FluxMPS™ Dulbecco’s Modified Eagle Medium/ Ham's F-12 Nutrient Mixture (DMEM/F-12) (1:1) with 15mM HEPES w/o L-Glutamine, Sodium Bicarbonate: 1X Liquid

FluxMPS™ Dulbecco’s Modified Eagle Medium/ Ham's F-12 Nutrient Mixture (DMEM/F-12) (1:1) with 15mM HEPES w/o L-Glutamine, Sodium Bicarbonate: 1X Liquid

FluxMPS™ Dulbecco’s Modified Eagle Medium/ Ham's F-12 Nutrient Mixture (DMEM/F-12) (1:1) with 15mM HEPES w/o L-Glutamine, Sodium Bicarbonate: 1X Liquid is a Microfluidics Suitable, quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) 1X liquid cell culture medium engineered for organ-on-a-chip (OoC), tissue-on-a-chip (ToC), and microphysiological system (MPS) applications. A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration.

• Quadruple-stage nano-filtration: 0.1 µm (Prefiltration I & II) + 0.04 µm (Final filtration I & II), reaching a 0.04 µm final cut-off
• Endotoxin release specification: < 0.05 EU/mL (USP <85> BET, tested per manufacturing batch)
• DMEM/F-12 (1:1) base with 15 mM HEPES buffering; formulated without L-glutamine and without sodium bicarbonate — pH 7.4
• Prepared with Ultrapure Type 1 water (18.2 MΩ·cm)
• Aseptic fill & finish in an ISO Class 5 environment under an ISO 13485:2016 quality management system
• Mycoplasma risk mitigated via 0.1 µm mycoplasma-retentive filtration (not tested per lot)
• Phenol red present (8.630 mg/L) for visual pH monitoring — not compatible with phenol red-free assay platforms (e.g., Agilent Seahorse XF)
• Custom formulations available — pH, glucose, salts, HEPES, and nutrient composition on request

Engineered where standard media fails

Conventional 0.22 µm-filtered media carry mycoplasma-sized particulates (0.2–0.3 µm), subvisible debris, and variable endotoxin load that accumulate inside microchannels — corrupting biosensor readings and shortening device lifetimes. FluxMPS™ addresses these failure modes through a validated multi-stage filtration process.

Quadruple-stage filtration system

FluxMPS™ Dulbecco’s Modified Eagle Medium/ Ham's F-12 Nutrient Mixture (DMEM/F-12) (1:1) with 15mM HEPES w/o L-Glutamine, Sodium Bicarbonate: 1X Liquid is processed through a four-stage serial filtration sequence (0.1 µm → 0.04 µm → 0.1 µm → 0.04 µm) reaching a 0.04 µm final cut-off — substantially reducing particulate load relative to single-pass 0.22 µm filtration used in conventional media.

1. 1

   0.1 µm Prefiltration I

   Removes large particulates and aggregates; protects the first 0.04 µm final filter cartridge and extends filter life.

2. 2

   0.04 µm Final filtration I

   First 0.04 µm pass; retains sub-micron particulates and material in the mycoplasma size range (0.2–0.3 µm) that a 0.22 µm filter does not.

3. 3

   0.1 µm Prefiltration II

   Second, dedicated prefilter stage protecting the second 0.04 µm cartridge from residual particulate load.

4. 4

   0.04 µm Final filtration II — Polish

   Ultimate polishing filtration prior to aseptic fill & finish in a validated ISO Class 5 laminar-flow workstation.

Designed for next-generation cell culture platforms

FluxMPS™ Dulbecco’s Modified Eagle Medium/ Ham's F-12 Nutrient Mixture (DMEM/F-12) (1:1) with 15mM HEPES w/o L-Glutamine, Sodium Bicarbonate: 1X Liquid is suited to organ-on-a-chip, metabolic research, live-cell imaging, and primary cell models where particulate contamination and endotoxin variation must be minimized.

Automated Bioreactors & Robotics

An optional 0.01 µm (10 nm) ultra nano-filtered MPS Grade variant is available for automated bioreactor and robotic perfusion systems requiring the ultimate particulate exclusion.

• Total Particulate Exclusion: 0.01 µm filtration for nanoscale valve and sensor protection
• Valve & Sensor Protection: reduces particulate-induced blockage risk in precision fluidic systems
• Extended Perfusion Stability: supports flow rate consistency across multi-week automated runs

Inquiry Required: Contact support@diagnocine.com to request the 0.01 µm MPS Grade variant.

Lot-release quality parameters

Every production lot of FluxMPS™ Dulbecco’s Modified Eagle Medium/ Ham's F-12 Nutrient Mixture (DMEM/F-12) (1:1) with 15mM HEPES w/o L-Glutamine, Sodium Bicarbonate: 1X Liquid undergoes the complete quality-release battery below before shipment.

Full composition (mg/L)

This formulation contains 51 defined components across four categories — Inorganic Salts, Amino Acids, Vitamins, and Others — reproduced in full below with CAS numbers where available. Custom compositions available on request.

ISO 13485:2016 manufacturing & compliance

Manufactured under an ISO 13485:2016 quality management system with final packaging, testing, and customization at Diagnocine, Totowa, New Jersey, USA.

How DCP-DM12H-QBT1X (FluxMPS™) compares

Side-by-side comparison against conventional 0.22 µm-filtered alternatives of the same base formulation.

Comparison figures from published supplier specifications, accessed 2 September 2026. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

Frequently asked questions

Common questions about FluxMPS™ Dulbecco’s Modified Eagle Medium/ Ham's F-12 Nutrient Mixture (DMEM/F-12) (1:1) with 15mM HEPES w/o L-Glutamine, Sodium Bicarbonate: 1X Liquid and Microfluidics Suitable cell culture media.

Supporting literature

Peer-reviewed publications supporting ultra-filtered, Microfluidics Suitable media and microfluidic cell culture applications.

1. Huh D et al. (2010). Reconstituting organ-level lung functions on a chip. Science, 328(5986), 1662–1668. doi:10.1126/science.1188302
2. Bhatia SN & Ingber DE (2014). Microfluidic organs-on-chips. Nature Biotechnology, 32(8), 760–772. doi:10.1038/nbt.2989
3. Eagle H (1959). Amino acid metabolism in mammalian cell cultures. Science, 130(3373), 432–437. doi:10.1126/science.130.3373.432
4. Bhattacharya S et al. (2018). Challenges in maintaining cell viability during microfluidic experiments. Electrophoresis, 39(7), 997–1006. doi:10.1002/elps.201700375
5. Zhang YS et al. (2017). Multisensor-integrated organs-on-chips platform for automated in situ monitoring. PNAS, 114(12), E2293–E2302. doi:10.1073/pnas.1612906114
6. Vernetti L et al. (2017). Functional coupling of human microphysiology systems. Scientific Reports, 7, 42296. doi:10.1038/srep42296
7. Esch EW et al. (2015). Organs-on-chips at the frontiers of drug discovery. Nature Reviews Drug Discovery, 14(4), 248–260. doi:10.1038/nrd4539
8. Zheng F et al. (2021). Organ-on-a-chip systems: microengineering to biomimic living systems. Small, 17(7), 2004175. doi:10.1002/smll.202004175