- Home
- FluxMPS™ Dulbecco’s Modified Eagle Medium/ Ham's F-12 Nutrient Mixture (DMEM/F-12) (1:1) with Trace Elements w/o L-Glutamine, Phenol Red: 2X Liquid
FluxMPS™ Dulbecco’s Modified Eagle Medium/ Ham's F-12 Nutrient Mixture (DMEM/F-12) (1:1) with Trace Elements w/o L-Glutamine, Phenol Red: 2X Liquid
FluxMPS™ Dulbecco's Modified Eagle Medium/Ham's F-12 Nutrient Mixture (DMEM/F-12) (1:1) with Trace Elements w/o L-Glutamine, Phenol Red: 2X Liquid
Double-strength (2X) DMEM/F-12 (1:1) with trace elements — for defined and serum-reduced media development, stem-cell, organoid, epithelial and primary-cell culture, ultra-filtered through a quadruple-stage 0.1 µm / 0.04 µm filtration system. With a sodium bicarbonate buffer and no Phenol Red, it is approximately 5× cleaner than conventional 0.22 µm-filtered media by particulate count.
- 2X concentrate: dilute to 1X or mix with an equal volume of a compatible solution, cell suspension or hydrogel
- Trace elements included: selenium, zinc, copper, iron, manganese, molybdenum, vanadium, nickel, silicon and tin salts
- Quadruple-stage filtration: 0.1 µm membrane filtered twice, 0.04 µm membrane filtered twice
- 0.04 µm nano-filtration final polish acts as a mycoplasma barrier
- Endotoxin < 0.05 EU/mL (USP <85> BET)
- Formulation: [+] 6.3 g/L Glucose, [+] Sodium Pyruvate, [+] Sodium Bicarbonate, [+] Trace Elements; [−] L-Glutamine, [−] Phenol Red, [−] HEPES
- Ultrapure Type 1 water (18.2 MΩ·cm); ISO Class 5 (Class 100) aseptic fill in Totowa, NJ
- Custom formulations available — pH, glucose, HEPES, salts, and nutrient composition on request
- D-Glucose6,302 mg/L
- L-GlutamineNot included
- Sodium Pyruvate220 mg/L
- BufferNaHCO₃ 2,400 mg/L
- Phenol RedNot included
- AppearancePale Yellow
- pH7.4 (± 0.04)
- Endotoxin< 0.05 EU/mL
- Filtration0.1 µm ×2 + 0.04 µm ×2
- Format2X liquid, 500 mL
Engineered where standard media fails
Conventional DMEM is sterile-filtered once at 0.22 µm. That pore size can pass mycoplasma and leaves subvisible particulates that settle in microchannels, foul membranes and hydrogels, and add noise to optical readouts.[11,12] FluxMPS™ DMEM keeps Dulbecco's formulation and removes that failure mode.
Microchannel-safe purity
A 0.04 µm final filter and USP <788> particulate control keep subvisible debris out of chips, tubing, membranes and matrices.
Total metabolic control
Defined 6,302 mg/L D-glucose, no L-glutamine and 220 mg/L sodium pyruvate in the 2X (half that at 1X).
Ultrapure-grade water
Made with Ultrapure Type 1 water (18.2 MΩ·cm) and released at < 0.05 EU/mL endotoxin by USP <85>.
Low background for imaging
Particle-depleted medium reduces scatter and debris artifacts in colony counting, confocal imaging and on-chip sensing, and the Phenol Red-free formulation removes color background.
Rich DMEM/F-12 nutrient base
57 components at double strength, including vitamin B12, linoleic acid, lipoic acid, putrescine, hypoxanthine and thymidine from Ham's F-12.
Customization on demand
pH, glucose, HEPES, salts, and nutrient composition on request — contact support@diagnocine.com.
Quadruple-stage filtration system
Every lot of DCP-DM12-QR2X is membrane filtered at 0.1 µm twice and 0.04 µm twice — a ready-to-dilute 2X DMEM polished to a 0.04 µm final pore size, a purity level single-pass 0.22 µm media cannot reach.
-
01
0.1 µmPre-filtration I
Removes large particulates and aggregates and extends the life of the downstream filters.
-
02
0.04 µmPre-filtration II
Retains fine particulates, bacteria and mycoplasma.
-
03
0.1 µmSterile-filtration I
Second-pass redundancy that catches anything shed or bypassed upstream.
-
04
0.04 µmSterile-filtration II — Final Polish
Final nano-polish immediately before ISO Class 5 aseptic fill.
Performance vs. conventional media
By particulate count, FluxMPS™ DMEM is approximately 5× cleaner than conventional 0.22 µm-filtered media, lowering clog risk in narrow channels and long perfusion runs.
© Diagnocine® — DCP-DM12-QR2X
Where FluxMPS™ DMEM fits
DMEM/F-12 (1:1) With Trace Elements combines Dulbecco’s Modified Eagle Medium and Ham’s F-12 Nutrient Mixture in an equal ratio.[1,2,3] It provides amino acids, vitamins, salts, glucose, and trace elements to support a broad range of mammalian cells in laboratory culture.
This nutrient-rich formulation is commonly used for in vitro epithelial, primary-cell, stem-cell, organoid, and other research cell-culture applications. It is also suitable for developing serum-reduced or defined research media when used with appropriate supplements.[4,5]
DMEM/F-12 (1:1) 2X With Trace Elements is a double-strength formulation designed to be diluted to 1X or combined with an equal volume of another compatible solution. The 2X format helps maintain the intended final 1X concentrations of basal nutrients and trace elements when adding large volumes of supplements, treatment solutions, cell suspensions, hydrogels, or other matrices.
It is useful for:
- Development of defined or serum-reduced research media.
- In vitro epithelial and primary-cell research.
- Research-use stem-cell and organoid culture.
- Nutrient-response and cellular-metabolism studies.
- Laboratory-scale semisolid, hydrogel, and 3D cell culture.
- Research-use tissue-engineering and organ-on-a-chip studies.
- Customized media preparation with added growth factors or supplements.
- Experimental workflows requiring concentrated basal medium.
Automated Bioreactors & Robotics
For automated bioreactors, liquid-handling robots and closed perfusion loops with fine valves and in-line sensors, an optional 10 nm (0.01 µm) ultra-filtered grade of this medium is available.
- Total Particulate Exclusion: 0.01 µm filtration removes virtually all particulates down to the nanometer scale.
- Valve & Sensor Protection: keeps micro-valves, pumps and optical or electrochemical sensors free of deposits.
- Extended Perfusion Stability: supports long, uninterrupted perfusion runs with fewer line changes.
Inquiry Required: the 0.01 µm grade is made on request — email support@diagnocine.com with your catalog number and volume.
Defined & Serum-Reduced Media
A rich, defined DMEM/F-12 base for building serum-reduced and customized research media with growth factors and other supplements.[4]
Complete Trace-Element Package
Selenium, zinc, copper, iron, manganese and other trace elements are already in the formulation, supporting low-serum and defined culture without a separate trace-element supplement.[3,4]
Stem-Cell & Organoid Culture
DMEM/F-12 is a common basal medium for stem-cell and organoid protocols; the 2X format leaves room for growth-factor cocktails and matrices.[5]
Epithelial & Primary Cells
Supports epithelial and other primary cells in defined or low-serum culture after dilution to 1X.[3,4]
Organ-on-a-Chip & MPS
Microchannel-safe medium for perfused organ-, tissue- and body-on-a-chip platforms.[8,9,10]
Microscopy & Optical Sensing
Fewer particles means cleaner fields for colony counting, confocal imaging and on-chip biosensors; no Phenol Red background.
Specifications & quality control
Release specifications for DCP-DM12-QR2X. Lot-specific values are reported on the Certificate of Analysis.
| Parameter | Specification |
|---|---|
| Formulation | [+] 6.3 g/L Glucose, [+] Sodium Pyruvate, [+] Sodium Bicarbonate, [+] Trace Elements; [−] L-Glutamine, [−] Phenol Red, [−] HEPES |
| Concentration | 2X (dilute 1:1 to 1X) |
| Appearance | Pale Yellow |
| pH | 7.4 (± 0.04) USP <791> |
| Osmolality | Measured per lot; reported on CoA USP <785> |
| D-Glucose | 6,302 mg/L (6.3 g/L; 3.15 g/L at 1X) |
| L-Glutamine | Not included |
| Sodium Pyruvate | 220 mg/L |
| Sodium Bicarbonate | 2,400 mg/L |
| Phenol Red | Not included |
| HEPES | Not included |
| Trace elements | Included (Se, Zn, Cu, Fe, Mn, Mo, V, Ni, Si, Sn) |
| Parameter | Specification |
|---|---|
| Endotoxin | < 0.05 EU/mL USP <85> BET |
| Sterility | No bacterial or fungal growth after 14 days of incubation USP <71> |
| Mycoplasma | 0.04 µm barrier filtration USP <63> equiv. |
| Particulate ≥10 µm | Meets limits USP <788> M2 |
| Particulate ≥25 µm | Meets limits USP <788> M2 |
| Water purity | Ultrapure Type 1, 18.2 MΩ·cm |
| Manufacturing std. | ISO 13485:2016 ISO |
| Fill environment | ISO Class 5 (Class 100) ISO |
| Parameter | Specification |
|---|---|
| Storage temperature | 2–8 °C, protected from light |
| Freeze-thaw | Do not freeze |
| Shelf life | 12 months; use before the expiry date on the product label |
| Shipping condition | Ice Pack |
| CO₂ requirement | CO₂-controlled incubator after dilution to 1X; sodium bicarbonate 2,400 mg/L in the 2X, 1,200 mg/L at 1X |
| Parameter | Specification |
|---|---|
| Raw material grade | High-purity, cell-culture-tested components |
| Traceability | Lot-traceable; CoA per lot |
| Manufacturing QMS | ISO 13485:2016 ISO |
| Regulatory alignment | 21 CFR Part 820 (cGMP) aligned |
| Production method | Micro-batch 2X liquid, quadruple-stage filtered; aseptic fill and finish in Totowa, NJ |
| Intended use | For Research Use Only (RUO) |
Full composition (mg/L)
DCP-DM12-QR2X is 2X DMEM/F-12 (1:1) with 6.3 g/L glucose, sodium pyruvate, sodium bicarbonate and trace elements. It does not contain L-glutamine, Phenol Red nor HEPES. Users are advised to review the literature for recommendations regarding medium supplementation and physiological growth requirements specific for different cell lines. All 57 components are listed below at 2X strength; values are per-lot release basis and are halved after 1:1 dilution.
| Component | CAS Number | mg/L |
|---|---|---|
| INORGANIC SALTS | ||
| Ammonium metavanadate | 7803-55-6 | 0.00116 |
| Ammonium molybdate tetrahydrate | 12054-85-2 | 0.01236 |
| Calcium chloride dihydrate | 10035-04-8 | 309.000 |
| Copper sulphate pentahydrate | 7758-99-8 | 0.0026 |
| Disodium hydrogen phosphate | 7558-79-4 | 142.040 |
| Ferric nitrate nonahydrate | 7782-61-8 | 0.100 |
| Ferrous sulphate heptahydrate | 7782-63-0 | 0.834 |
| Magnesium chloride hexahydrate | 7791-18-6 | 122.400 |
| Magnesium sulphate anhydrous | 7487-88-9 | 97.680 |
| Manganese sulphate | 7785-87-7 | 0.000302 |
| Nickel chloride | 7718-54-9 | 0.00024 |
| Potassium chloride | 7447-40-7 | 623.600 |
| Sodium bicarbonate | 144-55-8 | 2400.000 |
| Sodium chloride | 7647-14-5 | 13992.000 |
| Sodium dihydrogen phosphate | 7558-80-7 | 108.600 |
| Sodium metasilicate nonahydrate | 13517-24-3 | 0.0284 |
| Sodium selenite | 10102-18-8 | 0.01038 |
| Stannous chloride dihydrate | 10025-69-1 | 0.00022 |
| Zinc sulphate heptahydrate | 7446-20-0 | 0.864 |
| Component | CAS Number | mg/L |
|---|---|---|
| AMINO ACIDS | ||
| Glycine | 56-40-6 | 37.500 |
| L-Alanine | 56-41-7 | 8.900 |
| L-Arginine hydrochloride | 1119-34-2 | 295.000 |
| L-Asparagine monohydrate | 5794-13-8 | 15.000 |
| L-Aspartic acid | 56-84-8 | 13.300 |
| L-Cysteine hydrochloride monohydrate | 7048-04-6 | 35.120 |
| L-Cystine dihydrochloride | 30925-07-6 | 62.580 |
| L-Glutamic acid | 56-86-0 | 14.700 |
| L-Histidine hydrochloride monohydrate | 5934-29-2 | 62.960 |
| L-Isoleucine | 73-32-5 | 108.940 |
| L-Leucine | 61-90-5 | 118.100 |
| L-Lysine hydrochloride | 657-27-2 | 182.500 |
| L-Methionine | 63-68-3 | 34.480 |
| L-Phenylalanine | 63-91-2 | 70.960 |
| L-Proline | 147-85-3 | 34.500 |
| L-Serine | 56-45-1 | 52.500 |
| L-Threonine | 72-19-5 | 106.900 |
| L-Tryptophan | 73-22-3 | 18.040 |
| L-Tyrosine disodium salt | 69847-45-6 | 96.200 |
| L-Valine | 72-18-4 | 105.700 |
| Component | CAS Number | mg/L |
|---|---|---|
| VITAMINS | ||
| Choline chloride | 67-48-1 | 17.960 |
| D-Biotin | 58-85-5 | 0.0070 |
| D-Ca-Pantothenate | 137-08-6 | 4.480 |
| Folic acid | 59-30-3 | 5.320 |
| Niacinamide | 98-92-0 | 4.040 |
| Pyridoxal hydrochloride | 65-22-5 | 4.000 |
| Pyridoxine hydrochloride | 58-56-0 | 0.062 |
| Riboflavin | 83-88-5 | 0.438 |
| Thiamine hydrochloride | 67-03-8 | 4.340 |
| Vitamin B12 | 68-19-9 | 1.360 |
| myo-Inositol | 87-89-8 | 25.200 |
| OTHERS | ||
| D-Glucose | 50-99-7 | 6302.000 |
| DL-Thioctic acid | 1077-28-7 | 0.210 |
| Hypoxanthine sodium salt | 45738-97-4 | 4.800 |
| Linoleic acid | 60-33-3 | 0.084 |
| Putrescine hydrochloride | 333-93-7 | 0.162 |
| Sodium pyruvate | 113-24-6 | 220.000 |
| Thymidine | 50-89-5 | 0.730 |
Manufacturing & compliance
Manufactured under an ISO 13485:2016 quality management system; aseptic fill and finish in Totowa, NJ, with every lot released against compendial test methods.
ISO 13485:2016 QMS
Documented, audited quality management system aligned with 21 CFR Part 820 (cGMP).
Ultrapure Type 1 Water
18.2 MΩ·cm water as the formulation base for low ionic and endotoxin background.
ISO Class 5 Fill & Finish
Aseptic filling in an ISO Class 5 (Class 100) environment in Totowa, NJ, right after the final 0.04 µm polish.
Micro-Batch Precision
Small, tightly controlled batches for consistent composition and pH from lot to lot.
Endotoxin — USP <85> BET
Released at < 0.05 EU/mL.
Particulate — USP <788> Method 2
Subvisible particles ≥10 µm and ≥25 µm within compendial limits.
Osmolality — USP <785>
Measured per lot and reported on the CoA.
Documentation / CoA
Appearance, pH 7.4 (± 0.04), 14-day sterility, endotoxin and osmolality per lot.
How DCP-DM12-QR2X compares
Same DMEM chemistry, different purity class. Conventional columns describe typical single-pass 0.22 µm-filtered catalog media.
| Parameter | DCP-DM12-QR2X (FluxMPS™) | Conventional DMEM/F-12, 1X (0.22 µm filtered) | Conventional 2X medium (0.22 µm filtered) |
|---|---|---|---|
| Formulation | 2X DMEM/F-12 (1:1) with trace elements; free of L-Glutamine, phenol red and HEPES | 1X DMEM/F-12, ready to use | Double-strength, general purpose |
| Final filtration pore size | 0.04 µm | 0.22 µm | 0.22 µm |
| Number of filtration stages | 4 | 1 | 1 |
| Mycoplasma barrier filtration | check_circle | cancel | cancel |
| Endotoxin specification | < 0.05 EU/mL | Often not specified at this level | Often not specified at this level |
| USP <788> particulate compliance | check_circle | cancel | cancel |
| Ultrapure Type 1 water (18.2 MΩ·cm) | check_circle | Varies | Varies |
| ISO 13485:2016 manufacturing | check_circle | Varies | Varies |
| Microfluidic channel compatibility | check_circle | cancel | cancel |
| Custom formulation | check_circle | cancel | cancel |
Frequently asked questions
Common questions about using DCP-DM12-QR2X for semisolid, 3D, microfluidic and conventional culture.
Supporting literature
Peer-reviewed background on DMEM and Ham's F-12, serum-free and organoid culture, HEPES buffering, organ-on-a-chip culture and mycoplasma control.
- Dulbecco R, Freeman G. Plaque production by the polyoma virus. Virology. 1959;8(3):396–397. doi:10.1016/0042-6822(59)90043-1
- Eagle H. Nutrition needs of mammalian cells in tissue culture. Science. 1955;122(3168):501–504. doi:10.1126/science.122.3168.501
- Ham RG. Clonal growth of mammalian cells in a chemically defined, synthetic medium. Proc Natl Acad Sci USA. 1965;53(2):288–293. doi:10.1073/pnas.53.2.288
- Barnes D, Sato G. Methods for growth of cultured cells in serum-free medium. Anal Biochem. 1980;102(2):255–270. doi:10.1016/0003-2697(80)90151-7
- Sato T, Vries RG, Snippert HJ, et al. Single Lgr5 stem cells build crypt-villus structures in vitro without a mesenchymal niche. Nature. 2009;459(7244):262–265. doi:10.1038/nature07935
- Good NE, Winget GD, Winter W, Connolly TN, Izawa S, Singh RMM. Hydrogen ion buffers for biological research. Biochemistry. 1966;5(2):467–477. doi:10.1021/bi00866a011
- Berthois Y, Katzenellenbogen JA, Katzenellenbogen BS. Phenol red in tissue culture media is a weak estrogen: implications concerning the study of estrogen-responsive cells in culture. Proc Natl Acad Sci USA. 1986;83(8):2496–2500. doi:10.1073/pnas.83.8.2496
- Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32(8):760–772. doi:10.1038/nbt.2989
- Low LA, Mummery C, Berridge BR, Austin CP, Tagle DA. Organs-on-chips: into the next decade. Nat Rev Drug Discov. 2021;20(5):345–361. doi:10.1038/s41573-020-0079-3
- Leung CM, de Haan P, Ronaldson-Bouchard K, et al. A guide to the organ-on-a-chip. Nat Rev Methods Primers. 2022;2:33. doi:10.1038/s43586-022-00118-6
- Drexler HG, Uphoff CC. Mycoplasma contamination of cell cultures: incidence, sources, effects, detection, elimination, prevention. Cytotechnology. 2002;39(2):75–90. doi:10.1023/A:1022913015916
- Halldorsson S, Lucumi E, Gómez-Sjöberg R, Fleming RMT. Advantages and challenges of microfluidic cell culture in polydimethylsiloxane devices. Biosens Bioelectron. 2015;63:218–231. doi:10.1016/j.bios.2014.07.029