FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM), High Glucose w/o Sodium Pyruvate, Sodium Bicarbonate: 1X Liquid

FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM), High Glucose w/o Sodium Pyruvate, Sodium Bicarbonate: 1X Liquid

FluxMPS™ DCP-DMEM-PB1X is a Microfluidics Suitable, quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) High Glucose (4.5 g/L) DMEM formulated without sodium pyruvate and sodium bicarbonate, engineered for organ-on-a-chip (OoC), microphysiological systems (MPS), and energy-demanding cell models. A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration. Formulation: [+] High Glucose (4500 mg/L), [+] L-Glutamine, [+] Phenol Red, [+] Calcium, [+] Magnesium | [-] Sodium Pyruvate, [-] Sodium Bicarbonate.

• High Glucose (4.5 g/L) — supports energy-demanding cell types: primary neurons, cardiomyocytes, cancer cell lines
• Quadruple-stage filtration train — 0.1 µm Prefiltration I → 0.04 µm Final filtration I → 0.1 µm Prefiltration II → 0.04 µm Final filtration II, reaching a 0.04 µm final pore size
• Endotoxin release specification < 0.05 EU/mL (LAL, USP <85>), tested per manufacturing batch
• Formulation: [+] High Glucose (4500 mg/L), [+] L-Glutamine (584 mg/L), [+] Phenol Red, [+] Calcium, [+] Magnesium | [-] Sodium Pyruvate, [-] Sodium Bicarbonate
• Ultrapure Type 1 water (18.2 MΩ·cm) production input; manufactured under an ISO 13485:2016 quality management system
• CO₂-independent formulation — no sodium bicarbonate present; supplement with HEPES or another organic buffer for pH control
• Custom pH, salts, glucose concentration, and nutrient adjustments available on request

Engineered where standard media fails

Conventional 0.22 µm–filtered DMEM passes mycoplasma-scale organisms and subvisible particulates that accumulate in microfluidic channels and interfere with optical and electrical sensing. FluxMPS™ High Glucose is processed through a finer, repeated filtration train while delivering 4.5 g/L glucose for energy-demanding cell types and complex tissue models.

Quadruple-stage filtration system

Four sequential filtration stages reach a final 0.04 µm polish — two dedicated prefilter-plus-final-filter pairs in series, well beyond the single 0.22 µm pass typical of conventional media.

1. 1

   0.1 µm Prefiltration I

   Removes large particulate, cell debris, and protein aggregates; protects the first 0.04 µm cartridge from early fouling.

2. 2

   0.04 µm Final filtration I

   Retains sub-micron particulates and microaggregates that pass a 0.22 µm filter.

3. 3

   0.1 µm Prefiltration II

   A second dedicated prefilter, protecting the second 0.04 µm cartridge.

4. 4

   0.04 µm Final filtration II — Polish

   Ultimate polishing filter prior to fill & finish under validated ISO Class 5 conditions.

Designed for next-generation cell models

FluxMPS™ DCP-DMEM-PB1X supports platforms from single-channel microfluidic chips to multi-organ body-on-a-chip systems. High glucose (4.5 g/L) makes it particularly suited to energy-intensive and aerobically active cell types.

Automated Bioreactors & Robotics

An optional 0.01 µm (10 nm) ultra nano-filtered MPS Grade variant of this formulation is available on request for automated bioreactor perfusion and robotic liquid handlers — see the Grade note above for how this differs from the standard Microfluidics Suitable product.

• Total Particulate Exclusion: 10 nm filtration removes nanoparticulate aggregates
• Valve & Sensor Protection: Reduces micro-fouling risk to solenoid valves and optical sensors
• Extended Perfusion Stability: Consistent high-glucose delivery over weeks-long culture

Inquiry Required: Contact support@diagnocine.com for the 0.01 µm MPS Grade variant.

Analytical release specifications

Every lot released against the full specification matrix below. CoA: support@diagnocine.com.

Full composition (mg/L)

DMEM High Glucose is a modification of Basal Medium Eagle (BME) with 4× BME amino acid and vitamin concentrations, plus glycine, serine, and ferric nitrate. 31 ingredients verified per lot with CAS numbers for raw-material traceability.

Manufacturing & compliance

Every FluxMPS™ product is manufactured and released under a multi-layer quality system spanning raw materials, in-process controls, and final-product testing.

How DCP-DMEM-PB1X compares

FluxMPS™ DCP-DMEM-PB1X vs. conventional 0.22 µm–filtered DMEM High Glucose formulations.

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

Frequently asked questions

Common questions about FluxMPS™ DCP-DMEM-PB1X DMEM High Glucose.

Supporting literature

Key peer-reviewed publications supporting Microfluidics Suitable, ultra-filtered DMEM High Glucose in organ-on-a-chip and metabolic research.

1. Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
2. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
3. Warburg O. On the origin of cancer cells. Science. 1956;123:309–314. doi:10.1126/science.123.3191.309
4. Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x
5. Katt ME, et al. In vitro tumor models: advantages, disadvantages, variables, and selecting the right platform. Front Bioeng Biotechnol. 2016;4:12. doi:10.3389/fbioe.2016.00012
6. Jang KJ, et al. Human kidney proximal tubule-on-a-chip for drug transport and nephrotoxicity assessment. Integr Biol. 2013;5:1119–1129. doi:10.1039/c3ib40049b
7. Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588–3598. doi:10.1039/c3lc50217a
8. Sung JH, et al. Microfabricated mammalian organ systems and their integration into models of whole animals and humans. Lab Chip. 2013;13:1201–1212. doi:10.1039/c3lc41017j