ICG Maleimide

Product#: PWM1301

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ICG Maleimide

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ICG Maleimide is thiol reactive near infrared (NIR) fluorescent dye and used to generate a stable fluorescence signal in bioimaging. NIR fluorescence allows to observe the deep image from the surface of skin and being utilized in a wide range of research fields. The maxima of Ex/Em values are at 785/812 nm. ICG might be excited using 750-800 nm laser line or LED and displays excellent optical property. Maleimides selectively label thiols of cysteine residue via 1,4-addition pathway, without interacting with amines, to form thioether linkage. Maleimides apparently do not react with methionine, histidine or tyrosine, but they might react with primary amines under strong basic environment. We offer ICG maleimide for labeling of antibodies, peptides, proteins, ligands, and amplification substrates optimized for cellular labeling and NIR imaging.    
  • Fluorophore: ICG
  • Reactive group: Maleimide
  • Excitation/Emission Max.(nm): 785/812
  • Extinction coefficient: ≥ 218,000 cm-1M-1
  • CF280: 0.05
  • Appearance: Green Solid
  • Molecular Weight: 853.08 g/mol
  • Solubility: DMF, DMSO
  • Storage conditions: -20 ℃, protect from light
 ICG Dyes
Quick link (Cat.#) Series Quick link (Cat.#) Series
RFP0815 ICG RFP0915 ICG (water-soluble)
POS1604 ICG NHS ester POA1616 ICG Vinylsulfone
POSN1604 ICG Sulfo-NHS ester POI1616 ICG Isothiocyanate
POC1616 ICG Carboxylic acid PWM1301 ICG Maleimid
POE1616 ICG Amine PWT1301 ICG Thiol
POR2616 ICG Dichlorotriazine POH1616 ICG Hydrazide
POK1616 ICG Alkyne POG1616 ICG PEG4-Alkyne


ICG Dyes

ICG is clinically approved NIR dye and used in medical diagnostics, in vitro, vivo and animal model study. NIR fluorescence allows to observe the deep image from the surface of skin and its usage spreads to a wide range of research fields. ICG fluorescence method is safe and cost effective technique and used in a wide range of medical fields such as breast cancer sentinel lymph node navigation, detecting cerebral vessels, coronary arteries and biliary trees, tracking tumor location, detecting small HCC, etc. The method has several advantages such as radiation free, compact instrumentation, real-time monitoring, easy operation, etc. The maximum excitation/emission values of ICG are 785/821 nm. When ICG is injected into a human body, it rapidly bound to plasma protein, mainly high-density lipoprotein, and generates red-shifted fluorescence (845 nm). ICG in aqueous solution is unstable over time, thus the fresh solution should be used for effective trials. BioActs provides ICG dye for biological research and medical diagnostics and also offers various reactive and functionalized ICG dyes for labeling of antibodies, peptides, proteins and ligands.

Table 1.  ICG dye applications


Figure 1Structure of Indocyanine Green (ICG) dye

Citation & Reference

1. Masashi Gotoh. Development of a canine model of pulmonary emphysema and imaging of the emphysematous lung with infrared thoracoscopy. J Thorac Cardiovasc Surg 126.6 (2003): 1916-21.

2. Aaron M. Mohs. An integrated widefield imaging and spectroscopy system for contrast-enhanced, image-guided resection of tumors. IEEE Trans Biomed Eng 62.5 (2015): 1416-24.

3. Mohammed Hassan. Near Infrared Fluorescence Imaging with ICG in TECAB Surgery Using the da Vinci Si Surgical System in a Canine Model. J Card Surg 27.2 (2012): 158-162.

4. R. C. Benson. Fluorescence properties of indocyanine green as related to angiography. Phys Med Biol 23.1 (1978): 159-63.

5. Mitsuharu Miwa. The Principle of ICG Fluorescence Method. The Open Surgical Oncology Journal 2 (2010): 26-28.


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