Easy iMatrix-511

Product#: 892018
$120.00
Availability:
Ships in 1-2 weeks

Easy iMatrix-511

 

Cat No: 892018
Size: 100 mL
Ingredients: Recombinant human laminin-511 E8 fragment, recombinant human serum albumin, phosphate buffered saline
Concentration: 1.6 μg/mL of laminin-511E8
Storage: At 2-15°C in a dark place
Integrin Binding Affinity: KD of laminin-511E8 and integrin α6β1 is 10 nM or less
Expiry date: Labeled on the box
Use: This product can be used as a cell culture substrate for iPS/ES and other cells


Description

iMatrix-511 is a culture medium that can be used for various types of cells, and enables feeder-free culture of pluripotent stem cells (ES / iPS cells) and single-cell passage. This product "Easy iMatrix-511" can be coated on the culture vessel as it is without dilution.
The lineup includes Easy iMatrix-511 derived from CHO-S cells and Easy iMatrix-511 silk derived from silk moth cocoons.
* Recombinant Human Serum Albumin is added to stabilize iMatrix-511 and enhance its activity.

Background of Laminin and Laminin fragment
 
Laminin is one of the extra cellular matrix (ECM)s located in the basement membrane of animals, and play an important role for cell adhesion and proliferation.Laminin is composed of three parts ; alpha, beta, and gamma - chain and 15 types of laminin is already known. Among them, Laminin composed by Alpha5-chain, Beta1-chain, and Gamma1-chain, is called "Laminin-511".

Laminin-511 is recognized by alpha6beta1 integrin. Cell adhision, extension and proliferation is caused by many cell signalings through intergrin.Laminin-511 E8 is the fragment of Laminin-511 obtained by enzyme digestion. Although this is fragment, it has binding ability to alpha6beta1 integrin.

892011_fig3.jpg 


Features
 

  • Ready to use
  • Works in various cells - especially good for iPS / ES cells
  • Reduce the risk of contamination by xeno-components in your culture system.
  • High cell viability and proliferation rate.
 Note: This product contains recombinant human serum albumin for stabilization and improvement of activity.


Advantage of using Laminin-511E8 fragment in ES/iPS cell culture
 
892018_fig1.png

With conventional culture methods, cells die if ES/iPS cells are dispersed into single cells. Therefore, maintaining cell colony is necessary to a certain extent when passaging.
892018_fig2.png
 

Using Laminin-511 E8 fragment as cell culture substrate enables to maintain and culture even a single ES/iPS.

  • The period of time required for learning cell culture methods is reduced.
  • The extension efficiency of cell culture is drastically enhanced.

Procedure
 
In the case of 6 well culture plate ... (9.6 cm2/well)
  1. Add 1.5 mL / well of Easy iMatrix-511 to the plate.
  2. Incubate the plate at 37℃ for a hour at RT or 4℃ for overnight.
  3. After (2), discard solution and start culture shortly. (DO NOT DRY!)


Caution: For iPS cells, recommended cell density is 2 - 3 x 103 cells/cm2.  However, culture condition must be optimized.


References

  1. Ido, H., Nakamura, A., Kobayashi, R., Ito, S., Li, S., Futaki, S., Sekiguchi, K. The requirement of the glutamic acid residue at the third position from the carboxyl termini of the laminin gamma chains in integrin binding by laminins. J Biol Chem . 2007 Apr 13; 282 (15): 11144-11154.
  2. Taniguchi, Y., Ido, H., Sanzen, N., Hayashi, M., Sato-Nishiuchi, R., Futaki, S., Sekiguchi1, K. The C-terminal Region of Laminin Chains Modulates theIntegrin Binding Affinities of Laminins. J Biol Chem . 2009 Mar 20; 284 (12): 7820-7831.
  3. Miyazaki, T., Futaki, S., Suemori, H., Taniguchi, Y., Yamada, M., Kawasaki, M., Hayashi, M., Kumagai, H., Nakatsuji, N., Sekiguchi, K., Kawase E. Laminin E8 fragments support efficient adhesion and expansion of dissociated human pluripotent stem cells. Nat Commun . 2012 Dec 4; 3, 1236. Doi: 10.1038 / ncomms2231


iMatrix™ Cell Culture Substrates

 
Product Name Product ID Description Usage Size Link
imatrix221_F0.png iMatrix-221 892061 Recombinant Human Laminin-211
E8 Fragments
Cardiomyocytes 2 × 175 μg Go_Button
892062 6 × 175 μg Go_Button
iMatrix411_F1.png iMatrix-411 892041 Recombinant Human Laminin-411
E8 Fragments
Endothelial cells 2 × 175 μg Go_Button
892042 6 × 175 μg Go_Button
iMatrix511_F1.png iMatrix-511 892011 Recombinant Human Laminin-511
E8 Fragments
Pluripotent Stem Cells 2 × 175 μg Go_Button
892012 6 × 175 μg Go_Button
iMatrix_511_Silk_F1.png iMatrix-511 Silk 892021 Recombinant Human Laminin-511
E8 Fragments, expressed in Silkworm
Pluripotent Stem Cells 6 × 175 μg Go_Button


 

Matrixome.pngNippi.png

 

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