CUBIC is a water-soluble tissue clearing reagent kit designed for PFA-fixed organs and tissues. It enables 3D imaging of entire organs at cellular resolution using Light-Sheet Fluorescence Microscopy (LSFM) or Confocal Laser Scanning Microscopy (CLSM). The protocol requires only standard laboratory equipment—no specialized equipments like electrophoresis systems are required.
CUBIC Technology was developed by RIKEN and the University of Tokyo over about 10 years to allow observation of all cells in organs and the body. Today, it is used by researchers around the world and gained high trust as a versatile tissue clearing and 3D tissue staining method.
Features
Productized based on CUBIC-HistoVIsion, the world’s highest-performance 3D tissue staining technology.
The kit includes reagents that allow equal staining of the inside and outside of the 3D samples.
To be used together with CUBIC clearing reagents (sold separately).
Available in a starter kit that includes a staining pot optimized for experiments and a general kit (the staining pot can be used repeatedly).
Kit Contents
Pre-wash buffer
Staining buffer
Additive A-F
Wrapping reagents
Supplement for staining buffer (supplement)
Application Example
3D Staining Mouse Organ Practical Eample
For other application examples, please refer to the technical guidebook under Manual.