.04 µm ×2
- Storage2–8°C, protect from light
- Shelf Life12 months from date of manufacture, unopened
Engineered where standard media fails
Conventional 0.22 µm-filtered media carry mycoplasma-sized particulates (0.2–0.3 µm), subvisible debris, and endotoxin variability that accumulate inside microchannels — corrupting biosensor readings and shortening device lifetimes. FluxMPS™ is designed to address these failure modes at the source.
Microchannel-safe purity
0.1 µm mycoplasma-retentive stage plus a 0.04 µm polishing filter; USP <788> Method 1 (light obscuration) particulate compliance verified per batch.
Total metabolic control
Defined glucose (1000 mg/L) and L-glutamine (292 mg/L) levels with a bicarbonate/HEPES-free base, giving you full control over buffering and carbon source for metabolic flux experiments.
Ultrapure-grade water
Prepared with Type 1 water (18.2 MΩ·cm) with tightly controlled trace-metal and total organic carbon (TOC) levels, supporting consistent culture chemistry batch to batch.
Low background for imaging
Ultra-low particulate baseline reduces background scatter for confocal microscopy, live-cell biosensors, and TEER measurements. Note: this formulation contains phenol red, which may contribute absorbance in some fluorescence-based assays.
Rich, stable nutrient profile
Micro-batch precision manufacturing locks in amino acid and vitamin concentrations, supporting lot-to-lot reproducibility for long-term perfusion studies.
Customization on demand
pH, glucose concentration, salts, HEPES, and full nutrient composition available on request. Contact support@diagnocine.com.
Quadruple-stage filtration system
FluxMPS™ Basal Medium Eagle (BME) w/o Sodium Bicarbonate: 1X Liquid is processed through a four-stage serial filtration sequence — a repeated prefilter-plus-final-filter pair, run twice — reaching a 0.04 µm final pore size that addresses mycoplasma-scale and subvisible particulates that 0.22 µm filtration cannot.
-
1
0.1 µm Prefiltration I
Removes large particulates and protein aggregates; protects the first 0.04 µm cartridge and extends filter life across microchannel-scale flow paths.
-
2
0.04 µm Final filtration I
Sub-micron particulate and mycoplasma-scale (0.2–0.3 µm) retention — a step absent in standard 0.22 µm filtration.
-
3
0.1 µm Prefiltration II
A second, dedicated prefilter protects the second 0.04 µm cartridge, giving the train full redundancy.
-
4
0.04 µm Final filtration II — Polish
Ultimate polishing step; aseptic fill performed in an ISO Class 5 (Class 100) laminar-flow workstation.
Performance vs. conventional media
By reaching a 0.04 µm final pore size across four sequential stages, FluxMPS™ delivers approximately 5× cleaner media by particulate count compared to single-pass 0.22 µm filtration, with USP <788> Method 1 compliance verified on every production batch.
© Diagnocine® — DCP-BME-B1X
Designed for next-generation cell culture platforms
FluxMPS™ Basal Medium Eagle (BME) w/o Sodium Bicarbonate: 1X Liquid is a Microfluidics Suitable medium validated for use across organ-on-a-chip, metabolic research, live-cell imaging, and primary cell models where particulate contamination and endotoxin variation are unacceptable.
Automated Bioreactors & Robotics
An optional 0.01 µm (10 nm) MPS Grade variant, using a separate six-stage ultra nano-filtration cascade, is available for automated bioreactor and robotic perfusion systems requiring the ultimate particulate exclusion.
- Total Particulate Exclusion: 10 nm filtration for nanoscale valve and sensor protection
- Valve & Sensor Protection: reduces risk of particulate-induced blockage in precision fluidic systems
- Extended Perfusion Stability: supports flow-rate consistency across multi-week automated runs
Inquiry Required: The 0.01 µm MPS Grade variant is available by special order. Contact support@diagnocine.com to request this variant.
Micro Physiological System (MPS) & Chip
Ultra-filtered formulation reduces the risk of microchannel clogging and helps maintain laminar flow integrity across complex chip geometries.
Warburg Effect & Metabolic Research
Defined 1000 mg/L glucose and a low-endotoxin background support metabolic flux analysis and Warburg effect studies.
iPSC-Derived Models
Ultrapure formulation supports sensitive iPSC differentiation protocols where endotoxin and particulates cause off-target effects.
Endothelial & Primary Cells
Microchannel-safe purity supports endothelial barrier integrity and TEER measurement in perfusion models.
Metabolic Flux Analysis
Chemically defined base supports isotope tracing and flux analysis experiments. Not compatible with Agilent Seahorse XF assays, which require bicarbonate-free, phenol red-free medium — this formulation contains phenol red.
Microscopy & Optical Sensing
Ultra-low particulate load supports high-content confocal imaging and optical biosensor integration. Note: phenol red content may contribute background absorbance in some fluorescence-based assays.
Batch-release quality parameters
Every production batch of FluxMPS™ Basal Medium Eagle (BME) w/o Sodium Bicarbonate: 1X Liquid undergoes the complete quality-release battery listed below before shipment.
| Parameter | Specification |
|---|---|
| Formulation | Contains L-Glutamine, Phenol Red, Calcium, Magnesium and Glucose; without Sodium Bicarbonate, HEPES and Sodium Pyruvate |
| Appearance | Red-colored, clear solution |
| pH USP <791> | 7.4 |
| Osmolality USP <785> | 230 - 270 mOsm/kg H2O |
| Glucose | 1000 mg/L (1.0 g/L) |
| L-Glutamine | 292 mg/L |
| Sodium Pyruvate | Not present |
| Phenol Red | Present |
| Parameter | Specification |
|---|---|
| Endotoxin USP <85> BET | < 0.05 EU/mL (batch release specification — see §Manufacturing) |
| Sterility USP <71> | No growth after 14 days |
| Mycoplasma | 0.1 µm mycoplasma-retentive filtration (not tested per lot) |
| Particulate ≥10 µm USP <788> Method 1 | Compliant |
| Particulate ≥25 µm USP <788> Method 1 | Compliant |
| Water Purity | Ultrapure Type 1, 18.2 MΩ·cm |
| Manufacturing std. | ISO 13485:2016 ISO 13485 |
| Fill environment | ISO Class 5 (Class 100) |
| Parameter | Specification |
|---|---|
| Storage temperature | 2–8°C, protected from light |
| Freeze-thaw | Not recommended |
| Shelf life | 12 months from date of manufacture, unopened |
| Shipping condition | Cold pack |
| CO₂ requirement | Formulated without sodium bicarbonate or HEPES buffer. Sodium bicarbonate should be added by the end user at a concentration matched to the CO₂ level of the incubation system (typically 1.5–3.7 g/L NaHCO3 for 5–10% CO₂). |
| Parameter | Specification |
|---|---|
| Raw material grade | Cell culture / reagent grade |
| Traceability | Full lot documentation, CoA available |
| Manufacturing QMS | ISO 13485:2016 certified |
| UNSPSC | 41116155 — Molecular biology and cell culture growth media (UNv260801) |
| Regulatory alignment | 21 CFR Part 820 (QMSR) aligned |
| Production method | Micro-batch precision manufacturing |
| Intended use | For Research Use Only (RUO) |
Full composition (mg/L)
Complete formulation with CAS numbers. All ingredient names and mg/L values are reproduced from the manufacturer specification. Custom compositions available on request.
| Component | CAS Number | mg/L |
|---|---|---|
| INORGANIC SALTS | ||
| Calcium chloride dihydrate | 10035-04-8 | 265.000 |
| Magnesium sulfate anhydrous | 7487-88-9 | 97.720 |
| Potassium chloride | 7447-40-7 | 400.000 |
| Sodium chloride | 7647-14-5 | 6800.000 |
| Sodium dihydrogen phosphate anhydrous | 7558-80-7 | 122.000 |
| Component | CAS Number | mg/L |
|---|---|---|
| AMINO ACIDS | ||
| L-Arginine hydrochloride | 1119-34-2 | 21.100 |
| L-Cystine dihydrochloride | 30189-89-0 | 15.650 |
| L-Glutamine | 56-85-9 | 292.000 |
| L-Histidine hydrochloride | 1007-42-7 | 10.500 |
| L-Isoleucine | 73-32-5 | 26.200 |
| L-Leucine | 61-90-5 | 26.200 |
| L-Lysine hydrochloride | 657-27-2 | 36.480 |
| L-Methionine | 63-68-3 | 7.500 |
| L-Phenylalanine | 63-91-2 | 16.500 |
| L-Threonine | 72-19-5 | 23.800 |
| L-Tryptophan | 73-22-3 | 4.000 |
| L-Tyrosine disodium salt | 69847-45-6 | 25.950 |
| L-Valine | 72-18-4 | 23.400 |
| Component | CAS Number | mg/L |
|---|---|---|
| VITAMINS | ||
| Choline chloride | 67-48-1 | 1.000 |
| D-Biotin | 58-85-5 | 1.000 |
| D-Ca-Pantothenate | 137-08-6 | 1.000 |
| Folic acid | 59-30-3 | 1.000 |
| Nicotinamide | 98-92-0 | 1.000 |
| Pyridoxal hydrochloride | 65-22-5 | 1.000 |
| Riboflavin | 83-88-5 | 0.100 |
| Thiamine hydrochloride | 67-03-8 | 1.000 |
| OTHERS | ||
| D-Glucose | 50-99-7 | 1000.000 |
| Phenol red sodium salt | 34487-61-1 | 11.000 |
| i-Inositol | 87-89-8 | 2.000 |
ISO 13485:2016 manufacturing & compliance
FluxMPS™ Basal Medium Eagle (BME) w/o Sodium Bicarbonate: 1X Liquid is manufactured under a full ISO 13485:2016 quality management system, with final packaging, testing, and customization completed at Diagnocine, Totowa, New Jersey, USA.
ISO 13485:2016 QMS
Full quality management system certification covering all manufacturing, testing, and release processes for every production batch.
Ultrapure Type 1 Water
All media prepared with 18.2 MΩ·cm resistivity Type 1 water, with trace-metal and total organic carbon (TOC) control to minimize chemical background in culture chemistry.
ISO Class 5 Fill & Finish
Aseptic filling performed in validated laminar-flow (ISO Class 5 / Class 100) workstations; 21 CFR Part 820 (QMSR) aligned.
Micro-Batch Precision
Small-batch manufacturing supports lot-to-lot nutrient consistency for reproducible perfusion studies and long-term OoC experiments.
- Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
- pH, osmolality, conductivity, appearance and clarity
- Sterility
Endotoxin — USP <85> BET
LAL assay performed per batch. Release specification: < 0.05 EU/mL.
Particulate — USP <788> Method 1
Light obscuration particle count test confirms ≥10 µm and ≥25 µm particulate compliance on every batch.
Osmolality — USP <785>
Freezing-point osmometry performed per USP <785>. Result: 230 - 270 mOsm/kg H2O.
Documentation — CoA & Full Lot Records
Certificate of Analysis available for every batch, including full QC panel, raw material traceability, and release signatures.
How DCP-BME-B1X (FluxMPS™) compares
Side-by-side comparison against conventional 0.22 µm-filtered alternatives of the same base formulation.
| Parameter | DCP-BME-B1X (FluxMPS™) | Conventional BME (0.22 µm) | Standard BME alternative |
|---|---|---|---|
| Grade | Microfluidics Suitable | Not specified | Not specified |
| Formulation | Contains L-Glutamine, Phenol Red, Calcium, Magnesium and Glucose; without Sodium Bicarbonate, HEPES and Sodium Pyruvate | BME Standard | BME Equivalent |
| Final filtration pore size | 0.04 µm | 0.22 µm | 0.22 µm |
| Number of filtration stages | 4 stages | 1 stage | 1–2 stages |
| Mycoplasma barrier filtration | check_circle | cancel | cancel |
| Endotoxin (release specification) | FluxMPS™ — < 0.05 EU/mL | Corning classical liquid media — < 0.25 EU/mL Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL Gibco classical DMEM — Not specified (recorded per lot) |
|
| USP particulate compliance | check_circle USP <788> Method 1 | cancel | cancel |
| Water quality | Ultrapure Type 1 (18.2 MΩ) | Purified water | Purified water |
| Manufacturing QMS | ISO 13485:2016 | Variable | Variable |
| Microfluidic channel compatibility | check_circle Validated | cancel Risk of clogging | cancel Risk of clogging |
| Custom formulation | check_circle On request | cancel | Limited |
Comparison figures from published supplier specifications, accessed 2 September 2026. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".
Frequently asked questions
Common questions about FluxMPS™ Basal Medium Eagle (BME) w/o Sodium Bicarbonate: 1X Liquid and Microfluidics Suitable cell culture media.
Supporting literature
Peer-reviewed publications supporting the scientific rationale for Microfluidics Suitable ultra-filtered media and microfluidic cell culture applications.
- Huh D et al. (2010). Reconstituting organ-level lung functions on a chip. Science, 328(5986), 1662–1668. doi:10.1126/science.1188302
- Bhatia SN & Ingber DE (2014). Microfluidic organs-on-chips. Nature Biotechnology, 32(8), 760–772. doi:10.1038/nbt.2989
- Bhattacharya S et al. (2018). Challenges in maintaining cell viability during microfluidic experiments. Electrophoresis, 39(7), 997–1006. doi:10.1002/elps.201700375
- Warburg O (1956). On the origin of cancer cells. Science, 123(3191), 309–314. doi:10.1126/science.123.3191.309
- Emmons EV (1965). Detection of mycoplasma in cell cultures using filtration. Proceedings of the Society for Experimental Biology, 118, 1010–1015. doi:10.3181/00379727-118-29988
- Kim S et al. (2012). Gut-on-a-chip microdevice replicates key functional features of the human intestine. Lab on a Chip, 12(12), 2165–2174. doi:10.1039/c2lc40074j
- Zhang YS et al. (2017). Multisensor-integrated organs-on-chips platform for automated and continual in situ monitoring of organoid behaviors. PNAS, 114(12), E2293–E2302. doi:10.1073/pnas.1612906114
- Vernetti L et al. (2017). Functional coupling of human microphysiology systems. Scientific Reports, 7, 42296. doi:10.1038/srep42296
- Schuster B et al. (2020). Automated microfluidic platform for dynamic and combinatorial drug screening of tumor organoids. Nature Communications, 11, 5271. doi:10.1038/s41467-020-19058-4
- Zheng F et al. (2021). Organ-on-a-chip systems: microengineering to biomimic living systems. Small, 17(7), 2004175. doi:10.1002/smll.202004175
