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- FluxMPS™ Dulbecco’s Modified Eagle Medium (DMEM), High Glucose & 50mM HEPES w/o L-Glutamine: 2X Liquid
FluxMPS™ Dulbecco’s Modified Eagle Medium (DMEM), High Glucose & 50mM HEPES w/o L-Glutamine: 2X Liquid
FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM), High Glucose & 50mM HEPES w/o L-Glutamine: 2X Liquid
Double-strength (2X) DMEM High Glucose with 50 mM HEPES — 9.0 g/L glucose and 50 mM HEPES in the concentrate, 4.5 g/L and 25 mM at 1X — for soft-agar, collagen-gel, hydrogel and other 3D or semisolid cultures, ultra-filtered through a quadruple-stage 0.1 µm / 0.04 µm filtration system. With a sodium bicarbonate + 50 mM HEPES dual buffer, it is approximately 5× cleaner than conventional 0.22 µm-filtered media by particulate count.
- 2X concentrate: mix with an equal volume of a compatible solution or matrix for a final 1X medium
- Quadruple-stage filtration: 0.1 µm membrane filtered twice, 0.04 µm membrane filtered twice
- 0.04 µm nano-filtration final polish acts as a mycoplasma barrier
- 50 mM HEPES (25 mM at 1X) for CO₂-independent pH stability during matrix preparation and handling
- Endotoxin < 0.05 EU/mL (USP <85> BET)
- Formulation: [+] 9.0 g/L Glucose, [+] Sodium Pyruvate, [+] Sodium Bicarbonate, [+] Phenol Red, [+] 50mM HEPES; [−] L-Glutamine
- Ultrapure Type 1 water (18.2 MΩ·cm); ISO Class 5 (Class 100) aseptic fill in Totowa, NJ
- Custom formulations available — pH, glucose, HEPES, salts, and nutrient composition on request
- D-Glucose9,000 mg/L
- L-GlutamineNot included
- Sodium Pyruvate220 mg/L
- BufferNaHCO₃ 7,400 mg/L + 50 mM HEPES
- Phenol Red31.8 mg/L
- AppearanceRed
- pH7.4 (± 0.04)
- Endotoxin< 0.05 EU/mL
- Filtration0.1 µm ×2 + 0.04 µm ×2
- Format2X liquid, 500 mL
Engineered where standard media fails
Conventional DMEM is sterile-filtered once at 0.22 µm. That pore size can pass mycoplasma and leaves subvisible particulates that settle in microchannels, foul membranes and hydrogels, and add noise to optical readouts.[11,12] FluxMPS™ DMEM keeps Dulbecco's formulation and removes that failure mode.
Microchannel-safe purity
A 0.04 µm final filter and USP <788> particulate control keep subvisible debris out of chips, tubing, membranes and matrices.
Total metabolic control
Defined 9,000 mg/L D-glucose, no L-glutamine and 220 mg/L sodium pyruvate in the 2X (half that at 1X).
Ultrapure-grade water
Made with Ultrapure Type 1 water (18.2 MΩ·cm) and released at < 0.05 EU/mL endotoxin by USP <85>.
Low background for imaging
Particle-depleted medium reduces scatter and debris artifacts in colony counting, confocal imaging and on-chip sensing.
Matrix-ready 2X strength
33 components at double strength, so agar, collagen, hydrogels or treatment solutions can be added 1:1 without diluting the medium below 1X.
Customization on demand
pH, glucose, HEPES, salts, and nutrient composition on request — contact support@diagnocine.com.
Quadruple-stage filtration system
Every lot of DCP-DMEMH-Q2X is membrane filtered at 0.1 µm twice and 0.04 µm twice — a ready-to-dilute 2X DMEM polished to a 0.04 µm final pore size, a purity level single-pass 0.22 µm media cannot reach.
-
01
0.1 µmPre-filtration I
Removes large particulates and aggregates and extends the life of the downstream filters.
-
02
0.04 µmPre-filtration II
Retains fine particulates, bacteria and mycoplasma.
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03
0.1 µmSterile-filtration I
Second-pass redundancy that catches anything shed or bypassed upstream.
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04
0.04 µmSterile-filtration II — Final Polish
Final nano-polish immediately before ISO Class 5 aseptic fill.
Performance vs. conventional media
By particulate count, FluxMPS™ DMEM is approximately 5× cleaner than conventional 0.22 µm-filtered media, lowering clog risk in narrow channels and long perfusion runs.
© Diagnocine® — DCP-DMEMH-Q2X
Where FluxMPS™ DMEM fits
DMEM High Glucose is a widely used basal medium containing 4.5 g/L glucose at its 1X working concentration.[1,2] It supports many primary and established mammalian cell types, including fibroblasts, neurons, glial cells, endothelial cells, smooth-muscle cells, HEK 293, HeLa, COS-7, and other normal or transformed cell lines.[3] It is commonly used in cell-growth, transfection, recombinant-protein, tissue-engineering, drug-discovery, and cell-signaling research.
DMEM High Glucose 2X is a double-strength formulation containing 9.0 g/L glucose in the concentrate and providing 4.5 g/L glucose after dilution to 1X. It is designed to be mixed with an equal volume of a compatible solution or matrix while maintaining the intended final 1X concentration of glucose, amino acids, vitamins, and salts.
Researchers use the 2X formulation to prepare customized media and cultures containing large volumes of agar, agarose, methylcellulose, collagen, hydrogels, supplements, treatment solutions, or other aqueous components. It is particularly useful for soft-agar colony assays, collagen-gel contraction studies, semisolid cultures, 3D cell culture, stem-cell research, tissue engineering, and other workflows in which adding a matrix or solution would dilute conventional 1X DMEM.[4,5]
Automated Bioreactors & Robotics
For automated bioreactors, liquid-handling robots and closed perfusion loops with fine valves and in-line sensors, an optional 10 nm (0.01 µm) ultra-filtered grade of this medium is available.
- Total Particulate Exclusion: 0.01 µm filtration removes virtually all particulates down to the nanometer scale.
- Valve & Sensor Protection: keeps micro-valves, pumps and optical or electrochemical sensors free of deposits.
- Extended Perfusion Stability: supports long, uninterrupted perfusion runs with fewer line changes.
Inquiry Required: the 0.01 µm grade is made on request — email support@diagnocine.com with your catalog number and volume.
Soft-Agar Colony Assays
Mix 1:1 with molten agar or agarose for anchorage-independent growth assays at 1X medium strength.[4]
Collagen-Gel & Hydrogel Cultures
Fold in collagen, methylcellulose or hydrogels for gel-contraction studies and 3D constructs.[5]
Semisolid & Stem-Cell Workflows
A double-strength base for methylcellulose and other semisolid cultures used in stem- and progenitor-cell research.
Fibroblasts, HEK 293 & HeLa
Supports primary and established lines for growth, transfection and recombinant-protein work after dilution to 1X.[3]
Organ-on-a-Chip & MPS
Microchannel-safe medium for perfused organ-, tissue- and body-on-a-chip platforms.[8,9,10]
Microscopy & Optical Sensing
Fewer particles means cleaner fields for colony counting, confocal imaging and on-chip biosensors.
Specifications & quality control
Release specifications for DCP-DMEMH-Q2X. Lot-specific values are reported on the Certificate of Analysis.
| Parameter | Specification |
|---|---|
| Formulation | [+] 9.0 g/L Glucose, [+] Sodium Pyruvate, [+] Sodium Bicarbonate, [+] Phenol Red, [+] 50mM HEPES; [−] L-Glutamine |
| Concentration | 2X (dilute 1:1 to 1X) |
| Appearance | Red |
| pH | 7.4 (± 0.04) USP <791> |
| Osmolality | Measured per lot; reported on CoA USP <785> |
| D-Glucose | 9,000 mg/L (9.0 g/L; 4.5 g/L at 1X) |
| L-Glutamine | Not included |
| Sodium Pyruvate | 220 mg/L |
| Sodium Bicarbonate | 7,400 mg/L |
| Phenol Red | 31.8 mg/L |
| HEPES | 11,916 mg/L (50 mM; 25 mM at 1X) |
| Parameter | Specification |
|---|---|
| Endotoxin | < 0.05 EU/mL USP <85> BET |
| Sterility | No bacterial or fungal growth after 14 days of incubation USP <71> |
| Mycoplasma | 0.04 µm barrier filtration USP <63> equiv. |
| Particulate ≥10 µm | Meets limits USP <788> M2 |
| Particulate ≥25 µm | Meets limits USP <788> M2 |
| Water purity | Ultrapure Type 1, 18.2 MΩ·cm |
| Manufacturing std. | ISO 13485:2016 ISO |
| Fill environment | ISO Class 5 (Class 100) ISO |
| Parameter | Specification |
|---|---|
| Storage temperature | 2–8 °C, protected from light |
| Freeze-thaw | Do not freeze |
| Shelf life | 12 months; use before the expiry date on the product label |
| Shipping condition | Ice Pack |
| CO₂ requirement | CO₂-controlled incubator recommended after dilution to 1X (sodium bicarbonate 7,400 mg/L in the 2X, 3,700 mg/L at 1X); 50 mM HEPES (25 mM at 1X) steadies pH during handling outside the incubator |
| Parameter | Specification |
|---|---|
| Raw material grade | High-purity, cell-culture-tested components |
| Traceability | Lot-traceable; CoA per lot |
| Manufacturing QMS | ISO 13485:2016 ISO |
| Regulatory alignment | 21 CFR Part 820 (cGMP) aligned |
| Production method | Micro-batch 2X liquid, quadruple-stage filtered; aseptic fill and finish in Totowa, NJ |
| Intended use | For Research Use Only (RUO) |
Full composition (mg/L)
DCP-DMEMH-Q2X is 2X DMEM with 9.0 g/L glucose, sodium pyruvate, sodium bicarbonate, Phenol Red and 50 mM HEPES. It does not contain L-glutamine. Users are advised to review the literature for recommendations regarding medium supplementation and physiological growth requirements specific for different cell lines. All 33 components are listed below at 2X strength; values are per-lot release basis and are halved after 1:1 dilution.
| Component | CAS Number | mg/L |
|---|---|---|
| INORGANIC SALTS | ||
| Calcium chloride dihydrate | 10035-04-8 | 530.000 |
| Ferric nitrate nonahydrate | 7782-61-8 | 0.200 |
| Magnesium sulphate anhydrous | 7487-88-9 | 195.440 |
| Potassium chloride | 7447-40-7 | 800.000 |
| Sodium bicarbonate | 144-55-8 | 7400.000 |
| Sodium chloride | 7647-14-5 | 12800.000 |
| Sodium dihydrogen phosphate anhydrous | 7558-80-7 | 218.000 |
| Component | CAS Number | mg/L |
|---|---|---|
| AMINO ACIDS | ||
| Glycine | 56-40-6 | 60.000 |
| L-Arginine hydrochloride | 1119-34-2 | 168.000 |
| L-Cystine dihydrochloride | 30925-07-6 | 125.140 |
| L-Histidine hydrochloride monohydrate | 5934-29-2 | 84.000 |
| L-Isoleucine | 73-32-5 | 210.000 |
| L-Leucine | 61-90-5 | 210.000 |
| L-Lysine hydrochloride | 657-27-2 | 292.000 |
| L-Methionine | 63-68-3 | 60.000 |
| L-Phenylalanine | 63-91-2 | 132.000 |
| L-Serine | 56-45-1 | 84.000 |
| L-Threonine | 72-19-5 | 190.00 |
| L-Tryptophan | 73-22-3 | 32.000 |
| L-Tyrosine disodium salt | 69847-45-6 | 207.580 |
| L-Valine | 72-18-4 | 188.00 |
| Component | CAS Number | mg/L |
|---|---|---|
| VITAMINS | ||
| Choline chloride | 67-48-1 | 8.000 |
| D-Ca-Pantothenate | 137-08-6 | 8.000 |
| Folic acid | 59-30-3 | 8.000 |
| Nicotinamide | 98-92-0 | 8.000 |
| Pyridoxine hydrochloride | 58-56-0 | 8.000 |
| Riboflavin | 83-88-5 | 0.800 |
| Thiamine hydrochloride | 67-03-8 | 8.000 |
| i-Inositol | 87-89-8 | 14.400 |
| OTHERS | ||
| D-Glucose | 50-99-7 | 9000.000 |
| Phenol Red | 34487-61-1 | 31.800 |
| Sodium pyruvate | 113-24-6 | 220.000 |
| HEPES | 7365-45-9 | 11916.000 |
Manufacturing & compliance
Manufactured under an ISO 13485:2016 quality management system; aseptic fill and finish in Totowa, NJ, with every lot released against compendial test methods.
ISO 13485:2016 QMS
Documented, audited quality management system aligned with 21 CFR Part 820 (cGMP).
Ultrapure Type 1 Water
18.2 MΩ·cm water as the formulation base for low ionic and endotoxin background.
ISO Class 5 Fill & Finish
Aseptic filling in an ISO Class 5 (Class 100) environment in Totowa, NJ, right after the final 0.04 µm polish.
Micro-Batch Precision
Small, tightly controlled batches for consistent composition and pH from lot to lot.
Endotoxin — USP <85> BET
Released at < 0.05 EU/mL.
Particulate — USP <788> Method 2
Subvisible particles ≥10 µm and ≥25 µm within compendial limits.
Osmolality — USP <785>
Measured per lot and reported on the CoA.
Documentation / CoA
Appearance, pH 7.4 (± 0.04), 14-day sterility, endotoxin and osmolality per lot.
How DCP-DMEMH-Q2X compares
Same DMEM chemistry, different purity class. Conventional columns describe typical single-pass 0.22 µm-filtered catalog media.
| Parameter | DCP-DMEMH-Q2X (FluxMPS™) | Conventional DMEM High Glucose + HEPES, 1X (0.22 µm filtered) | Conventional 2X DMEM (0.22 µm filtered) |
|---|---|---|---|
| Formulation | 2X DMEM High Glucose + 50 mM HEPES; free of L-Glutamine | 1X DMEM, ready to use | Double-strength, for semisolid culture |
| Final filtration pore size | 0.04 µm | 0.22 µm | 0.22 µm |
| Number of filtration stages | 4 | 1 | 1 |
| Mycoplasma barrier filtration | check_circle | cancel | cancel |
| Endotoxin specification | < 0.05 EU/mL | Often not specified at this level | Often not specified at this level |
| USP <788> particulate compliance | check_circle | cancel | cancel |
| Ultrapure Type 1 water (18.2 MΩ·cm) | check_circle | Varies | Varies |
| ISO 13485:2016 manufacturing | check_circle | Varies | Varies |
| Microfluidic channel compatibility | check_circle | cancel | cancel |
| Custom formulation | check_circle | cancel | cancel |
Frequently asked questions
Common questions about using DCP-DMEMH-Q2X for semisolid, 3D, microfluidic and conventional culture.
Supporting literature
Peer-reviewed background on DMEM, HEPES buffering, semisolid and collagen-gel culture, Phenol Red, organ-on-a-chip culture and mycoplasma control.
- Dulbecco R, Freeman G. Plaque production by the polyoma virus. Virology. 1959;8(3):396–397. doi:10.1016/0042-6822(59)90043-1
- Eagle H. Nutrition needs of mammalian cells in tissue culture. Science. 1955;122(3168):501–504. doi:10.1126/science.122.3168.501
- Graham FL, Smiley J, Russell WC, Nairn R. Characteristics of a human cell line transformed by DNA from human adenovirus type 5. J Gen Virol. 1977;36(1):59–72. doi:10.1099/0022-1317-36-1-59
- Borowicz S, Van Scoyk M, Avasarala S, et al. The soft agar colony formation assay. J Vis Exp. 2014;(92):e51998. doi:10.3791/51998
- Bell E, Ivarsson B, Merrill C. Production of a tissue-like structure by contraction of collagen lattices by human fibroblasts of different proliferative potential in vitro. Proc Natl Acad Sci USA. 1979;76(3):1274–1278. doi:10.1073/pnas.76.3.1274
- Good NE, Winget GD, Winter W, Connolly TN, Izawa S, Singh RMM. Hydrogen ion buffers for biological research. Biochemistry. 1966;5(2):467–477. doi:10.1021/bi00866a011
- Berthois Y, Katzenellenbogen JA, Katzenellenbogen BS. Phenol red in tissue culture media is a weak estrogen: implications concerning the study of estrogen-responsive cells in culture. Proc Natl Acad Sci USA. 1986;83(8):2496–2500. doi:10.1073/pnas.83.8.2496
- Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32(8):760–772. doi:10.1038/nbt.2989
- Low LA, Mummery C, Berridge BR, Austin CP, Tagle DA. Organs-on-chips: into the next decade. Nat Rev Drug Discov. 2021;20(5):345–361. doi:10.1038/s41573-020-0079-3
- Leung CM, de Haan P, Ronaldson-Bouchard K, et al. A guide to the organ-on-a-chip. Nat Rev Methods Primers. 2022;2:33. doi:10.1038/s43586-022-00118-6
- Drexler HG, Uphoff CC. Mycoplasma contamination of cell cultures: incidence, sources, effects, detection, elimination, prevention. Cytotechnology. 2002;39(2):75–90. doi:10.1023/A:1022913015916
- Halldorsson S, Lucumi E, Gómez-Sjöberg R, Fleming RMT. Advantages and challenges of microfluidic cell culture in polydimethylsiloxane devices. Biosens Bioelectron. 2015;63:218–231. doi:10.1016/j.bios.2014.07.029