Mueller Hinton Agar/W Penicillin G
Catalog No: DCP-MHAPPG
Features:
pH: 7.3±0.1
Type of Specimen: clinical samples (Pure cultures isolated from urine, stool, blood, etc)
Concentration: 10 µg/mL
Size: 100 mm
Storage: Keep away from light at 4°C
Shipping Condition: Blue ice
Appearance: Light amber colored clear to slightly opalescent
Sterile
Shelf Life: 2 weeks
*All agar plates are manufactured with ultrapure water, deionized and demineralized to minimize ionic and organic contaminants. The premium ISO certified agar solution is sterilized by a full cycle of autoclaving. Each 100mm plate has 20mL of agar medium in premium plastic dish
Description
Mueller Hinton Agar (MHA) agar plates with 10 µg/mL penicillin G is designed for the cultivation of most non-fastidious bacterial pathogens and for standardized antimicrobial susceptibility testing. Originally formulated as a clear agar for the cultivation of Neisseria species, MHA is now the reference medium for determining sulfonamide resistance in gonococci and other organisms, and is widely used for the diffusion of antimicrobial agents using the Kirby-Bauer disc diffusion method.
MHA demonstrates excellent batch-to-batch reproducibility for susceptibility testing, with low levels of sulfonamide, trimethoprim, and tetracycline inhibitors. The medium supports the growth of most non-fastidious bacteria and can be supplemented with 5% sheep blood or hemoglobin for testing fastidious organisms like Streptococcus pneumoniae and Haemophilus influenzae.
The addition of 10 µg/mL penicillin G renders the medium selective by inhibiting the growth of penicillin-sensitive bacteria, allowing for the isolation and testing of penicillin-resistant strains or gram-negative organisms from mixed samples.
Mueller Hinton Agar is not suitable for disc diffusion assays with slow-growing organisms, anaerobes, or capnophiles, as prolonged incubation can degrade antibiotics and yield imprecise results. It remains the recommended medium for routine antimicrobial diffusion testing using paper discs in clinical, research, and quality control laboratories
* This product is manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision). All final packaging, quality assurance, and testing are done at the DiagnoCine R&D and Quality Testing Center. All specific customization requests and assembly were accomplished at DiagnoCine Precision in Totowa, New Jersey, USA
