Easy iMatrix-511 silk
Easy iMatrix-511 silk
| Cat. No.: | FNK-892024 |
| Size: | 100 mL |
| Ingredients: | Recombinant human laminin-511 E8 fragment, recombinant human serum albumin, phosphate buffered saline |
| Concentration: | 1.6 μg/mL of laminin-511E8 |
| Storage: | At 2-15°C in a dark place |
| Integrin Binding Affinity: | KD of laminin-511E8 and integrin α6β1 is 10 nM or less |
| Expiry date: | Labeled on the box |
| Use: | This product can be used as a cell culture substrate for iPS/ES and other cells |
Description
iMatrix-511 is a culture medium that can be used for various types of cells, and enables feeder-free culture of pluripotent stem cells (ES / iPS cells) and single-cell passage. This product "Easy iMatrix-511" can be coated on the culture vessel as it is without dilution.
The lineup includes Easy iMatrix-511 derived from CHO-S cells and Easy iMatrix-511 silk derived from silk moth cocoons.
* Recombinant Human Serum Albumin is added to stabilize iMatrix-511 and enhance its activity.
Background of Laminin and Laminin fragment
Laminin is one of the extra cellular matrix (ECM)s located in the basement membrane of animals, and play an important role for cell adhesion and proliferation.Laminin is composed of three parts ; alpha, beta, and gamma - chain and 15 types of laminin is already known. Among them, Laminin composed by Alpha5-chain, Beta1-chain, and Gamma1-chain, is called "Laminin-511".
Laminin-511 is recognized by alpha6beta1 integrin. Cell adhision, extension and proliferation is caused by many cell signalings through intergrin.Laminin-511 E8 is the fragment of Laminin-511 obtained by enzyme digestion. Although this is fragment, it has binding ability to alpha6beta1 integrin.
Features
- Ready to use
- Works in various cells - especially good for iPS / ES cells
- Reduce the risk of contamination by xeno-components in your culture system.
- High cell viability and proliferation rate.
Advantage of using Laminin-511E8 fragment in ES/iPS cell culture
With conventional culture methods, cells die if ES/iPS cells are dispersed into single cells. Therefore, maintaining cell colony is necessary to a certain extent when passaging.
Using Laminin-511 E8 fragment as cell culture substrate enables to maintain and culture even a single ES/iPS.
- The period of time required for learning cell culture methods is reduced.
- The extension efficiency of cell culture is drastically enhanced.
Procedure
- Add 1.5 mL / well of Easy iMatrix-511 to the plate.
- Incubate the plate at 37℃ for a hour at RT or 4℃ for overnight.
- After (2), discard solution and start culture shortly. (DO NOT DRY!)
Caution For iPS cells, recommended cell density is 2 - 3 x 103 cells/cm2. However, culture condition must be optimized.
References
- Ido, H., Nakamura, A., Kobayashi, R., Ito, S., Li, S., Futaki, S., Sekiguchi, K. The requirement of the glutamic acid residue at the third position from the carboxyl termini of the laminin gamma chains in integrin binding by laminins. J Biol Chem . 2007 Apr 13; 282 (15): 11144-11154.
- Taniguchi, Y., Ido, H., Sanzen, N., Hayashi, M., Sato-Nishiuchi, R., Futaki, S., Sekiguchi1, K. The C-terminal Region of Laminin Chains Modulates theIntegrin Binding Affinities of Laminins. J Biol Chem . 2009 Mar 20; 284 (12): 7820-7831.
- Miyazaki, T., Futaki, S., Suemori, H., Taniguchi, Y., Yamada, M., Kawasaki, M., Hayashi, M., Kumagai, H., Nakatsuji, N., Sekiguchi, K., Kawase E. Laminin E8 fragments support efficient adhesion and expansion of dissociated human pluripotent stem cells. Nat Commun . 2012 Dec 4; 3, 1236. Doi: 10.1038 / ncomms2231
iMatrix™ Cell Culture Substrates
| Product | Name | Product ID | Description | Usage | Size | Link |
 |
iMatrix-221 | FNK-892061 | Recombinant Human Laminin-211 E8 Fragments |
Cardiomyocytes | 2 × 175 μg |  |
| FNK-892062 | 6 × 175 μg | |
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 |
iMatrix-411 | FNK-892041 | Recombinant Human Laminin-411 E8 Fragments |
Endothelial cells | 2 × 175 μg | |
| FNK-892042 | 6 × 175 μg | |
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 |
iMatrix-511 | FNK-892011 | Recombinant Human Laminin-511 E8 Fragments |
Pluripotent Stem Cells | 2 × 175 μg | |
| FNK-892012 | 6 × 175 μg | |
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 |
iMatrix-511 Silk | FNK-892021 | Recombinant Human Laminin-511 E8 Fragments, expressed in Silkworm |
Pluripotent Stem Cells | 6 × 175 μg | |
