TBS Blocking Buffer with Casein (1%)
TBS Blocking Buffer with Casein (1%)
TBS Blocking Buffer with Casein (1%) (DCP-CTBS1X) is a research-grade, single-protein blocking reagent formulated in Tris-Buffered Saline for Western blotting, ELISA, and immunohistochemistry. 0.1 µm filtration removes large particulates and aggregates, helping protect downstream applications. It provides a stable pH 7.4 environment with minimal non-specific background in immunoassays.
- 0.1 µm membrane filtration removes large particulates and aggregates
- 1% purified casein blocking agent minimizes non-specific binding and cross-reactivity
- Stable pH 7.4 Tris-Buffered Saline formulation (50 mM Tris-base, 150 mM NaCl)
- No DNase or RNase activity detected after 18 hr incubation at room temperature
- Formulated with Ultrapure Type 1 water (18.2 MΩ·cm)
- Manufactured under ISO 13485-certified facilities
- Compatible with Western blot, ELISA, and IHC workflows
- Custom concentrations, additives, and pH available on request
- pH7.4
- Formulation50 mM Tris-base, 150 mM NaCl
- Blocking Agent1% Casein
- AppearanceClear, colorless liquid
- Filtration0.1 µm membrane filtration (single step)
- SterilityNonsterile
- DNase ActivityNone detected
- RNase ActivityNone detected
- Storage-20°C
- Shelf Life1 year
Engineered where standard buffers fail
Particulates, inconsistent pH, and high background can compromise sensitive immunoassays. This buffer is designed to minimize these problems.
0.1 µm filtered
0.1 µm filtration removes large particulates and aggregates, helping protect downstream applications.
Precise, stable pH
Buffered at pH 7.4 in a defined Tris-Buffered Saline system (50 mM Tris-base, 150 mM NaCl) to protect protein and antibody integrity during assays.
Ultrapure-grade water
Formulated with Ultrapure Type 1 water (18.2 MΩ·cm) as the aqueous base for the buffer system.
Low background for imaging & assays
The 1% purified casein blocking layer minimizes non-specific interactions, lowering background in Western blot, ELISA, and IHC detection.
Defined, traceable composition
Every component is disclosed with concentration, and the buffer is confirmed free of detectable DNase and RNase activity.
Customization on demand
Alternate concentrations, additives, proteins, supplements, or pH values are available — contact support@diagnocine.com.
Single-stage 0.1 µm filtration
DCP-CTBS1X is processed through a 0.1 µm membrane filtration step, delivering a ready-to-use blocking buffer.
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0.1 µm Membrane Filtration
Removes large particulates and aggregates, helping protect downstream applications.
0.1 µm membrane filtration
0.1 µm filtration removes large particulates and aggregates, helping protect downstream applications.
© Diagnocine® — DCP-CTBS1X
Where DCP-CTBS1X performs
A single-protein casein blocking buffer designed to reduce non-specific binding across immunoassay, imaging, and immunostaining workflows.
Dot Blot & Protein Arrays
Blocks unoccupied binding sites on membranes before antibody probing in dot blots and protein arrays.
Blocking, Wash & Dilution
Applied to block membranes and microplates and to rinse between immunoassay steps.
iPSC-Derived Model Immunostaining
Blocks non-specific antibody binding during immunocytochemistry of iPSC-derived cell models.
Endothelial & Primary Cell Immunostaining
Reduces non-specific binding in immunofluorescence staining of endothelial and primary cells.
ELISA, Western Blot & IHC Blocking
Blocks non-specific binding sites on membranes and microplates, including protocols using biotin-avidin complexes and alkaline phosphatase detection.
Microscopy & Optical Sensing
Lowers assay background for microscopy and optical sensing workflows that follow blocking steps.
Full specification sheet
Every value below reflects DCP-CTBS1X as manufactured and released.
| Parameter | Specification |
|---|---|
| Formulation / Composition | 50 mM Tris-base, 150 mM NaCl, 1% Casein |
| Appearance | Clear, colorless liquid |
| pH | 7.4 |
| Buffering System | Tris-Buffered Saline (TBS) |
| Blocking Agent Concentration | 1% Casein |
| Parameter | Specification |
|---|---|
| Sterility | Nonsterile |
| Filtration | 0.1 µm membrane filtration (single step) |
| DNase Activity | None detected (18 hr incubation with plasmid DNA, room temperature) |
| RNase Activity | None detected (18 hr incubation with ribosomal RNA, room temperature) |
| Water Quality | Ultrapure Type 1 water (18.2 MΩ·cm) |
| Manufacturing Standard ISO 13485 | ISO 13485-certified facilities |
| Parameter | Specification |
|---|---|
| Storage Temperature | -20°C |
| Shelf Life | 1 year |
| Parameter | Specification |
|---|---|
| Raw Material Grade | Purified, optionally chemically modified casein (single-protein blocking agent) |
| Manufacturing QMS ISO 13485 | ISO 13485-certified facilities (DiagnoCine Precision suppliers) |
| Production & Customization Site | DiagnoCine R&D and Quality Testing Center; final assembly at DiagnoCine Precision, Totowa, New Jersey, USA |
| Intended Use | Research Use Only (RUO) |
Full composition
Component concentrations for DCP-CTBS1X as released, expressed in the units used at manufacture.
| Component | CAS Number | Concentration |
|---|---|---|
| Casein | 9000-71-9 | 1% |
| Tris-base | 77-86-1 | 50 mM |
| Sodium Chloride | 7647-14-5 | 150 mM |
Manufactured under controlled quality systems
DCP-CTBS1X is produced, packaged, and released under a documented quality management framework.
ISO 13485:2016 Quality Management
Manufactured under ISO 13485-certified facilities (Suppliers of DiagnoCine Precision).
Ultrapure Type 1 Water
Formulated using Ultrapure Type 1 water (18.2 MΩ·cm) as the aqueous base.
Controlled Fill & Finish
Final packaging, quality assurance, and testing performed at the DiagnoCine R&D and Quality Testing Center.
Micro-Batch Precision
Customization requests and assembly are completed at DiagnoCine Precision in Totowa, New Jersey, USA.
Endotoxin
See CoA.
Particulate (USP <788>)
Particulate control is supported by 0.1 µm membrane filtration within the release protocol.
Osmolality (USP <785>)
Osmolality is monitored as part of the buffer release framework aligned with USP <785>.
Documentation / CoA
Appearance, pH, and DNase/RNase test results are documented and available per lot.
How DCP-CTBS1X compares
A side-by-side look at DCP-CTBS1X against conventional casein and milk-based blocking buffers.
| Parameter | DCP-CTBS1X | Conventional blocking buffer | Standard alternative |
|---|---|---|---|
| Blocking Agent | 1% Purified Casein (single-protein) | Non-fat Milk/BSA Blend | Non-fat Milk |
| DNase / RNase Tested | check_circle | cancel | cancel |
| Water Quality | Ultrapure Type 1 (18.2 MΩ·cm) | Typical Type 2/3 water | Typical Type 2/3 water |
| ISO 13485-Certified Manufacturing | check_circle | cancel | cancel |
| Custom Formulation Available | check_circle | cancel | cancel |
Frequently asked questions
Answers to common technical questions about DCP-CTBS1X.
Supporting literature
Curated peer-reviewed literature relevant to blocking buffer chemistry and immunoassay background reduction.
- Mahmood, T., & Yang, P.C. Western blot: technique, theory, and trouble shooting. N Am J Med Sci. 2012. doi:10.4103/1947-2714.100998
- Kurien, B.T., & Scofield, R.H. Western blotting. Methods. 2006. doi:10.1016/j.ymeth.2005.07.018
- Vogt, R.F. Jr., et al. Quantitative differences among various proteins as blocking agents for ELISA microtiter plates. J Immunol Methods. 1987. doi:10.1016/0022-1759(87)90447-9
- Steinitz, M. Quantitation of the blocking effect of Tween 20 and bovine serum albumin (BSA) in ELISA microwells. Anal Biochem. 2000. doi:10.1006/abio.2000.4577
- Kenna, J.G., Major, G.N., & Williams, R.S. Methods for reducing non-specific antibody binding in enzyme-linked immunosorbent assays. J Immunol Methods. 1985. doi:10.1016/0022-1759(85)90042-0
- Good, N.E., et al. Hydrogen ion buffers for biological research. Biochemistry. 1966. doi:10.1021/bi00866a011
