ICG Amine
Product#: POE1616
$3,212.00
Size of product (mg)
-
1 mg
-
5 mg
-
25 mg
Availability:
Ships in 1-2 Weeks
ICG Amine
Cat. No. List below
Description
ICG Amine is a versatile near-infrared (NIR) fluorescent dye that plays a crucial role in bioimaging applications. This inactive form of indocyanine green (ICG) offers unique properties that make it valuable for researchers across various scientific disciplines.
A primary amine is attached to the ICG fluorophore through a spacer and the functional group is an amine. The primary amine group of ICG amine allows for various chemical modifications like amide bond formation with carboxylic acids, reaction with activated esters or anhydrides, schiff base formation with aldehydes, and conjugation to NHS-activated molecules. These modifications can be used to create targeted imaging probes, attach the dye to nanoparticles, or alter its pharmacokinetic properties.
The amine group on ICG Amine offers several advantages over other ICG derivatives:
1. Increased reactivity compared to carboxylic acid derivatives
2. Potential for pH-responsive behavior in certain applications
3. Ability to form stable conjugates with a wide range of biomolecules
ICG is FDA-approved for certain medical diagnostics, which can facilitate its use in translational research. Some key applications include angiography and vascular imaging, lymph node mapping in cancer staging, tumor detection and margin assessment, perfusion studies in various organs, and ophthalmic imaging, particularly for retinal angiography.
ICG Amine is a powerful tool for researchers in fields ranging from molecular biology to clinical diagnostics. Its unique combination of NIR fluorescence, versatile conjugation chemistry, and potential for chemical modifications makes it an excellent choice for developing targeted imaging probes and studying biological processes in deep tissues. The amine functionality provides additional flexibility in conjugation strategies, allowing researchers to create novel fluorescent probes for a wide range of applications. Whether used as a standalone fluorophore or as a building block for more complex imaging agents, ICG Amine continues to play a crucial role in advancing our understanding of biological systems through fluorescence imaging techniques.
What are the advantages:
1. Deep Tissue Imaging: The NIR fluorescence of ICG Amine enables deep tissue imaging, penetrating further than visible light fluorophores. This property allows researchers to visualize structures and processes beneath the skin surface, making it invaluable for in vivo imaging studies.
2. Versatile Conjugation: ICG Amine can be coupled with carboxylic acids on small molecules or biomolecules using standard amide bond coupling conditions. This versatility allows for the creation of custom fluorescent probes tailored to specific research needs.
3. Reference Standard: The compound can serve as a reference standard for dye-conjugates, providing a basis for comparison in various experimental setups.
4. Excellent Optical Properties: ICG Amine can be excited using 750-800 nm laser lines or LEDs, offering flexibility in imaging setups. Its high extinction coefficient ensures strong fluorescence signals, even at low concentrations.
A primary amine is attached to the ICG fluorophore through a spacer and the functional group is an amine. The primary amine group of ICG amine allows for various chemical modifications like amide bond formation with carboxylic acids, reaction with activated esters or anhydrides, schiff base formation with aldehydes, and conjugation to NHS-activated molecules. These modifications can be used to create targeted imaging probes, attach the dye to nanoparticles, or alter its pharmacokinetic properties.
The amine group on ICG Amine offers several advantages over other ICG derivatives:
1. Increased reactivity compared to carboxylic acid derivatives
2. Potential for pH-responsive behavior in certain applications
3. Ability to form stable conjugates with a wide range of biomolecules
ICG is FDA-approved for certain medical diagnostics, which can facilitate its use in translational research. Some key applications include angiography and vascular imaging, lymph node mapping in cancer staging, tumor detection and margin assessment, perfusion studies in various organs, and ophthalmic imaging, particularly for retinal angiography.
ICG Amine is a powerful tool for researchers in fields ranging from molecular biology to clinical diagnostics. Its unique combination of NIR fluorescence, versatile conjugation chemistry, and potential for chemical modifications makes it an excellent choice for developing targeted imaging probes and studying biological processes in deep tissues. The amine functionality provides additional flexibility in conjugation strategies, allowing researchers to create novel fluorescent probes for a wide range of applications. Whether used as a standalone fluorophore or as a building block for more complex imaging agents, ICG Amine continues to play a crucial role in advancing our understanding of biological systems through fluorescence imaging techniques.
What are the advantages:
1. Deep Tissue Imaging: The NIR fluorescence of ICG Amine enables deep tissue imaging, penetrating further than visible light fluorophores. This property allows researchers to visualize structures and processes beneath the skin surface, making it invaluable for in vivo imaging studies.
2. Versatile Conjugation: ICG Amine can be coupled with carboxylic acids on small molecules or biomolecules using standard amide bond coupling conditions. This versatility allows for the creation of custom fluorescent probes tailored to specific research needs.
3. Reference Standard: The compound can serve as a reference standard for dye-conjugates, providing a basis for comparison in various experimental setups.
4. Excellent Optical Properties: ICG Amine can be excited using 750-800 nm laser lines or LEDs, offering flexibility in imaging setups. Its high extinction coefficient ensures strong fluorescence signals, even at low concentrations.
Specifications
- Fluorophore: ICG
- Functional group: Amine
- Excitation/Emission Max.(nm): 785/812
- Extinction coefficient: ≥ 155,000 cm-1M-1
- CF280: 0.05
- Appearance: Green Solid
- Molecular Weight: 773.04 g/mol
- Solubility: DMF, DMSO
- Storage conditions: 4 ℃, protect from light
| Quick link (Cat.#) | Series | Quick link (Cat.#) | Series |
| RFP0815 | ICG | RFP0915 | ICG (water-soluble) |
| POS1604 | ICG NHS ester | POA1616 | ICG Vinylsulfone |
| POSN1604 | ICG Sulfo-NHS ester | POI1616 | ICG Isothiocyanate |
| POC1616 | ICG Carboxylic acid | PWM1301 | ICG Maleimid |
| POE1616 | ICG Amine | PWT1301 | ICG Thiol |
| POR2616 | ICG Dichlorotriazine | POH1616 | ICG Hydrazide |
| POK1616 | ICG Alkyne | POG1616 | ICG PEG4-Alkyne |
| DOC1061 | ICG ADIBO | POZ1616 | ICG Azide |
Background
ICG Dyes
ICG is clinically approved NIR dye and used in medical diagnostics, in vitro, vivo and animal model study. NIR fluorescence allows to observe the deep image from the surface of skin and its usage spreads to a wide range of research fields. ICG fluorescence method is safe and cost effective technique and used in a wide range of medical fields such as breast cancer sentinel lymph node navigation, detecting cerebral vessels, coronary arteries and biliary trees, tracking tumor location, detecting small HCC, etc. The method has several advantages such as radiation free, compact instrumentation, real-time monitoring, easy operation, etc. The maximum excitation/emission values of ICG are 785/821 nm. When ICG is injected into a human body, it rapidly bound to plasma protein, mainly high-density lipoprotein, and generates red-shifted fluorescence (845 nm). ICG in aqueous solution is unstable over time, thus the fresh solution should be used for effective trials. BioActs provides ICG dye for biological research and medical diagnostics and also offers various reactive and functionalized ICG dyes for labeling of antibodies, peptides, proteins and ligands.
Table 1. ICG dye applications
Figure 1. Structure of Indocyanine Green (ICG) dye
Citation & Reference
1. Masashi Gotoh. Development of a canine model of pulmonary emphysema and imaging of the emphysematous lung with infrared thoracoscopy. J Thorac Cardiovasc Surg 126.6 (2003): 1916-21.
2. Aaron M. Mohs. An integrated widefield imaging and spectroscopy system for contrast-enhanced, image-guided resection of tumors. IEEE Trans Biomed Eng 62.5 (2015): 1416-24.
3. Mohammed Hassan. Near Infrared Fluorescence Imaging with ICG in TECAB Surgery Using the da Vinci Si Surgical System in a Canine Model. J Card Surg 27.2 (2012): 158-162.
4. R. C. Benson. Fluorescence properties of indocyanine green as related to angiography. Phys Med Biol 23.1 (1978): 159-63.
5. Mitsuharu Miwa. The Principle of ICG Fluorescence Method. The Open Surgical Oncology Journal 2 (2010): 26-28.
