ECOS™ 2163 Competent Cells [GM2163]

Cat. No.:    YBB-FYE807-80VL


Description

GM2163 is an E. coli K12 strain, which is deficient in both dam and dcm genes, is suitable for the propagation of plasmid or cloned DNA to be cut with Dam or Dcm-sensitive restriction enzymes.
This strain is not recommended as a host for primary cloning/ligation since the dam mutation can result in higher mutation rates and a reduction in the transformation efficiency.


Features

• Chloramphenicol resistant
• Not suitable for blue/white screening
• Not recommended as a host for primary cloning/ligation

Applications

• Propagation of plasmid-free of Dam and Dcm methylations

Technical / Specifications

   Store:  at   -70°C ~  -80°C

   Genotype: F^–ara^–14 leuB6 fhuA31 lacY1 tsx78 glnV44 galK2 galT22 mcrA dcm^–6 hisG4 rfbD1 rpsL136 dam13::Tn9 xylA5 mtl^–1 thi-1 mcrB1 hsdR2

   Size:  80 vials 

    


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# YBB-FYE807-10VL (10 preps)

Efficiency >1 x 107 cfu/μg
100 μl/vial	10 vials
Control Plasmid (pUC19)	5 μl (10-4 μg/μl)

# YBB-FYE807-80VL (80 preps)

Efficiency >1 x 107 cfu/μg
100 μl/vial	80 vials
Control Plasmid (pUC19)	5 μl (10-4 μg/μl)