FluxMPS™ Bafilomycin A (10µg/mL)
Microfluidics Suitable, dual-stage ultra-filtered (0.1 µm ×1 + 0.04 µm ×1) DMSO stock solution of Bafilomycin A1, a potent and selective vacuolar H+-ATPase (V-ATPase) inhibitor. Supplied ready-to-use at 10 µg/mL for direct dilution into autophagy, lysosome, endosome, and membrane-trafficking assays, including microfluidic and organ-on-a-chip (OoC) culture systems.
- Ready-to-use 10 µg/mL Bafilomycin A1 stock solution in DMSO for direct dilution into experimental systems
- Potent, selective vacuolar H+-ATPase (V-ATPase) inhibitor that blocks proton translocation into acidic organelles
- Widely used to distinguish increased autophagosome formation from impaired autophagic degradation in autophagy flux assays
- Processed through Diagnocine's dual-stage filtration architecture (0.1 µm → 0.04 µm) for microchannel-safe purity
- Confirmed free of DNase and RNase activity after 18-hour incubation testing with plasmid DNA and ribosomal RNA
- Manufactured under an ISO 13485:2016 quality management system; final packaging and QC performed at Diagnocine, Totowa, NJ
- Supplied as 10 mL (10 × 1 mL) single-use aliquots to minimize freeze-thaw cycles and preserve potency
- Custom concentrations, chemical/compound/protein/supplement additions, and pH modifications available on request
- Active CompoundBafilomycin A1
- Concentration10 µg/mL
- SolventDMSO (Dimethyl sulfoxide)
- AppearanceClear, colorless liquid
- DNase ActivityNone detected
- RNase ActivityNone detected
- Filtration0.1 µm ×1 + 0.04 µm ×1 (Dual-stage)
- Storage-80°C, in the dark
- Shelf Life12 months from date of manufacture, unopened
- Pack Size10 mL (10 × 1 mL)
Engineered where standard reagents fall short
Conventional 0.22 µm filtered research reagents can carry mycoplasma-scale particulates, subvisible aggregates, and inconsistent lot-to-lot purity — all of which can confound sensitive autophagy, lysosomal, and microfluidic assays. FluxMPS™ addresses these failure modes directly.
Microchannel-safe purity
Dual-stage 0.1 µm / 0.04 µm filtration removes particulates that can accumulate in narrow microfluidic channels and valves.
Precise mechanistic control
A single, well-characterized V-ATPase inhibitor delivered at a defined 10 µg/mL concentration for reproducible autophagy and lysosomal pH studies.
Controlled solvent system
Formulated in DMSO for consistent solubility and accurate low-volume dosing across experimental replicates.
Low background for imaging
Supplied as a clear, colorless liquid to minimize interference with confocal and other optical readouts of lysosomal and autophagosome markers.
Confirmed nuclease-free
Each lot is tested for DNase and RNase activity to protect nucleic-acid-based assays run alongside treatment.
Customization on demand
Alternate concentrations, additional chemicals, compounds, proteins, supplements, and pH adjustments available on request.
Dual-stage filtration system
Bafilomycin A (10µg/mL) is processed through Diagnocine's validated dual-stage filtration train, reaching a 0.04 µm final pore size. A dedicated 0.1 µm prefilter protects the 0.04 µm final filter, reducing particulate and mycoplasma-scale contamination risk ahead of final polish.
- 1
0.1 µm Prefiltration
Removes large particulates and aggregates from the DMSO solution, protecting the downstream 0.04 µm final filter.
- 2
0.04 µm Final filtration — Polish
Final polishing pass retaining sub-micron particulates and microaggregates that pass a conventional 0.22 µm filter.
Performance vs. conventional media
The dual-stage 0.1 µm / 0.04 µm train provides a finer, more consistent particulate cut-off than the single-stage 0.22 µm filtration typically used for standard research reagents.
Research applications
Bafilomycin A1 is a widely used pharmacological tool for probing autophagic flux, lysosomal acidification, and vesicular trafficking across a broad range of cell-based and microphysiological research systems.
Automated Bioreactors & Robotics
For automated dosing systems and long-duration perfusion bioreactors, Diagnocine also offers an optional 0.01 µm (10 nm) ultra nano-filtered MPS Grade variant of this reagent line, engineered to further reduce particulate load in automated fluid-handling systems.
- Total Particulate Exclusion for long-duration automated dosing lines
- Valve & Sensor Protection in closed-loop perfusion systems
- Extended Perfusion Stability for multi-week automated culture protocols
Inquiry Required: The 0.01 µm MPS Grade variant is available by request; contact support@diagnocine.com for specifications and lead time.
Micro Physiological System (MPS) & Chip
Direct dosing into closed microfluidic channels for autophagy and lysosomal pH studies.
Autophagy in Tumor Cell Models
Used to dissect autophagy-dependent survival and drug-resistance mechanisms in tumor cell lines.
Autophagy in iPSC-Derived Models
Applied to study autophagic flux during differentiation and maturation of iPSC-derived lineages.
Endothelial Autophagy & Trafficking
Used to probe lysosomal and endosomal trafficking in vascular and primary cell models.
Lysosomal Contribution to Metabolic Flux
Used alongside tracer-based methods to study how lysosomal inhibition alters cellular metabolism.
LC3 Flux & Lysosomal pH Imaging
Widely used in confocal and biosensor-based imaging assays of autophagosome-lysosome fusion.
Technical specifications
Physical, quality, storage, and regulatory parameters for Bafilomycin A (10µg/mL), DCP-BMA10UG.
| Parameter | Specification |
|---|---|
| Formulation | Bafilomycin A1 in DMSO |
| Concentration | 10 µg/mL |
| Appearance | Clear, colorless liquid |
| Solvent | Dimethyl sulfoxide (DMSO) |
| Parameter | Specification |
|---|---|
| Sterility | Filtered 0.1 µm membrane (single pass); designated Sterile per product documentation USP |
| Mycoplasma | 0.1 µm mycoplasma-retentive prefiltration (not tested per lot); smallest known mycoplasma species measure 0.2–0.3 µm |
| DNase Activity | None detected (18 hr incubation with plasmid DNA, room temperature) |
| RNase Activity | None detected (18 hr incubation with ribosomal RNA, room temperature) |
| Parameter | Specification |
|---|---|
| Storage Temperature | -80°C, in the dark |
| Shelf Life | 12 months from date of manufacture, unopened |
| Pack Size | 10 mL (10 × 1 mL) |
| Shipping Condition | Contact for specification |
| Parameter | Specification |
|---|---|
| Manufacturing QMS | ISO 13485-certified suppliers ISO; final packaging, QA, and testing at Diagnocine |
| UNSPSC | 41116155 · Molecular biology and cell culture growth media (UNv260801) |
| Regulatory Alignment | ISO 13485:2016 |
| Production Method | Custom assembly and QC at Diagnocine Precision, Totowa, New Jersey, USA |
| Intended Use | For Research Use Only (RUO) |
Full composition
Bafilomycin A (10µg/mL) is a two-component formulation: the active pharmacological compound Bafilomycin A1 dissolved in a DMSO vehicle. Lot-release quality control attributes are listed in the third tab.
| Component | CAS Number | Concentration |
|---|---|---|
| Bafilomycin A1 | 88899-55-2 | 10 µg/mL |
| Component | CAS Number | Concentration |
|---|---|---|
| Dimethyl sulfoxide (DMSO) | 67-68-5 | Vehicle, q.s. to volume |
| Attribute | Method | Result |
|---|---|---|
| Appearance | Visual inspection | Clear, colorless liquid |
| DNase activity | Plasmid DNA incubation, 18 hr, room temperature | None detected |
| RNase activity | Ribosomal RNA incubation, 18 hr, room temperature | None detected |
Manufacturing & compliance
Bafilomycin A (10µg/mL) is produced under a controlled quality system with lot-level functional testing and final release performed at Diagnocine.
ISO 13485 QMS
Manufactured under ISO 13485-certified suppliers of Diagnocine Precision.
Dual-stage filtration
Processed through a 0.1 µm prefilter and 0.04 µm final filter for microchannel-safe purity.
DNase / RNase Tested
Each lot is confirmed free of detectable DNase and RNase activity after 18-hour incubation testing.
Finished in Totowa, NJ
Final packaging, quality assurance, testing, and customization are completed at the Diagnocine R&D and Quality Testing Center, Totowa, New Jersey, USA.
Sterility
Filtered through a 0.1 µm membrane (single pass); designated Sterile per product documentation.
Functional Purity Testing
DNase and RNase activity testing performed on each lot using plasmid DNA and ribosomal RNA substrates.
Custom Formulation
Alternate concentrations, additional chemicals, compounds, proteins, supplements, and pH adjustments available on request.
Documentation
Certificate of Analysis available on request, including lot number and expiry.
How DCP-BMA10UG compares
A comparison of FluxMPS™ Bafilomycin A (10µg/mL) against representative classical cell-culture-grade reagent suppliers.
| Parameter | DCP-BMA10UG (FluxMPS™) | Other Suppliers |
|---|---|---|
| Grade | Microfluidics Suitable | Varies by supplier; not typically specified |
| Filtration architecture | Dual-stage: 0.1 µm ×1 + 0.04 µm ×1 | Typically single-stage 0.22 µm filtration |
| Mycoplasma risk mitigation | check_circle 0.1 µm mycoplasma-retentive prefiltration | cancel Not typically stated at 0.22 µm |
| Endotoxin (release specification) | FluxMPS™ — Not specified | Corning classical liquid media — < 0.25 EU/mL Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL Gibco classical DMEM — Not specified (recorded per lot) |
| DNase / RNase testing | check_circle Tested, none detected | Varies by supplier |
| Manufacturing QMS | ISO 13485:2016 | Varies by supplier |
| Custom formulation | check_circle Available on request | Limited or not offered |
Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".
Frequently asked questions
Common questions about Bafilomycin A (10µg/mL), DCP-BMA10UG.
Supporting literature
Key references on Bafilomycin A1 pharmacology, autophagy assays, and microfluidic/organ-on-a-chip research applications.
- Bowman EJ, Siebers A, Altendorf K. Bafilomycins: a class of inhibitors of membrane ATPases from microorganisms, animal cells, and plant cells. Proc Natl Acad Sci USA. 1988;85(21):7972-7976. doi:10.1073/pnas.85.21.7972
- Yoshimori T, Yamamoto A, Moriyama Y, Futai M, Tashiro Y. Bafilomycin A1, a specific inhibitor of vacuolar-type H(+)-ATPase, inhibits acidification and protein degradation in lysosomes of cultured cells. J Biol Chem. 1991;266(26):17707-17712. doi:10.1016/S0021-9258(19)47429-2
- Yamamoto A, Tagawa Y, Yoshimori T, Moriyama Y, Masaki R, Tashiro Y. Bafilomycin A1 prevents maturation of autophagic vacuoles by inhibiting fusion between autophagosomes and lysosomes in rat hepatoma cell line, H-4-II-E cells. Cell Struct Funct. 1998;23(1):33-42. doi:10.1247/csf.23.33
- Mauvezin C, Neufeld TP. Bafilomycin A1 disrupts autophagic flux by inhibiting both V-ATPase-dependent acidification and Ca-P60A/SERCA-dependent autophagosome-lysosome fusion. Autophagy. 2015;11(8):1437-1438. doi:10.1080/15548627.2015.1066957
- Klionsky DJ, et al. Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition). Autophagy. 2021;17(1):1-382. doi:10.1080/15548627.2020.1797280
- Mizushima N, Yoshimori T, Levine B. Methods in mammalian autophagy research. Cell. 2010;140(3):313-326. doi:10.1016/j.cell.2010.01.028
- Huh D, Matthews BD, Mammoto A, Montoya-Zavala M, Hsin HY, Ingber DE. Reconstituting organ-level lung functions on a chip. Science. 2010;328(5986):1662-1668. doi:10.1126/science.1188302
- Huss M, Ingenhorst G, Konig S, et al. Concanamycin A, the specific inhibitor of V-ATPases, binds to the V(o) subunit c. J Biol Chem. 2002;277(43):40544-40548. doi:10.1074/jbc.M207345200
- Klionsky DJ, Elazar Z, Seglen PO, Rubinsztein DC. Does bafilomycin A1 block the fusion of autophagosomes with lysosomes? Autophagy. 2008;4(7):849-950. doi:10.4161/auto.6845
