ECOS™ 21 Competent Cells [BL21(DE3)]

Cat. No.:     YBB-FYE207-10VL 


Description
This strain provides high levels of protein expression. This strain carries the lambda DE3 lysogen, which expresses T7 RNA polymerase from the lacUV5 promoter by isopropyl-1-thio-ß-D-galactopyranoside (IPTG) induction. The transformation efficiency of BL-21 is usually low, so the 6 minute/ heat shock cold plating protocol is recommended if high efficiency is desired.

 
Features

• lack of Lon and Omp proteases allows high-level protein expression and accumulation
• Easy gene induction

Applications

• IPTG induced gene expression in E. coli
• Ideal for high-level expression of non-toxic recombinant protein expression using T7-based expression vectors
• Subcloning

Specifications

   Genotype:   F^– ompT gal dcm lon hsdSB(rB^– mB^–) λ(DE3 [lacI lacUV5-T7 gene 1 ind1 sam7 nin5])

   Store at   -70°C ~  -80°C

   Size:  10 vials 


       

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# YBB-FYE207-40VL (40 preps)

Efficiency >2 x 107 cfu/μg
100 μl/vial	40 vials
Control Plasmid (pUC19)	5 μl (10-4 μg/μl)

     
# YBB-FYE207-10VL (10 preps)

Efficiency >2 x 107 cfu/μg
100 μl/vial	10 vials
Control Plasmid (pUC19)	5 μl (10-4 μg/μl)