ECOS™ 101 Competent Cells [DH5α]

Cat.No.:   YBB-FYE607-80VL


Description

General Cloning: DH5α is the most popular E. coli strain for everyday cloning applications. It supports blue/white screening for easy selection of recombinant DNA with X-Gal. In addition, DH5α carries recA1 mutation that eliminates homologous recombination ensuring insert stability. It also carries endA1 mutations that greatly improve the quality of plasmid DNA and yield prepared from mini-prep. It is useful for the transformation of large plasmids and two-hybrid systems (up to 40 kb).

Features
Suitable for cloning with large plasmid and cDNA library construction, and also allows for blue-white colony screening.

Applications

• Cloning and subcloning
• Scale-up application
• Blue/white screening

Technical / Specifications

   Store at   -70°C ~  -80°C

   Genotype
   endA1 recA1 relA1 deoR gyrA96 nupG hsdR17(rk^–, mk⁺) supE44 thi-1 Δ(lacZYA-argF)U169 Φ80 lacZΔM15 λ^– F^–

  Size:  80 vials 



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   # YBB-FYE607-10VL (10 preps)

Efficiency >1 x 108 cfu/μg
100 μl/vial	10 vials
Control Plasmid (pUC19)	5 μl (10-4 μg/μl)

   # YBB-FYE607-80VL (80 preps)

Efficiency >1 x 108 cfu/μg
100 μl/vial	80 vials
Control Plasmid (pUC19)	5 μl (10-4 μg/μl)