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FluxMPS™ Dulbecco’s Modified Eagle Medium/ Ham's F-12 Nutrient Mixture (DMEM/F-12) (1:1) w/o Sodium Bicarbonate: 2X Liquid

Product#: DCP-DM12-BT2X
$115.50
DCP-DM12-BT2X
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FluxMPS™ Dulbecco's Modified Eagle Medium/Ham's F-12 Nutrient Mixture (DMEM/F-12) (1:1) w/o Sodium Bicarbonate: 2X Liquid

Double-strength (2X) DMEM/F-12 (1:1) without added trace elements — for defined and serum-free media development, stem-cell, organoid, epithelial and primary-cell culture, ultra-filtered through a quadruple-stage 0.1 µm / 0.04 µm filtration system. With a bicarbonate-free base for user-defined buffering, it is approximately 5× cleaner than conventional 0.22 µm-filtered media by particulate count.

  • 2X concentrate: dilute to 1X or mix with an equal volume of a compatible solution, cell suspension or hydrogel
  • No added trace elements, so you control trace-element supplementation
  • Quadruple-stage filtration: 0.1 µm membrane filtered twice, 0.04 µm membrane filtered twice
  • 0.04 µm nano-filtration final polish acts as a mycoplasma barrier
  • Endotoxin < 0.05 EU/mL (USP <85> BET)
  • Formulation: [+] 6.3 g/L Glucose, [+] L-Glutamine, [+] Sodium Pyruvate, [+] Phenol Red; [−] Sodium Bicarbonate, [−] HEPES, [−] Trace Elements
  • Ultrapure Type 1 water (18.2 MΩ·cm); ISO Class 5 (Class 100) aseptic fill in Totowa, NJ
  • Custom formulations available — pH, glucose, HEPES, salts, and nutrient composition on request
DCP-DM12-BT2X|UNSPSC 41116155Cell culture media
Catalog No. DCP-DM12-BT2X · Size: 500 mL
Dulbecco's Modified Eagle Medium/Ham's F-12 Nutrient Mixture (DMEM/F-12) (1:1) w/o Sodium Bicarbonate: 2X Liquid
  • D-Glucose6,302 mg/L
  • L-Glutamine730 mg/L
  • Sodium Pyruvate220 mg/L
  • BufferNone (user-defined)
  • Phenol Red17.26 mg/L
  • AppearanceRed
  • pH7.4 (± 0.04)
  • Endotoxin< 0.05 EU/mL
  • Filtration0.1 µm ×2 + 0.04 µm ×2
  • Format2X liquid, 500 mL
ISO 13485:2016USP <85> <785> <788>RUO
Why FluxMPS™

Engineered where standard media fails

Conventional DMEM is sterile-filtered once at 0.22 µm. That pore size can pass mycoplasma and leaves subvisible particulates that settle in microchannels, foul membranes and hydrogels, and add noise to optical readouts.[11,12] FluxMPS™ DMEM keeps Dulbecco's formulation and removes that failure mode.

filter_alt

Microchannel-safe purity

A 0.04 µm final filter and USP <788> particulate control keep subvisible debris out of chips, tubing, membranes and matrices.

target

Total metabolic control

Defined 6,302 mg/L D-glucose, 730 mg/L L-glutamine and 220 mg/L sodium pyruvate in the 2X (half that at 1X).

water_drop

Ultrapure-grade water

Made with Ultrapure Type 1 water (18.2 MΩ·cm) and released at < 0.05 EU/mL endotoxin by USP <85>.

visibility

Low background for imaging

Particle-depleted medium reduces scatter and debris artifacts in colony counting, confocal imaging and on-chip sensing.

science

Rich DMEM/F-12 nutrient base

51 components at double strength, including vitamin B12, linoleic acid, lipoic acid, putrescine, hypoxanthine and thymidine from Ham's F-12.

tune

Customization on demand

pH, glucose, HEPES, salts, and nutrient composition on request — contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

Every lot of DCP-DM12-BT2X is membrane filtered at 0.1 µm twice and 0.04 µm twice — a ready-to-dilute 2X DMEM polished to a 0.04 µm final pore size, a purity level single-pass 0.22 µm media cannot reach.

  1. 01

    0.1 µmPre-filtration I

    Removes large particulates and aggregates and extends the life of the downstream filters.

  2. 02

    0.04 µmPre-filtration II

    Retains fine particulates, bacteria and mycoplasma.

  3. 03

    0.1 µmSterile-filtration I

    Second-pass redundancy that catches anything shed or bypassed upstream.

  4. 04

    0.04 µmSterile-filtration II — Final Polish

    Final nano-polish immediately before ISO Class 5 aseptic fill.

Performance vs. conventional media

By particulate count, FluxMPS™ DMEM is approximately 5× cleaner than conventional 0.22 µm-filtered media, lowering clog risk in narrow channels and long perfusion runs.

5×
cleaner by particulate count vs. 0.22 µm media
0.04
µm final filtration pore size
Sterility & mycoplasma assurance: no bacterial or fungal growth is observed after 14 days of incubation, as per USP specification (USP <71>), and the 0.04 µm stages provide USP <63>-equivalent mycoplasma assurance.[11]
FluxMPS™ Dulbecco's Modified Eagle Medium/Ham's F-12 Nutrient Mixture (DMEM/F-12) (1:1) w/o Sodium Bicarbonate: 2X Liquid (DCP-DM12-BT2X) Quadruple-stage filtration system diagram: 0.1 μm pre-filtration, 0.04 μm pre-filtration, 0.1 μm sterile filtration and 0.04 μm final polish for MPS-grade DMEM/F-12 (1:1) cell culture media used in stem-cell, organoid, organ-on-a-chip and microfluidic applications, Diagnocine
Figure 1. Quadruple-stage filtration: 0.1 µm pre-filtration, 0.04 µm pre-filtration, 0.1 µm sterile filtration and 0.04 µm final polish before ISO Class 5 fill.
© Diagnocine® — DCP-DM12-BT2X
Applications

Where FluxMPS™ DMEM fits

DMEM/F-12 (1:1) Without Trace Elements combines Dulbecco’s Modified Eagle Medium and Ham’s F-12 Nutrient Mixture while omitting added trace elements.[1,2,3] This specialized formulation allows researchers to control trace-element supplementation and is suitable for customized media development, nutrient-response studies, stem-cell culture, organoid culture, epithelial-cell research, and other defined cell-culture applications.[4,5]

DMEM/F-12 (1:1) 2X Without Trace Elements is a double-strength formulation designed to be diluted to 1X or mixed with an equal volume of another compatible solution. The 2X format maintains the intended final 1X concentration of basal nutrients after adding supplements, treatment solutions, cell suspensions, hydrogels, or other high-volume components.

It is useful for:

  • In vitro trace-element depletion and supplementation studies.
  • Laboratory development of defined or serum-free research media.
  • In vitro nutrient-response and cellular-metabolism research.
  • Research-use stem-cell and organoid culture.
  • In vitro epithelial and primary-cell research.
  • Laboratory-scale semisolid, hydrogel, and 3D cell-culture applications.
  • Research-use tissue-engineering and organ-on-a-chip studies.
  • Development of customized research media with controlled trace-element concentrations.

Automated Bioreactors & Robotics

Next-Generation System Uptime

For automated bioreactors, liquid-handling robots and closed perfusion loops with fine valves and in-line sensors, an optional 10 nm (0.01 µm) ultra-filtered grade of this medium is available.

  • Total Particulate Exclusion: 0.01 µm filtration removes virtually all particulates down to the nanometer scale.
  • Valve & Sensor Protection: keeps micro-valves, pumps and optical or electrochemical sensors free of deposits.
  • Extended Perfusion Stability: supports long, uninterrupted perfusion runs with fewer line changes.

Inquiry Required: the 0.01 µm grade is made on request — email support@diagnocine.com with your catalog number and volume.

Media Development

Defined & Serum-Free Media

A rich, defined DMEM/F-12 base for building serum-free and customized research media with your own supplements.[4]

Serum-freeDefined mediaCustom supplements
Trace Elements

Trace-Element Studies

No added trace elements, so you can titrate selenium, manganese and other trace elements in depletion and supplementation experiments.

DepletionSupplementationNutrient response
Stem Cell Biology

Stem-Cell & Organoid Culture

DMEM/F-12 is a common basal medium for stem-cell and organoid protocols; the 2X format leaves room for growth-factor cocktails and matrices.[5]

OrganoidsiPSC/ESCMatrigel/hydrogels
Cell Biology

Epithelial & Primary Cells

Supports epithelial and other primary cells in defined or low-serum culture after dilution to 1X.[3,4]

EpithelialPrimary cellsLow serum
Microfluidics

Organ-on-a-Chip & MPS

Microchannel-safe medium for perfused organ-, tissue- and body-on-a-chip platforms.[8,9,10]

OoCToCMPS
Live-Cell Imaging

Microscopy & Optical Sensing

Fewer particles means cleaner fields for colony counting, confocal imaging and on-chip biosensors.

ConfocalColony countingBiosensors
Technical Specifications

Specifications & quality control

Release specifications for DCP-DM12-BT2X. Lot-specific values are reported on the Certificate of Analysis.

Physical & Chemical Parameters
Parameter Specification
Formulation [+] 6.3 g/L Glucose, [+] L-Glutamine, [+] Sodium Pyruvate, [+] Phenol Red; [−] Sodium Bicarbonate, [−] HEPES, [−] Trace Elements
Concentration 2X (dilute 1:1 to 1X)
Appearance Red
pH 7.4 (± 0.04) USP <791>
Osmolality Measured per lot; reported on CoA USP <785>
D-Glucose 6,302 mg/L (6.3 g/L; 3.15 g/L at 1X)
L-Glutamine 730 mg/L
Sodium Pyruvate 220 mg/L
Sodium Bicarbonate Not included
Phenol Red 17.26 mg/L
HEPES Not included
Added trace elements Not included
Sterility, Purity & Safety Parameters
Parameter Specification
Endotoxin < 0.05 EU/mL USP <85> BET
Sterility No bacterial or fungal growth after 14 days of incubation USP <71>
Mycoplasma 0.04 µm barrier filtration USP <63> equiv.
Particulate ≥10 µm Meets limits USP <788> M2
Particulate ≥25 µm Meets limits USP <788> M2
Water purity Ultrapure Type 1, 18.2 MΩ·cm
Manufacturing std. ISO 13485:2016 ISO
Fill environment ISO Class 5 (Class 100) ISO
Storage, Handling & Logistics
Parameter Specification
Storage temperature 2–8 °C, protected from light
Freeze-thaw Do not freeze
Shelf life 12 months; use before the expiry date on the product label
Shipping condition Ice Pack
CO₂ requirement Depends on the buffer you add; supply sodium bicarbonate before use in a CO₂ incubator
Raw Materials & Regulatory Traceability
Parameter Specification
Raw material grade High-purity, cell-culture-tested components
Traceability Lot-traceable; CoA per lot
Manufacturing QMS ISO 13485:2016 ISO
Regulatory alignment 21 CFR Part 820 (cGMP) aligned
Production method Micro-batch 2X liquid, quadruple-stage filtered; aseptic fill and finish in Totowa, NJ
Intended use For Research Use Only (RUO)
Note: pH and sodium bicarbonate concentration of the prepared medium are critical factors affecting cell growth. This is also influenced by amount of medium and volume of culture vessel used (surface to volume ratio). For example, in large bottles, such as Roux bottles, pH tends to rise perceptibly as a significant volume of carbon dioxide is released. Therefore, optimal conditions of pH, sodium bicarbonate concentration and surface to volume ratio must be determined for each cell type. We recommend stringent monitoring of pH. If needed, pH can be adjusted by using sterile 1N HCl or 1N NaOH or by bubbling in carbon dioxide. If required, supplements can be added to the medium prior to or after filter sterilization observing sterility precautions.
Formulation

Full composition (mg/L)

DCP-DM12-BT2X is 2X DMEM/F-12 (1:1) with 6.3 g/L glucose, L-glutamine, sodium pyruvate and Phenol Red. It does not contain sodium bicarbonate, HEPES nor added trace elements. Users are advised to review the literature for recommendations regarding medium supplementation and physiological growth requirements specific for different cell lines. All 51 components are listed below at 2X strength; values are per-lot release basis and are halved after 1:1 dilution.

Component CAS Number mg/L
INORGANIC SALTS
Calcium chloride dihydrate 10035-04-8 309.000
Copper sulphate pentahydrate 7758-99-8 0.0026
Disodium hydrogen phosphate, anhydrous 7558-79-4 142.040
Ferric nitrate nonahydrate 7782-61-8 0.100
Ferrous sulphate heptahydrate 7782-63-0 0.834
Magnesium chloride anhydrous 7786-30-3 122.400
Magnesium sulphate anhydrous 7487-88-9 97.680
Potassium chloride 7447-40-7 623.600
Sodium chloride 7647-14-5 13992.000
Sodium dihydrogen phosphate anhydrous 7558-80-7 108.600
Zinc sulphate heptahydrate 7446-20-0 0.864
Component CAS Number mg/L
AMINO ACIDS
Glycine 56-40-6 37.500
L-Alanine 56-41-7 8.900
L-Arginine hydrochloride 1119-34-2 295.000
L-Asparagine monohydrate 5794-13-8 15.000
L-Aspartic acid 56-84-8 13.300
L-Cysteine hydrochloride monohydrate 7048-04-6 35.120
L-Cystine dihydrochloride 30925-07-6 62.580
L-Glutamic acid 56-86-0 14.700
L-Glutamine 56-85-9 730.000
L-Histidine hydrochloride monohydrate 5934-29-2 62.960
L-Isoleucine 73-32-5 108.940
L-Leucine 61-90-5 118.100
L-Lysine hydrochloride 657-27-2 182.500
L-Methionine 63-68-3 34.480
L-Phenylalanine 63-91-2 70.960
L-Proline 147-85-3 34.500
L-Serine 56-45-1 52.500
L-Threonine 72-19-5 106.900
L-Tryptophan 73-22-3 18.040
L-Tyrosine disodium salt 69847-45-6 96.200
L-Valine 72-18-4 105.700
Component CAS Number mg/L
VITAMINS
Choline chloride 67-48-1 17.960
D-Biotin 58-85-5 0.0070
D-Ca-Pantothenate 137-08-6 4.480
Folic acid 59-30-3 5.320
Niacinamide 98-92-0 4.040
Pyridoxal hydrochloride 65-22-5 4.000
Pyridoxine hydrochloride 58-56-0 0.062
Riboflavin 83-88-5 0.438
Thiamine hydrochloride 67-03-8 4.340
Vitamin B12 68-19-9 1.360
myo-Inositol 87-89-8 25.200
OTHERS
D-Glucose 50-99-7 6302.000
DL-Thioctic Acid 1077-28-7 0.210
Hypoxanthine sodium salt 45738-97-4 4.800
Linoleic acid 60-33-3 0.084
Phenol Red 34487-61-1 17.260
Putrescine hydrochloride 333-93-7 0.162
Sodium pyruvate 113-24-6 220.000
Thymidine 50-89-5 0.730
Customization: Custom formulations available — pH, glucose, HEPES, salts, and nutrient composition on request. Contact support@diagnocine.com.
Quality Assurance

Manufacturing & compliance

Manufactured under an ISO 13485:2016 quality management system; aseptic fill and finish in Totowa, NJ, with every lot released against compendial test methods.

verified

ISO 13485:2016 QMS

Documented, audited quality management system aligned with 21 CFR Part 820 (cGMP).

water_drop

Ultrapure Type 1 Water

18.2 MΩ·cm water as the formulation base for low ionic and endotoxin background.

biotech

ISO Class 5 Fill & Finish

Aseptic filling in an ISO Class 5 (Class 100) environment in Totowa, NJ, right after the final 0.04 µm polish.

assignment

Micro-Batch Precision

Small, tightly controlled batches for consistent composition and pH from lot to lot.

Endotoxin — USP <85> BET

Released at < 0.05 EU/mL.

Particulate — USP <788> Method 2

Subvisible particles ≥10 µm and ≥25 µm within compendial limits.

Osmolality — USP <785>

Measured per lot and reported on the CoA.

Documentation / CoA

Appearance, pH 7.4 (± 0.04), 14-day sterility, endotoxin and osmolality per lot.

A lot-specific Certificate of Analysis is available for every lot — request it at support@diagnocine.com.
Product Comparison

How DCP-DM12-BT2X compares

Same DMEM chemistry, different purity class. Conventional columns describe typical single-pass 0.22 µm-filtered catalog media.

Parameter DCP-DM12-BT2X (FluxMPS™) Conventional DMEM/F-12, 1X (0.22 µm filtered) Conventional 2X medium (0.22 µm filtered)
Formulation 2X DMEM/F-12 (1:1); free of sodium bicarbonate, HEPES and trace elements 1X DMEM/F-12, ready to use Double-strength, general purpose
Final filtration pore size 0.04 µm 0.22 µm 0.22 µm
Number of filtration stages 4 1 1
Mycoplasma barrier filtration check_circle cancel cancel
Endotoxin specification < 0.05 EU/mL Often not specified at this level Often not specified at this level
USP <788> particulate compliance check_circle cancel cancel
Ultrapure Type 1 water (18.2 MΩ·cm) check_circle Varies Varies
ISO 13485:2016 manufacturing check_circle Varies Varies
Microfluidic channel compatibility check_circle cancel cancel
Custom formulation check_circle cancel cancel
FAQ

Frequently asked questions

Common questions about using DCP-DM12-BT2X for semisolid, 3D, microfluidic and conventional culture.

Yes. DCP-DM12-BT2X is quadruple-stage filtered to a 0.04 µm final pore size, so it carries far fewer subvisible particles than 0.22 µm media — approximately 5× cleaner by particulate count. That lowers the risk of clogging microchannels, valves and membranes in organ-on-a-chip, hydrogel-based 3D and microfluidic perfusion systems.[8,9,10]
The medium is filtered four times: 0.1 µm twice and 0.04 µm twice. A single 0.22 µm pass can let mycoplasma and fine particulates through; the 0.04 µm stages act as a mycoplasma barrier and polish out sub-micron particles before ISO Class 5 aseptic fill.[11]
Leaving sodium bicarbonate out lets the solution or matrix you mix with the 2X carry the buffer, or lets you set your own bicarbonate level for your CO₂ tension. Our complete 2X formulation carries 2,400 mg/L sodium bicarbonate (1,200 mg/L at 1X); pH and sodium bicarbonate concentration are critical to cell growth, so monitor pH closely. HEPES is not included, so the medium relies on its bicarbonate buffer in a CO₂ incubator; choose the 30 mM HEPES version if you need extra buffering outside the incubator. Added trace elements are left out so you can set them yourself — useful for trace-element depletion and supplementation studies and for building defined or serum-free media with controlled trace-element levels.[4] Note that the base formulation still carries the copper, iron and zinc salts listed in the composition table. Users are advised to review the literature for supplementation and physiological growth requirements specific to each cell type.[1,2]
It depends on the buffer you add. With sodium bicarbonate, use a CO₂-controlled incubator; with a non-volatile buffer such as HEPES, CO₂ is not needed for pH control.
Mix 2X DMEM with an equal volume of a compatible solution or matrix — supplements, treatment solutions, cell suspensions or hydrogels — so glucose (3.15 g/L), amino acids, vitamins and salts end up at their 1X concentration.Serum, antibiotics and other supplements can be added before or after filter sterilization, observing sterility precautions.
The endotoxin specification is < 0.05 EU/mL, tested by the USP <85> bacterial endotoxins test (BET) and reported on the lot Certificate of Analysis. The medium is made with Ultrapure Type 1 water (18.2 MΩ·cm).
Yes. Each lot ships with a CoA covering appearance (red), pH 7.4 (± 0.04), sterility (no bacterial or fungal growth after 14 days of incubation per USP), endotoxin and osmolality results. Request copies at support@diagnocine.com.
Scientific References

Supporting literature

Peer-reviewed background on DMEM and Ham's F-12, serum-free and organoid culture, HEPES buffering, organ-on-a-chip culture and mycoplasma control.

  1. Dulbecco R, Freeman G. Plaque production by the polyoma virus. Virology. 1959;8(3):396–397. doi:10.1016/0042-6822(59)90043-1
  2. Eagle H. Nutrition needs of mammalian cells in tissue culture. Science. 1955;122(3168):501–504. doi:10.1126/science.122.3168.501
  3. Ham RG. Clonal growth of mammalian cells in a chemically defined, synthetic medium. Proc Natl Acad Sci USA. 1965;53(2):288–293. doi:10.1073/pnas.53.2.288
  4. Barnes D, Sato G. Methods for growth of cultured cells in serum-free medium. Anal Biochem. 1980;102(2):255–270. doi:10.1016/0003-2697(80)90151-7
  5. Sato T, Vries RG, Snippert HJ, et al. Single Lgr5 stem cells build crypt-villus structures in vitro without a mesenchymal niche. Nature. 2009;459(7244):262–265. doi:10.1038/nature07935
  6. Good NE, Winget GD, Winter W, Connolly TN, Izawa S, Singh RMM. Hydrogen ion buffers for biological research. Biochemistry. 1966;5(2):467–477. doi:10.1021/bi00866a011
  7. Berthois Y, Katzenellenbogen JA, Katzenellenbogen BS. Phenol red in tissue culture media is a weak estrogen: implications concerning the study of estrogen-responsive cells in culture. Proc Natl Acad Sci USA. 1986;83(8):2496–2500. doi:10.1073/pnas.83.8.2496
  8. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32(8):760–772. doi:10.1038/nbt.2989
  9. Low LA, Mummery C, Berridge BR, Austin CP, Tagle DA. Organs-on-chips: into the next decade. Nat Rev Drug Discov. 2021;20(5):345–361. doi:10.1038/s41573-020-0079-3
  10. Leung CM, de Haan P, Ronaldson-Bouchard K, et al. A guide to the organ-on-a-chip. Nat Rev Methods Primers. 2022;2:33. doi:10.1038/s43586-022-00118-6
  11. Drexler HG, Uphoff CC. Mycoplasma contamination of cell cultures: incidence, sources, effects, detection, elimination, prevention. Cytotechnology. 2002;39(2):75–90. doi:10.1023/A:1022913015916
  12. Halldorsson S, Lucumi E, Gómez-Sjöberg R, Fleming RMT. Advantages and challenges of microfluidic cell culture in polydimethylsiloxane devices. Biosens Bioelectron. 2015;63:218–231. doi:10.1016/j.bios.2014.07.029

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