ECOS™ 9-5 Competent Cells [JM109]

Cat. No.:   YBB-JM109-FYE707-10VL 

Description

Fast Growth: JM109, a K strain bacterium, carries mutations in recA and endA, leading to minimal recombination and improved quality of isolated plasmid DNA. In addition, the cells carry the F’ episome which allows blue/white screening for recombinant DNA. Thus, it has been a popular strain for routine subcloning. The cells can also be utilized for single-stranded DNA rescue when M13 or phagemid systems are used simultaneously. The JM109 strain is sensitive to all common antibiotics.

This product can become a powerful cloning tool when it is used in combination with Yeastern’s rapid ligation kit (#FYC003-100R). The ligation can be finished within 5 minutes and followed by 1-minute transformation. The fast growth rate of JM109 allows colonies to show up within 8 hours. Try this strain when you are in urgent.

Features

• Rapid growing strain
• Carries F’ episome

Applications

• Preparation of ssDNA
• Construction of gene library
• Subcloning

Technical / Specifications

   Store at   -70°C ~  -80°C

   Genotype
   e14^–(McrA^–) recA1 endA1 gyrA96 thi-1 hsdR17(rK^– mK⁺) supE44 relA1 D(lac–proAB) [F’ traD36 proAB lacIqZDM15]

  Size:  10 vials 
      

       
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ECOS™ 9-5 Competent Cells [JM109]
	Cat. No.(FYE707):
	YBB-JM109-FYE707-10VL      YBB-JM109-FYE707-80VL
	Cat. No.(FYE709):
	YBB-JM109-FYE709-10VL      YBB-JM109-FYE709-80VL

Specifications

9-5 Competent Cells ECOS™ [JM109]	Cat.No.	Efficiency	Control Plasmid (pUC19)	size	vials/pack	link
	YBB-JM109-FYE707-10VL	Efficiency >5 x 107 cfu/μg	5 μl (10-4 μg/μl)	100 μl/vial	10 vials
	YBB-JM109-FYE707-80VL	Efficiency >5 x 107 cfu/μg	5 μl (10-4 μg/μl)	100 μl/vial	80 vials
	YBB-JM109-FYE709-10VL	Efficiency >5 x 108 cfu/μg	5 μl (10-4 μg/μl)	100 μl/vial	10 vials
	YBB-JM109-FYE709-80VL	Efficiency >5 x 108 cfu/μg	5 μl (10-4 μg/μl)	100 μl/vial	80 vials