ECOS™ Sonic™ Competent Cells [BL21(DE3) Derived]

Cat. No.:  YBB-HYE007-Sonic-20VL


Description

We have taken it upon ourselves to continue the production of Acella™ cells previously from Edge Biosystems Inc., which we have now renamed as ECOS™ Sonic™

Sonic™ Competent Cells allow you to clone and express in one novel strain. Also available with the pLysS plasmid, the Sonic™ strain is a BL21-derivative that features complete deletions of the endA and recA genes, eliminating degradation and recombination of DNA.

For optimal results, recommended for use in combination with Yeastern’s proprietary T&A™ expression vector, which will combine transformation, cloning and protein expression into one single step.


 
Features

• Fast: Only 4-6hrs culture time required to isolates plasmid DNA
• Stable: Lack of ompT and Ion proteases to stabilize synthesized protein; deletion of endA and recA eliminates DNA degradation and recombination
• High efficiency and protein yield: 1 x 10⁸ cfu/ug
• Simple to use: clone and express with the same strain, no more sub-cloning required!

Applications

• protein expression/ toxic protein expression
• preservation of the PCR product
• preservation of the expression plasmid

Specifications

  Genotype:   F^– ompT hsds_B (r_B^–m_B^–) gal dcm (DE3) ΔendA ΔrecA
 
  Store at   -70°C ~  -80°C

  Size:  20 vials 

   


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# YBB-HYE007-Sonic-5VL (5 preps)

Efficiency >1 x 108 cfu/μg
100 μl/vial	5 vials
Control Plasmid (pUC19)	5 μl (10-4 μg/μl)

 

#  YBB-HYE007-Sonic-20VL (20 preps)

Efficiency >1 x 108 cfu/μg
100 μl/vial	20 vials
Control Plasmid (pUC19)	5 μl (10-4 μg/μl)