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ECOS™ Competent Cells Description

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ECOS™ Competent Cells Description

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The Fastest Transforming Cells Worldwide - 1 minute Transformation

Fast transforming cells - exclusive technology
1 minute transformation

* With “ECOS™” standing for “E. Coli One-Step”, and its reverse “SOCE” standing for “SOC Eliminated”, the name itself speaks for the advantages.

* 1 minute, 1 step high-efficiency transformation and no SOC recovery step required, truly a design to save your time.

Competent Cell Types	ECOS™ Competent Cells (Diagnocine)	Traditional Chemically Competent Cells	Electro-Competent Cells
Time	1-6 minutes	1.5 ~ 2 hours	1.5 ~ 2.5 hours
Protocol	• One step • No incubation on ice after heat shock • No tube change • No recovery step	• Multiple steps • Requires incubation on ice after heat shock • Require tube change • Require recovery step	• Multiple steps • Requires incubation on ice after heat shock • Require tube change • Require recovery step
Authentic Representation	High	Require recovery step	Require recovery step
Transformation Efficiency	10⁷- 10¹⁰ cfu/1, pUC19	10⁶- 10⁹ cfu/μg pUC19	- 10¹⁰ cfu/μg pUC19
Cost	Low	High	High

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NAME	Cat NO.	STRAIN	EFFICIENCY	Application	SPECIFICATION	Order
ECOS^TM101	YBB-FYE607	DH5a	>1 x 108	Routine cloning	10 vials (100 μl/ vial)
					80 vials (100 μl/ vial)
ECOS^TMX	YBB-FYE610	DH5a	>5 x 109	Large plasmid Phage Display Difficult cloning	10 vials (100 μl/ vial)
					80 vials (100 μl/ vial)
ECOS^TMBLUE	YBB-FYE107	XL1-Blue	>5 x 107	Blue & White screen	10/80 vials (100 μl/ vial)
	YBB-FYE108		>2 x 108
	YBB-FYE109		>5 x 108
ECOS^TM21	YBB-FYE207	BL21(DE3)	>2 x 107	Protein expression	10 vials (100 μl/ vial)
					40 vials (100 μl/ vial)
ECOS^TM9-5	YBB-FYE707	JM109	>5 x 107	Fast growth	10/80 vials (100 μl/ vial)
	YBB-FYE709		>5 x 108
ECOS^TM 10B	YBB-FYE508	DH10B	>1 x 108	Large plasmid cDNAlibrary	10 vials (100 μl/ vial)
					/80 vials (100 μl/ vial)
ECOS^TM2163	YBB-FYE807	GM2163	>5 x 107	DAN Unmethylation	10/ vials (100 μl/ vial)
					80 vials (100 μl/ vial)
ECOS^TMSONIC	YBB-HYE007-A01	BL21(DE3) Derived	>1 x 108	Routine cloning & Protein expression	5 vials (100 μl/ vial)
ECOS^TMSONIC pLysS	YBB-HYE007-A02	BL21(DE3) pLysS Derived	>1 x 108	Toxic protein & membrane protein expression	20 vials (100 μl/ vial)

more 9-5 Competent Cells ECOS™ [JM109]

9-5 Competent Cells ECOS™ [JM109]	Cat.No.	Efficiency	Control Plasmid (pUC19)	size	vials/pack	Order
	YBB-JM109-FYE707-10VL	Efficiency >5 x 10⁷ cfu/μg	5 μl (10-4 μg/μl)	100 μl/vial	10 vials
	YBB-JM109-FYE707-80VL	Efficiency >5 x 10⁷ cfu/μg	5 μl (10-4 μg/μl)	100 μl/vial	80 vials
	YBB-JM109-FYE709-10VL	Efficiency >5 x 10⁸ cfu/μg	5 μl (10-4 μg/μl)	100 μl/vial	10 vials
	YBB-JM109-FYE709-80VL	Efficiency >5 x 10⁸ cfu/μg	5 μl (10-4 μg/μl)	100 μl/vial	80 vials

Transformation Protocol

Special Tips

Patented Technology

Yeastern Biotech Co., Ltd. owns the patent of ECOS™ technology (protocol) exclusively in USA, UK, Taiwan, and France (Patent No. US 6,864,088; GB 2383582; I 229696; FR 2832727, etc.). Under the protection of this patent, Yeastern Biotech is the only company that has the right to use the protocol with the claims that transformation with our ECOS™ chemically competent cells can be finished within one minute and SOC is not needed. If anyone intends to sell competent cells that use ECOS™ protocol or its similar version, please contact our headquarters in Taiwan for licensing.

Quality Control

Each lot of competent cells has to pass three quality control tests before shipping:

1. Efficiency test: each batch of ECOS™ should meet the claimed transformation efficiency at the time of production using Protocol 4 (Page 3-3) and supercoiled pUC19 DNA.

2. Contamination test: competent cells were plated directly on ampicillin plates without being transformed. To pass the test, no colonies should be seen after overnight incubation.

3. A-complementation test: this test is performed for all ECOS™ competent cells except for ECOS™ 21(DE3) and ECOS™ 2163. To pass the test, the ratio of white colonies over the total colonies should be less than 3%

=Q & A=

ECOS competent cells Test

I.   Q: How many operating methods can operate ECOS?
A: Four methods: One to Six minutes of operating.
(The fastest to the highest efficiency)

II.   Q: How long is the transformation of ECOS competent cells by using the fast operation method?
A: One minute

III.   Q: What is the highest efficiency achieved by ECOS™ X (DH5α) high-efficiency operation?
A: 5*10^9 cfu/μg

IV.   Q: What is the advantage of ECOS competent cells?
A:
a.   One Step; Time-saving
b.   Easy to operate
c.   No SOC recovery
d.   No tube change

V.   Q: What is the shelf life of ECOS competent cells?
A: One to One and a half years

VI.   Q: During the thawing step of operating ECOS competent cells, how far does it need to be thawed?
A: One half to Two-thirds thawed

VII.   Q: If customers choose Kanamycin as antibiotics resistant gene screening, what suggestions should we inform them?
A: Use SOC for Recovery

VIII.   Q: How should we set the temperature and time when operating the step of Heat Shock?
A: 42°C and Forty-five seconds

IX.   Q: What is the achievable efficiency ranking of the four operation modes on the ECOS card?
A: Three>One>Four>Two

X.   Q: When cultured to produce plasmids, which ECOS can be completed in as fast as 4 hours?
A: ECOS™ SONIC™

XI.   Q: When the colony is cultivated, we usually set different time according to the strain, also the time range is usually from four to sixteen hours, however, what is the fixed temperature should be set?
A: 37°C

XII.   Q: What is the usual time and temperature for colony culture of ECOS™ 101 Competent Cells [DH5α]?
A: 37°C and Twelve to Sixteen hours

XIII.   Q: When Plasmid fragment>6kb, which kinds of ECOS competent cell is used ideal?
A: ECOS™X, ECOS™101, ECOS 10B

XIV.   Q: How much will the efficiency be reduced if ECOSTM competent cells are thawed, dispensed, and refrozen repeatedly?
A: If ECOSTM competent cells are thawed, dispensed in aliquots, and refrozen within 3 min, the transformation efficiency will be 20~50% less than first time use.

XV.   Q: Which the humidity of LB plate is good for the efficiency? Dry or Wet?
A: Dry

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