Flamma® 675 Maleimide

Flamma® Fluors 675 Maleimide is a cutting-edge near-infrared (NIR) fluorescent dye engineered for advanced bioimaging applications. This thiol-reactive fluorophore, derived from a benzindocyanine structure, offers exceptional stability in fluorescence signaling, making it an invaluable tool for researchers in life sciences.

Key features:
1. Excitation/Emission maxima: 675/691 nm
2. High extinction coefficient: ≥ 200,000 cm?¹M?¹
3. Maleimide reactive group for specific thiol labeling
4. Blue solid appearance
5. Molecular weight: 1067.23 g/mol
6. Soluble in DMF and DMSO

Applications:
1. Protein labeling (antibodies, peptides, proteins)
2. Ligand labeling for receptor studies
3. Amplification substrates in signal amplification techniques
4. In vitro imaging with high-sensitivity detection in fixed cells
5. Deep-tissue ex vivo and in vivo imaging
6. Potential use in super-resolution microscopy and single-molecule detection
7. Fluorescence lifetime imaging (FLIM) studies

Advantages:
1. Exceptional stability in fluorescence signaling
2. Optimal NIR spectrum operation, minimizing autofluorescence and allowing deep tissue penetration
3. Spectral similarity to popular NIR dyes (Alexa 680, Cy5.5, IRDye 680LT, DyLight 680, CF680)
4. Efficient excitation using a 633 nm laser line
5. Highly selective labeling of thiol groups, particularly cysteine residues
6. Formation of stable thioether linkages
7. Excellent performance in complex biological environments
8. Potential for multi-color imaging experiments
9. Reduced phototoxicity due to longer excitation wavelengths

Flamma® Fluors 675 Maleimide operates in the optimal NIR spectrum, with excitation at 675 nm and emission at 691 nm. This spectral range minimizes autofluorescence from biological samples, reduces light scattering, and allows for deep tissue penetration, making it ideal for in vivo and ex vivo imaging studies. The dye's high extinction coefficient (≥ 200,000 cm?¹M?¹) ensures brilliant fluorescence intensity, enabling the visualization of low-abundance biomolecules with exceptional clarity.

The maleimide reactive group enables highly specific and efficient labeling of thiol groups in biomolecules, particularly cysteine residues, via a 1,4-addition pathway. This selectivity results in minimal off-target labeling, producing cleaner and more accurate imaging results. The formed thioether linkages are exceptionally stable, providing long-lasting fluorescence signals ideal for extended imaging sessions. However, researchers should note that under strongly basic conditions, the dye may also react with primary amines.

Engineered to perform in complex biological environments, Flamma® Fluors 675 Maleimide exhibits excellent solubility in DMF and DMSO. Its spectral similarity to widely-used NIR dyes allows for seamless integration into existing imaging protocols and systems, making it a versatile choice for various advanced bioimaging techniques.

The NIR properties of Flamma® 675 Maleimide make it particularly valuable for in vivo imaging applications, enabling deeper penetration and reduced scattering in biological tissues. This characteristic is especially beneficial for studying complex biological systems, such as small animal models. Additionally, the dye's potential compatibility with fluorescence lifetime imaging (FLIM) techniques opens up possibilities for quantitative functional imaging studies.

For optimal results and longevity, store Flamma® Fluors 675 Maleimide at -20°C and protect it from light. With its superior brightness, excellent photostability, and high specificity, this dye empowers researchers to achieve unprecedented clarity and precision in their cellular and molecular imaging studies, contributing significantly to our understanding of complex biological processes at the molecular level.
 

Specifications

• Fluorophore: Flamma® Fluors 675
• Reactive group: Maleimide
• Excitation/Emission Max.(nm): 675/691 
• Spectrally similar dyes: Alexa680, DyLight680, Cy5.5, IRDye680LT, CF680
• Extinction coefficient: ≥ 200,000 cm-1M-1
• CF280: 0.18
• Appearance: Blue Solid
• Molecular Weight: 1067.23 g/mol   
• Solubility: DMF, DMSO
• Storage conditions: -20 ℃, protect from light 

 

Quick link (Cat.#)	FSD series	EXmax (nm)	EMmax (nm)	Spectrally similar dyes
CWM1001	Flamma® 496 Maleimide	496	520	Alexa488, FAM
CWM1058	Flamma® 552 Maleimide	550	565	Alexa555, DyLight549, Cy3, ATTO550, CF555
KWM1415	Flamma® 581 Maleimide	581	596	Alexa594, DyLight594
KWM1042	Flamma® 648 Maleimide	648	663	Alexa647, DyLight650, Cy5, ATTO 647N, CF647
PWM1415	Flamma® 675 Maleimide	675	691	Alexa680, DyLight680, Cy5.5, IRDye680LT, CF680
PWM1215	Flamma® 749 Maleimide	749	774	Alexa750, DyLight755, Cy7, IRDye750
PWM1515	Flamma® 774 Maleimide	774	806	Cy7.5, DyLight800, IRDye800

Background

• Covering the full spectral range from UV to NIR
• Equipped with a variety of reactive groups: NHS and Sulfo-NHS ester, Vinylsulfone, Maleimide, Click chemistry, isothiocyanate, hydrazide and hydrophobic substances.
• High quantum yields and photostability
• High purity and compatible with most of biomolecules

• High Sensitivity: Capable of detecting low-abundance biomolecules in fixed cells.
• NIR Emission: Allows for deep tissue penetration in biological samples.
• Stability: Provides consistent and reliable fluorescence signals.
• Versatility: Compatible with a wide range of biomolecules and imaging techniques.