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Flamma® 648 Hydrazide

Product#: PWH1215

$1,100.80

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1 mg
5 mg
25 mg

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Flamma® 648 Hydrazide

Cat. No. List below

Description

Flamma® Fluors 648 Hydrazide is a state-of-the-art far-red fluorescent dye engineered for exceptional performance in advanced bioimaging applications. This reactive fluorophore, derived from a cyanine structure, offers remarkable stability and brightness, making it an invaluable tool for researchers and imaging professionals.

Key features:
1. Excitation/Emission maxima: : 648/663 nm
2. High extinction coefficient: ≥ 220,000 cm?¹M?¹
3. Low CF280: 0.03
4. Hydrazide reactive group for specific aldehyde and ketone labeling
5. Blue solid appearance
6. Molecular weight: 698.89 g/mol
7. Soluble in DMF and DMSO
8. Spectrally similar to Alexa 647, DyLight 650, and Cy5

Applications:
1. Labeling of polysaccharides and glycoproteins
2. Visualization of free reducing sugars on biomolecules
3. Deep-tissue and whole-body imaging
4. 3D cell culture imaging (spheroids and organoids)
5. Live-cell imaging studies
6. Potential use in fluorescence-guided surgery
7. Multi-color imaging experiments

Advantages:
1. Generates stable fluorescence signals in bioimaging
2. Excellent optical properties when excited with 593 or 633 nm laser lines
3. Forms stable imine linkages through reductive amination reactions
4. Enables labeling of biomolecules after oxidation of primary and secondary alcohols
5. Offers compatibility with common fluorescence instrumentation
6. Provides a bright far-red fluorescence signal with minimal background
7. Enhanced tissue penetration and reduced light scattering
8. Minimal interference with protein absorbance measurements
9. Reduced phototoxicity and autofluorescence in biological samples
10. Suitable for long-term imaging due to photostability

Specifications

Fluorophore: Flamma® Fluors 648
Reactive group: Hydrazide
Excitation/Emission Max.(nm): 648/663
Spectrally similar dyes: Alexa647, DyLight650, Cy5
Extinction coefficient: ≥ 220,000 cm-1M-1
CF280:0.03
Appearance: Blue Solid
Molecular Weight: 698.89 g/mol
Solubility: DMF, DMSO
Storage conditions: -20 ℃, protect from light

Quick link (Cat.#)	FSD series	EXmax (nm)	EMmax (nm)	Spectrally similar dyes
CWH1001	Flamma® 496 Hydrazide	496	520	Alexa488, FITC, Cy2
PWH1122	Flamma® 552 Hydrazide	550	564	Alexa555, DyLight549, Cy3, ATTO550
KWH1415	Flamma® 581 Hydrazide	578	593	Alexa594, DyLight594
PWH1215	Flamma® 648 Hydrazide	648	663	Alexa647, DyLight650, Cy5
PWH1515	Flamma® 675 Hydrazide	675	691	Alexa680, DyLight680, Cy5.5, IRDye680LT, CF680
PWH1301	Flamma® 749 Hydrazide	749	774	Alexa750, DyLight755, Cy7.5, IRDye750
PWH1603	Flamma® 774 Hydrazide	774	800	Cy7.5, DyLight800, IRDye800

Background

Flamma® Fluors

BioActs offers a broad range of Flamma® Fluors dyes equipped with variety of reactive and functional groups, which can cover the full spectral range from UV to NIR with their excellent fluorescence performance. Characteristic features of these superior dyes are strong absorption, high fluorescence quantum yield and high photostability. Flamma® dyes maintain good fluorescence activity and stability after conjugation to biomolecules and allow the detection of low-abundance biological structures with great sensitivity. The dyes are compatible with optical conditions of most of fluorescent equipment and are ideal for any applications in biological studies.

Covering the full spectral range from UV to NIR
Equipped with a variety of reactive groups: NHS and Sulfo-NHS ester, Vinylsulfone, Maleimide, Click chemistry, isothiocyanate, hydrazide and hydrophobic substances.
High quantum yields and photostability
High purity and compatible with most of biomolecules

Why Choose Flamma® Fluors 648 Hydrazide?

Superior Brightness: High extinction coefficient ensures vivid fluorescence.
Excellent Stability: Generates stable fluorescence signals for reliable imaging.
Versatility: Compatible with various biomolecules and imaging setups.
Ease of Use: Simple conjugation process with clear guidelines.

Flamma® Fluors Hydrazide

Flamma^® Fluors hydrazide dyes can label aldehyde and ketone through reductive amination reaction to form an imine linkage. The main labeling target for hydrazides are free reducing sugars on biomolecules, and prior to conjugation, primary and secondary alcohols on polysaccharide and glycoprotein are usually oxidized to aldehyde and ketone. Fluorescent modification of aldehyde or carbonyl groups in carbohydrates is also frequently utilized for their analysis by HPLC, capillary electrophoresis and other methods. Hydrazide dyes can also label biomolecules, which introduced aldehyde by genetical or chemical modification.

Figure 1. Absorption (upper) and emission (bottom) spectra overlap of Flamma^® Fluors

Figure 2. Immunofluorescence imaging and in situ hybridization imaging

Figure 3. Fluorescence images of Flamma^® 749 (upper) and Flamma^®774 (bottom) carboxylic acid injected mouse model

Citation & Reference

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