Columbia Agar Base Plates/W Chloramphenicol
Catalog No: DCP-CABPCP
pH: 7.3 ± 0.2
Uses: Standard method base for enumeration and detection of bacteria in food and environmental samples, and clinical speciments
Size: 100mm
Concentration: 50 µg/mL
Storage: Keep away from light at 4°C
Shipping Condition: Blue ice
Apeearance: light amber, clear to slightly opalescent firm gel. Adding 5% sheep (or horse/rabbit) blood, the medium becomes opaque red
Sterile
Shelf Life: 4 Weeks
*All agar plates are manufactured with ultrapure water, deionized and demineralized to minimize ionic and organic contaminants. The premium ISO certified agar solution is sterilized by a full cycle of autoclaving. Each 100mm plate has 20mL of agar medium in premium plastic dish
Description:
Columbia Agar Base with chloramphenicol is a nutritionally rich, non-blood agar medium in which standard Columbia agar base is supplemented with the broad-spectrum antibiotic chloramphenicol to create a selective environment that suppresses most bacteria while allowing growth of chloramphenicol-resistant bacteria and, in many formulations, favors recovery of fungi. Columbia agar base is a complex peptone medium originally devised as a blood-agar base to cultivate both fastidious organisms (such as streptococci, staphylococci, Neisseria, and Haemophilus) and non-fastidious bacteria. The peptones and yeast extract provide amino acids, peptides, nitrogen, B-complex vitamins, and other growth factors that support rapid, luxuriant bacterial growth. Starch serves as a carbon and energy source and can bind or neutralize toxic metabolites, aiding the recovery of more delicate organisms, while sodium chloride maintains osmotic balance.
Chloramphenicol is a broad-spectrum antibiotic that binds to the 50S ribosomal subunit and inhibits peptidyl transferase activity, blocking peptide bond formation and thereby protein synthesis in susceptible bacteria. It inhibits many Gram-positive and Gram-negative bacteria but has much less effect on yeasts and molds. When chloramphenicol is added to Columbia Agar Base, the medium becomes selective: chloramphenicol-susceptible bacteria are suppressed or completely inhibited, while chloramphenicol-resistant bacteria and fungi can still grow.
* This product is manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision). All final packaging, quality assurance, and testing are done at the DiagnoCine R&D and Quality Testing Center. All specific customization requests and assembly were accomplished at DiagnoCine Precision in Totowa, New Jersey, USA
